@article{SchellenbergReichertHardtetal.2020,
  author    = {Schellenberg, Johannes and Reichert, Jessica and Hardt, Martin and Klingelh{\"o}fer, Ines and Morlock, Gertrud and Schubert, Patrick and Bižić, Mina and Grossart, Hans-Peter and K{\"a}mpfer, Peter and Wilke, Thomas and Glaeser, Stefanie P.},
  title     = {The bacterial microbiome of the long-term aquarium cultured high-microbial abundance sponge Haliclona cnidata},
  series = {Frontiers in Marine Science},
  volume    = {7},
  journal   = {Frontiers in Marine Science},
  publisher = {Frontiers Media},
  address   = {Lausanne},
  issn      = {2296-7745},
  doi       = {10.3389/fmars.2020.00266},
  pages     = {20},
  year      = {2020},
  abstract  = {Marine sponges host highly diverse but specific bacterial communities that provide essential functions for the sponge holobiont, including antimicrobial defense. Here, we characterized the bacterial microbiome of the marine sponge Haliclona cnidata that has been in culture in an artificial marine aquarium system. We tested the hypotheses (1) that the long-term aquarium cultured sponge H. cnidata is tightly associated with a typical sponge bacterial microbiota and (2) that the symbiotic Bacteria sustain bioactivity under harmful environmental conditions to facilitate holobiont survival by preventing pathogen invasion. Microscopic and phylogenetic analyses of the bacterial microbiota revealed that H. cnidata represents a high microbial abundance (HMA) sponge with a temporally stable bacterial community that significantly shifts with changing aquarium conditions. A 4-week incubation experiment was performed in small closed aquarium systems with antibiotic and/or light exclusion treatments to reduce the total bacterial and photosynthetically active sponge-associated microbiota to a treatment-specific resilient community. While the holobiont was severely affected by the experimental treatment (i.e., bleaching of the sponge, reduced bacterial abundance, shifted bacterial community composition), the biological defense and bacterial community interactions (i.e., quorum sensing activity) remained intact. 16S rRNA gene amplicon sequencing revealed a resilient community of 105 bacterial taxa, which remained in the treated sponges. These 105 taxa accounted for a relative abundance of 72-83\% of the bacterial sponge microbiota of non-treated sponge fragments that have been cultured under the same conditions. We conclude that a sponge-specific resilient community stays biologically active under harmful environmental conditions, facilitating the resilience of the holobiont. In H. cnidata, bacteria are located in bacteriocytes, which may have contributed to the observed phenomenon.},
  language  = {en}
}
@article{GlaeserBolteMartinetal.2013,
  author    = {Gl{\"a}ser, Stefanie P. and Bolte, Kathrin and Martin, Karin and Busse, Hans-J{\"u}rgen and Grossart, Hans-Peter and K{\"a}mpfer, Peter and Gl{\"a}ser, Jens},
  title     = {Novosphingobium fuchskuhlense sp nov., isolated from the north-east basin of Lake Grosse Fuchskuhle},
  series = {International journal of systematic and evolutionary microbiology},
  volume    = {63},
  journal   = {International journal of systematic and evolutionary microbiology},
  publisher = {Society for General Microbiology},
  address   = {Reading},
  issn      = {1466-5026},
  doi       = {10.1099/ijs.0.043083-0},
  pages     = {586 -- 592},
  year      = {2013},
  abstract  = {A yellow pigmented, Gram-negative, rod-shaped bacterium designated FNE08-7(T) was isolated from subsurface water of the north-east basin of the bog lake Grosse Fuchskuhle (Brandenburg, Germany). A first analysis of the nearly full-length 16S rRNA gene sequence analysis including environmental 16S rRNA gene sequences derived from freshwater ecosystems showed that strain FNE08-7(T) is the first cultured representative, to our knowledge, of the freshwater tribe Novo-A2. Further analysis indicates highest 16S rRNA gene sequence similarities to the type strains of Novosphingobium stygium (98.0\%) and Novosphingobium taihuense (97.4\%) and between 94.0\% and 96.9\% sequence similarity to other members of the genus Novosphingobium. Reconstruction of phylogenetic trees showed that strain FNE08-7(T) formed a distinct cluster with the type strains of N. stygium and N. taihuense supported by high bootstrap values. DNA DNA hybridization of strain FNE08-7(T) with N. stygium SMCC B0712(T) and N. taihuense DSM 17507(T) revealed low similarity values of 18.4\% (reciprocal: 11.4\%) and 23.1\% (reciprocal: 54.2\%), respectively. The predominant fatty acid of the isolate is C-18:1 omega 7c (56.4\%) and two characteristic 2-hydroxy fatty acids, C-14:0 2-OH (16.5\%) and C-15:0 2-OH (3.3\%) occur. Ubiquinone Q-10 is the major respiratory quinone. The predominant polar lipids are phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylglycerol, sphingoglycolipid, phosphatidylcholine and minor amounts of diphosphatidylglycerol. Spermidine is the predominant polyamine. Characterization by genotypic, chemotaxonomic and phenotypic analysis indicate that strain FNE08-7(T) represents a novel species of the genus Novosphingobium within the Alphaproteobacteria. Therefore, we propose the species Novosphingobium fuchskuhlense sp. nov., with FNE08-7(T) (=DSM 25065(T)=CCM 7978(T)=CCUG 61508(T)) as the type strain.},
  language  = {en}
}