@article{RudolphMohrBereswillToetzkeetal.2021,
  author    = {Rudolph-Mohr, Nicole and Bereswill, Sarah and T{\"o}tzke, Christian and Kardjilov, Nikolay and Oswald, Sascha},
  title     = {Neutron computed laminography yields 3D root system architecture and complements investigations of spatiotemporal rhizosphere patterns},
  series = {Plant and soil},
  volume    = {469},
  journal   = {Plant and soil},
  number    = {1-2},
  publisher = {Springer},
  address   = {Dordrecht},
  issn      = {0032-079X},
  doi       = {10.1007/s11104-021-05120-7},
  pages     = {489 -- 501},
  year      = {2021},
  abstract  = {Purpose Root growth, respiration, water uptake as well as root exudation induce biogeochemical patterns in the rhizosphere that can change dynamically over time. Our aim is to develop a method that provides complementary information on 3D root system architecture and biogeochemical gradients around the roots needed for the quantitative description of rhizosphere processes. Methods We captured for the first time the root system architecture of maize plants grown in rectangular rhizotrons in 3D using neutron computed laminography (NCL). Simultaneously, we measured pH and oxygen concentration using fluorescent optodes and the 2D soil water distribution by means of neutron radiography. We co-registered the 3D laminography data with the 2D oxygen and pH maps to analyze the sensor signal as a function of the distance between the roots and the optode. Results The 3D root system architecture was successfully segmented from the laminographic data. We found that exudation of roots in up to 2 mm distance to the pH optode induced patterns of local acidification or alkalization. Over time, oxygen gradients in the rhizosphere emerged for roots up to a distance of 7.5 mm. Conclusion Neutron computed laminography allows for a three-dimensional investigation of root systems grown in laterally extended rhizotrons as the ones designed for 2D optode imaging studies. The 3D information on root position within the rhizotrons derived by NCL explained measured 2D oxygen and pH distribution. The presented new combination of 3D and 2D imaging methods facilitates systematical investigations of a wide range of dynamic processes in the rhizosphere.},
  language  = {en}
}
@article{ToetzkeOswaldHilgeretal.2021,
  author    = {T{\"o}tzke, Christian and Oswald, Sascha and Hilger, Andr{\´e} and Kardjilov, Nikolay},
  title     = {Non-invasive detection and localization of microplastic particles in a sandy sediment by complementary neutron and X-ray tomography},
  series = {Journal of soils and sediments : protection, risk assessment and remediation},
  volume    = {21},
  journal   = {Journal of soils and sediments : protection, risk assessment and remediation},
  number    = {3},
  publisher = {Springer},
  address   = {Berlin ; Heidelberg},
  issn      = {1439-0108},
  doi       = {10.1007/s11368-021-02882-6},
  pages     = {1476 -- 1487},
  year      = {2021},
  abstract  = {Purpose Microplastics have become a ubiquitous pollutant in marine, terrestrial and freshwater systems that seriously affects aquatic and terrestrial ecosystems. Common methods for analysing microplastic abundance in soil or sediments are based on destructive sampling or involve destructive sample processing. Thus, substantial information about local distribution of microplastics is inevitably lost. Methods Tomographic methods have been explored in our study as they can help to overcome this limitation because they allow the analysis of the sample structure while maintaining its integrity. However, this capability has not yet been exploited for detection of environmental microplastics. We present a bimodal 3D imaging approach capable to detect microplastics in soil or sediment cores non-destructively. Results In a first pilot study, we demonstrate the unique potential of neutrons to sense and localize microplastic particles in sandy sediment. The complementary application of X-rays allows mineral grains to be discriminated from microplastic particles. Additionally, it yields detailed information on the 3D surroundings of each microplastic particle, which supports its size and shape determination. Conclusion The procedure we developed is able to identify microplastic particles with diameters of approximately 1 mm in a sandy soil. It also allows characterisation of the shape of the microplastic particles as well as the microstructure of the soil and sediment sample as depositional background information. Transferring this approach to environmental samples presents the opportunity to gain insights of the exact distribution of microplastics as well as their past deposition, deterioration and translocation processes.},
  language  = {en}
}
@article{ToetzkeKardjilovHilgeretal.2021,
  author    = {T{\"o}tzke, Christian and Kardjilov, Nikolay and Hilger, Andr{\´e} and Rudolph-Mohr, Nicole and Manke, Ingo and Oswald, Sascha},
  title     = {Three-dimensional in vivo analysis of water uptake and translocation in maize roots by fast neutron tomography},
  series = {Scientific Reports},
  volume    = {11},
  journal   = {Scientific Reports},
  publisher = {Macmillan Publishers Limited},
  address   = {London},
  issn      = {2045-2322},
  doi       = {10.1038/s41598-021-90062-4},
  pages     = {10},
  year      = {2021},
  abstract  = {Root water uptake is an essential process for terrestrial plants that strongly affects the spatiotemporal distribution of water in vegetated soil. Fast neutron tomography is a recently established non-invasive imaging technique capable to capture the 3D architecture of root systems in situ and even allows for tracking of three-dimensional water flow in soil and roots. We present an in vivo analysis of local water uptake and transport by roots of soil-grown maize plants—for the first time measured in a three-dimensional time-resolved manner. Using deuterated water as tracer in infiltration experiments, we visualized soil imbibition, local root uptake, and tracked the transport of deuterated water throughout the fibrous root system for a day and night situation. This revealed significant differences in water transport between different root types. The primary root was the preferred water transport path in the 13-days-old plants while seminal roots of comparable size and length contributed little to plant water supply. The results underline the unique potential of fast neutron tomography to provide time-resolved 3D in vivo information on the water uptake and transport dynamics of plant root systems, thus contributing to a better understanding of the complex interactions of plant, soil and water.},
  language  = {en}
}
@misc{ToetzkeKardjilovHilgeretal.2021,
  author    = {T{\"o}tzke, Christian and Kardjilov, Nikolay and Hilger, Andr{\´e} and Rudolph-Mohr, Nicole and Manke, Ingo and Oswald, Sascha},
  title     = {Three-dimensional in vivo analysis of water uptake and translocation in maize roots by fast neutron tomography},
  series = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe},
  journal   = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe},
  issn      = {1866-8372},
  doi       = {10.25932/publishup-52991},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-529915},
  pages     = {12},
  year      = {2021},
  abstract  = {Root water uptake is an essential process for terrestrial plants that strongly affects the spatiotemporal distribution of water in vegetated soil. Fast neutron tomography is a recently established non-invasive imaging technique capable to capture the 3D architecture of root systems in situ and even allows for tracking of three-dimensional water flow in soil and roots. We present an in vivo analysis of local water uptake and transport by roots of soil-grown maize plants—for the first time measured in a three-dimensional time-resolved manner. Using deuterated water as tracer in infiltration experiments, we visualized soil imbibition, local root uptake, and tracked the transport of deuterated water throughout the fibrous root system for a day and night situation. This revealed significant differences in water transport between different root types. The primary root was the preferred water transport path in the 13-days-old plants while seminal roots of comparable size and length contributed little to plant water supply. The results underline the unique potential of fast neutron tomography to provide time-resolved 3D in vivo information on the water uptake and transport dynamics of plant root systems, thus contributing to a better understanding of the complex interactions of plant, soil and water.},
  language  = {en}
}