@article{BalazadehSiddiquiAlluetal.2010,
  author    = {Balazadeh, Salma and Siddiqui, Hamad and Allu, Annapurna Devi and Matallana-Ramirez, Lilian Paola and Caldana, Camila and Mehrnia, Mohammad and Zanor, Maria-In{\´e}s and Koehler, Barbara and M{\"u}ller-R{\"o}ber, Bernd},
  title     = {A gene regulatory network controlled by the NAC transcription factor ANAC092/AtNAC2/ORE1 during salt-promoted senescence},
  issn      = {0960-7412},
  doi       = {10.1111/j.1365-313X.2010.04151.x},
  year      = {2010},
  abstract  = {P>The onset and progression of senescence are under genetic and environmental control. The Arabidopsis thaliana NAC transcription factor ANAC092 (also called AtNAC2 and ORE1) has recently been shown to control age-dependent senescence, but its mode of action has not been analysed yet. To explore the regulatory network administered by ANAC092 we performed microarray-based expression profiling using estradiol-inducible ANAC092 overexpression lines. Approximately 46\% of the 170 genes up-regulated upon ANAC092 induction are known senescence-associated genes, suggesting that the NAC factor exerts its role in senescence through a regulatory network that includes many of the genes previously reported to be senescence regulated. We selected 39 candidate genes and confirmed their time-dependent response to enhanced ANAC092 expression by quantitative RT-PCR. We also found that the majority of them (24 genes) are up-regulated by salt stress, a major promoter of plant senescence, in a manner similar to that of ANAC092, which itself is salt responsive. Furthermore, 24 genes like ANAC092 turned out to be stage-dependently expressed during seed growth with low expression at early and elevated expression at late stages of seed development. Disruption of ANAC092 increased the rate of seed germination under saline conditions, whereas the opposite occurred in respective overexpression plants. We also detected a delay of salinity-induced chlorophyll loss in detached anac092-1 mutant leaves. Promoter-reporter (GUS) studies revealed transcriptional control of ANAC092 expression during leaf and flower ageing and in response to salt stress. We conclude that ANAC092 exerts its functions during senescence and seed germination through partly overlapping target gene sets.},
  language  = {en}
}
@misc{DortayMuellerRoeber2010,
  author    = {Dortay, Hakan and M{\"u}ller-R{\"o}ber, Bernd},
  title     = {A highly efficient pipeline for protein expression in Leishmania tarentolae sing infrared fluorescence protein as marker},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-44773},
  year      = {2010},
  abstract  = {Background: Leishmania tarentolae, a unicellular eukaryotic protozoan, has been established as a novel host for recombinant protein production in recent years. Current protocols for protein expression in Leishmania are, however, time consuming and require extensive lab work in order to identify well-expressing cell lines. Here we established an alternative protein expression work-flow that employs recently engineered infrared fluorescence protein (IFP) as a suitable and easy-to-handle reporter protein for recombinant protein expression in Leishmania. As model proteins we tested three proteins from the plant Arabidopsis thaliana, including a NAC and a type-B ARR transcription factor. Results: IFP and IFP fusion proteins were expressed in Leishmania and rapidly detected in cells by deconvolution microscopy and in culture by infrared imaging of 96-well microtiter plates using small cell culture volumes (2 μL - 100 μL). Motility, shape and growth of Leishmania cells were not impaired by intracellular accumulation of IFP. In-cell detection of IFP and IFP fusion proteins was straightforward already at the beginning of the expression pipeline and thus allowed early pre-selection of well-expressing Leishmania clones. Furthermore, IFP fusion proteins retained infrared fluorescence after electrophoresis in denaturing SDS-polyacrylamide gels, allowing direct in-gel detection without the need to disassemble cast protein gels. Thus, parameters for scaling up protein production and streamlining purification routes can be easily optimized when employing IFP as reporter. Conclusions: Using IFP as biosensor we devised a protocol for rapid and convenient protein expression in Leishmania tarentolae. Our expression pipeline is superior to previously established methods in that it significantly reduces the hands-on-time and work load required for identifying well-expressing clones, refining protein production parameters and establishing purification protocols. The facile in-cell and in-gel detection tools built on IFP make Leishmania amenable for high-throughput expression of proteins from plant and animal sources.},
  language  = {en}
}
@article{DortayMuellerRoeber2010,
  author    = {Dortay, Hakan and M{\"u}ller-R{\"o}ber, Bernd},
  title     = {A highly efficient pipeline for protein expression in Leishmania tarentolae using infrared fluorescence protein as marker},
  issn      = {1475-2859},
  doi       = {10.1186/1475-2859-9-29},
  year      = {2010},
  abstract  = {Background: Leishmania tarentolae, a unicellular eukaryotic protozoan, has been established as a novel host for recombinant protein production in recent years. Current protocols for protein expression in Leishmania are, however, time consuming and require extensive lab work in order to identify well-expressing cell lines. Here we established an alternative protein expression work-flow that employs recently engineered infrared fluorescence protein (IFP) as a suitable and easy-to-handle reporter protein for recombinant protein expression in Leishmania. As model proteins we tested three proteins from the plant Arabidopsis thaliana, including a NAC and a type-B ARR transcription factor. Results: IFP and IFP fusion proteins were expressed in Leishmania and rapidly detected in cells by deconvolution microscopy and in culture by infrared imaging of 96-well microtiter plates using small cell culture volumes (2 mu L},
  language  = {en}
}
@article{VoelkerGomezPorrasBeckeretal.2010,
  author    = {Voelker, Camilla and Gomez-Porras, Judith Lucia and Becker, Dirk and Hamamoto, Shin and Uozumi, Nobuyuki and Gambale, Franco and M{\"u}ller-R{\"o}ber, Bernd and Czempinski, Katrin and Dreyer, Ingo},
  title     = {Roles of tandem-pore K plus channels in plants : a puzzle still to be solved},
  issn      = {1435-8603},
  doi       = {10.1111/j.1438-8677.2010.00353.x},
  year      = {2010},
  abstract  = {The group of voltage-independent K+ channels in Arabidopsis thaliana consists of six members, five tandem-pore channels (TPK1-TPK5) and a single K-ir-like channel (KCO3). All TPK/KCO channels are located at the vacuolar membrane except for TPK4, which was shown to be a plasma membrane channel in pollen. The vacuolar channels interact with 14-3-3 proteins (also called General Regulating Factors, GRFs), indicating regulation at the level of protein-protein interactions. Here we review current knowledge about these ion channels and their genes, and highlight open questions that need to be urgently addressed in future studies to fully appreciate the physiological functions of these ion channels.},
  language  = {en}
}