@misc{MorrisSaltRailaetal.2012, author = {Morris, Penelope J. and Salt, Carina and Raila, Jens and Brenten, Thomas and Kohn, Barbara and Schweigert, Florian J. and Zentek, J{\"u}rgen}, title = {Safety evaluation of vitamin A in growing dogs}, series = {Potsprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe}, journal = {Potsprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe}, number = {686}, doi = {10.25932/publishup-41492}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-414929}, pages = {10}, year = {2012}, abstract = {The safe upper limit for inclusion of vitamin A in complete diets for growing dogs is uncertain, with the result that current recommendations range from 5.24 to 104.80 mu mol retinol (5000 to 100 000 IU vitamin A)/4184 kJ (1000 kcal) metabolisable energy (ME). The aim of the present study was to determine the effect of feeding four concentrations of vitamin A to puppies from weaning until 1 year of age. A total of forty-nine puppies, of two breeds, Labrador Retriever and Miniature Schnauzer, were randomly assigned to one of four treatment groups. Following weaning at 8 weeks of age, puppies were fed a complete food supplemented with retinyl acetate diluted in vegetable oil and fed at 1ml oil/100 g diet to achieve an intake of 5.24, 13.10, 78.60 and 104.80 mu mol retinol (5000, 12 500, 75 000 and 100 000 IU vitamin A)/4184 kJ (1000 kcal) ME. Fasted blood and urine samples were collected at 8, 10, 12, 14, 16, 20, 26, 36 and 52 weeks of age and analysed for markers of vitamin A metabolism and markers of safety including haematological and biochemical variables, bone-specific alkaline phosphatase, cross-linked carboxyterminal telopeptides of type I collagen and dual-energy X-ray absorptiometry. Clinical examinations were conducted every 4 weeks. Data were analysed by means of a mixed model analysis with Bonferroni corrections for multiple endpoints. There was no effect of vitamin A concentration on any of the parameters, with the exception of total serum retinyl esters, and no effect of dose on the number, type and duration of adverse events. We therefore propose that 104.80 mu mol retinol (100 000 IU vitamin A)/4184 kJ (1000 kcal) is a suitable safe upper limit for use in the formulation of diets designed for puppy growth.}, language = {en} } @misc{CaliendoHogenacker2012, author = {Caliendo, Marco and Hogenacker, Jens}, title = {The German labor market after the Great Recession}, series = {Postprints der Universit{\"a}t Potsdam : Wirtschafts- und Sozialwissenschaftliche Reihe}, journal = {Postprints der Universit{\"a}t Potsdam : Wirtschafts- und Sozialwissenschaftliche Reihe}, number = {129}, issn = {1867-5808}, doi = {10.25932/publishup-43519}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-435195}, pages = {26}, year = {2012}, abstract = {The reaction of the German labor market to the Great Recession 2008/09 was relatively mild - especially compared to other countries. The reason lies not only in the specific type of the recession - which was favorable for the German economy structure - but also in a series of labor market reforms initiated between 2002 and 2005 altering, inter alia, labor supply incentives. However, irrespective of the mild response to the Great Recession, there are a number of substantial future challenges the German labor market will soon have to face. Female labor supply still lies well below that of other countries and a massive demographic change over the next 50 years will have substantial effects on labor supply as well as the pension system. In addition, due to a skill-biased technological change over the next decades, firms will face problems of finding employees with adequate skills. The aim of this paper is threefold. First, we outline why the German labor market reacted in such a mild fashion, describe current economic trends of the labor market in light of general trends in the European Union, and reveal some of the main associated challenges. Thereafter, the paper analyzes recent reforms of the main institutional settings of the labor market which influence labor supply. Finally, based on the status quo of these institutional settings, the paper gives a brief overview of strategies to combat adequately the challenges in terms of labor supply and to ensure economic growth in the future.}, language = {en} } @misc{AdamBovend'EerdtDoorenetal.2012, author = {Adam, Jos J. and Bovend'Eerdt, Thamar J. H. and Dooren, Fleur E. P. van and Fischer, Martin H. and Pratt, Jay}, title = {The closer the better}, series = {Postprints der Universit{\"a}t Potsdam : Humanwissenschaftliche Reihe}, journal = {Postprints der Universit{\"a}t Potsdam : Humanwissenschaftliche Reihe}, number = {607}, issn = {1866-8364}, doi = {10.25932/publishup-43296}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-432963}, pages = {1533 -- 1538}, year = {2012}, abstract = {A growing literature has suggested that processing of visual information presented near the hands is facilitated. In this study, we investigated whether the near-hands superiority effect also occurs with the hands moving. In two experiments, participants performed a cyclical bimanual movement task requiring concurrent visual identification of briefly presented letters. For both the static and dynamic hand conditions, the results showed improved letter recognition performance with the hands closer to the stimuli. The finding that the encoding advantage for near-hand stimuli also occurred with the hands moving suggests that the effect is regulated in real time, in accordance with the concept of a bimodal neural system that dynamically updates hand position in external space.}, language = {en} } @misc{EbertLamprechtSteffenetal.2012, author = {Ebert, Birgitta E. and Lamprecht, Anna-Lena and Steffen, Bernhard and Blank, Lars M.}, title = {Flux-P}, series = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe}, journal = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe}, number = {1054}, issn = {1866-8372}, doi = {10.25932/publishup-47669}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-476696}, pages = {872 -- 890}, year = {2012}, abstract = {Quantitative knowledge of intracellular fluxes in metabolic networks is invaluable for inferring metabolic system behavior and the design principles of biological systems. However, intracellular reaction rates can not often be calculated directly but have to be estimated; for instance, via 13C-based metabolic flux analysis, a model-based interpretation of stable carbon isotope patterns in intermediates of metabolism. Existing software such as FiatFlux, OpenFLUX or 13CFLUX supports experts in this complex analysis, but requires several steps that have to be carried out manually, hence restricting the use of this software for data interpretation to a rather small number of experiments. In this paper, we present Flux-P as an approach to automate and standardize 13C-based metabolic flux analysis, using the Bio-jETI workflow framework. Exemplarily based on the FiatFlux software, it demonstrates how services can be created that carry out the different analysis steps autonomously and how these can subsequently be assembled into software workflows that perform automated, high-throughput intracellular flux analysis of high quality and reproducibility. Besides significant acceleration and standardization of the data analysis, the agile workflow-based realization supports flexible changes of the analysis workflows on the user level, making it easy to perform custom analyses.}, language = {en} } @misc{JingAmbroseKnoxetal.2012, author = {Jing, Runchun and Ambrose, Michael A. and Knox, Maggie R. and Smykal, Petr and Hybl, Miroslav and Ramos, {\´A}. and Caminero, Constantino and Burstin, Judith and Duc, Gerard and van Soest, L. J. M. and Święcicki, W. K. and Pereira, M. Graca and Vishnyakova, Margarita and Davenport, Guy F. and Flavell, Andrew J. and Ellis, T. H. Noel}, title = {Genetic diversity in European Pisum germplasm collections}, series = {Postprints der Universit{\"a}t Potsdam : Mathematisch Naturwissenschaftliche Reihe}, journal = {Postprints der Universit{\"a}t Potsdam : Mathematisch Naturwissenschaftliche Reihe}, number = {871}, issn = {1866-8372}, doi = {10.25932/publishup-43474}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-434743}, pages = {367 -- 380}, year = {2012}, abstract = {The distinctness of, and overlap between, pea genotypes held in several Pisum germplasm collections has been used to determine their relatedness and to test previous ideas about the genetic diversity of Pisum. Our characterisation of genetic diversity among 4,538 Pisum accessions held in 7 European Genebanks has identified sources of novel genetic variation, and both reinforces and refines previous interpretations of the overall structure of genetic diversity in Pisum. Molecular marker analysis was based upon the presence/absence of polymorphism of retrotransposon insertions scored by a high-throughput microarray and SSAP approaches. We conclude that the diversity of Pisum constitutes a broad continuum, with graded differentiation into sub-populations which display various degrees of distinctness. The most distinct genetic groups correspond to the named taxa while the cultivars and landraces of Pisum sativum can be divided into two broad types, one of which is strongly enriched for modern cultivars. The addition of germplasm sets from six European Genebanks, chosen to represent high diversity, to a single collection previously studied with these markers resulted in modest additions to the overall diversity observed, suggesting that the great majority of the total genetic diversity collected for the Pisum genus has now been described. Two interesting sources of novel genetic variation have been identified. Finally, we have proposed reference sets of core accessions with a range of sample sizes to represent Pisum diversity for the future study and exploitation by researchers and breeders.}, language = {en} } @misc{ChengVicente2012, author = {Cheng, Lisa Lai-Shen and Vicente, Luis}, title = {Verb doubling in Mandarin Chinese}, series = {Postprints der Universit{\"a}t Potsdam : Humanwissenschaftliche Reihe}, journal = {Postprints der Universit{\"a}t Potsdam : Humanwissenschaftliche Reihe}, number = {629}, issn = {1866-8364}, doi = {10.25932/publishup-43688}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-436880}, pages = {1 -- 37}, year = {2012}, abstract = {This article examines two so-far-understudied verb doubling constructions in Mandarin Chinese, viz., verb doubling clefts and verb doubling lian…dou. We show that these constructions have the same internal syntax as regular clefts and lian…dou sentences, the doubling effect being epiphenomenal; therefore, we classify them as subtypes of the general cleft and lian…dou constructions, respectively, rather than as independent constructions. Additionally, we also show that, as in many other languages with comparable constructions, the two instances of the verb are part of a single movement chain, which has the peculiarity of allowing Spell-Out of more than one link.}, language = {en} } @misc{HischeLarhlimiSchwarzetal.2012, author = {Hische, Manuela and Larhlimi, Abdelhalim and Schwarz, Franziska and Fischer-Rosinsk{\´y}, Antje and Bobbert, Thomas and Assmann, Anke and Catchpole, Gareth S. and Pfeiffer, Andreas F. H. and Willmitzer, Lothar and Selbig, Joachim and Spranger, Joachim}, title = {A distinct metabolic signature predictsdevelopment of fasting plasma glucose}, series = {Postprints der Universit{\"a}t Potsdam : Mathematisch Naturwissenschaftliche Reihe}, journal = {Postprints der Universit{\"a}t Potsdam : Mathematisch Naturwissenschaftliche Reihe}, number = {850}, issn = {1866-8372}, doi = {10.25932/publishup-42740}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-427400}, pages = {12}, year = {2012}, abstract = {Background High blood glucose and diabetes are amongst the conditions causing the greatest losses in years of healthy life worldwide. Therefore, numerous studies aim to identify reliable risk markers for development of impaired glucose metabolism and type 2 diabetes. However, the molecular basis of impaired glucose metabolism is so far insufficiently understood. The development of so called 'omics' approaches in the recent years promises to identify molecular markers and to further understand the molecular basis of impaired glucose metabolism and type 2 diabetes. Although univariate statistical approaches are often applied, we demonstrate here that the application of multivariate statistical approaches is highly recommended to fully capture the complexity of data gained using high-throughput methods. Methods We took blood plasma samples from 172 subjects who participated in the prospective Metabolic Syndrome Berlin Potsdam follow-up study (MESY-BEPO Follow-up). We analysed these samples using Gas Chromatography coupled with Mass Spectrometry (GC-MS), and measured 286 metabolites. Furthermore, fasting glucose levels were measured using standard methods at baseline, and after an average of six years. We did correlation analysis and built linear regression models as well as Random Forest regression models to identify metabolites that predict the development of fasting glucose in our cohort. Results We found a metabolic pattern consisting of nine metabolites that predicted fasting glucose development with an accuracy of 0.47 in tenfold cross-validation using Random Forest regression. We also showed that adding established risk markers did not improve the model accuracy. However, external validation is eventually desirable. Although not all metabolites belonging to the final pattern are identified yet, the pattern directs attention to amino acid metabolism, energy metabolism and redox homeostasis. Conclusions We demonstrate that metabolites identified using a high-throughput method (GC-MS) perform well in predicting the development of fasting plasma glucose over several years. Notably, not single, but a complex pattern of metabolites propels the prediction and therefore reflects the complexity of the underlying molecular mechanisms. This result could only be captured by application of multivariate statistical approaches. Therefore, we highly recommend the usage of statistical methods that seize the complexity of the information given by high-throughput methods.}, language = {en} } @misc{SakiMoazzenOberhaensli2012, author = {Saki, Adel and Moazzen, Mohssen and Oberh{\"a}nsli, Roland}, title = {Mineral chemistry and thermobarometry of the staurolite-chloritoid schists from Poshtuk, NW Iran}, series = {Postprints der Universit{\"a}t Potsdam : Mathematisch Naturwissenschaftliche Reihe}, journal = {Postprints der Universit{\"a}t Potsdam : Mathematisch Naturwissenschaftliche Reihe}, number = {578}, issn = {1866-8372}, doi = {10.25932/publishup-41394}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-413942}, pages = {1077 -- 1088}, year = {2012}, abstract = {The Poshtuk metapelitic rocks in northwestern Iran underwent two main phases of regional and contact metamorphism. Microstructures, textural features and field relations indicate that these rocks underwent a polymetamorphic history. The dominant metamorphic assemblage of the metapelites is garnet, staurolite, chloritoid, chlorite, muscovite and quartz, which grew mainly syntectonically during the later contact metamorphic event. Peak metamorphic conditions of this event took place at 580 ◦ C and ∼ 3-4 kbar, indicating that this event occurred under high-temperature and low-pressure conditions (HT/LP metamorphism), which reflects the high heat flow in this part of the crust. This event is mainly controlled by advective heat input through magmatic intrusions into all levels of the crust. These extensive Eocene metamorphic and magmatic activities can be associated with the early Alpine Orogeny, which resulted in this area from the convergence between the Arabian and Eurasian plates, and the Cenozoic closure of the Tethys oceanic tract(s).}, language = {en} } @misc{LarhlimiDavidSelbigetal.2012, author = {Larhlimi, Abdelhalim and David, Laszlo and Selbig, Joachim and Bockmayr, Alexander}, title = {F2C2}, series = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe}, journal = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe}, number = {921}, issn = {1866-8372}, doi = {10.25932/publishup-43243}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-432431}, pages = {11}, year = {2012}, abstract = {Background: Flux coupling analysis (FCA) has become a useful tool in the constraint-based analysis of genome-scale metabolic networks. FCA allows detecting dependencies between reaction fluxes of metabolic networks at steady-state. On the one hand, this can help in the curation of reconstructed metabolic networks by verifying whether the coupling between reactions is in agreement with the experimental findings. On the other hand, FCA can aid in defining intervention strategies to knock out target reactions. Results: We present a new method F2C2 for FCA, which is orders of magnitude faster than previous approaches. As a consequence, FCA of genome-scale metabolic networks can now be performed in a routine manner. Conclusions: We propose F2C2 as a fast tool for the computation of flux coupling in genome-scale metabolic networks. F2C2 is freely available for non-commercial use at https://sourceforge.net/projects/f2c2/files/.}, language = {en} } @misc{KruegelVituEngbert2012, author = {Kr{\"u}gel, Andr{\´e} and Vitu, Fran{\c{c}}oise and Engbert, Ralf}, title = {Fixation positions after skipping saccades}, series = {Postprints der Universit{\"a}t Potsdam : Mathematisch Naturwissenschaftliche Reihe}, journal = {Postprints der Universit{\"a}t Potsdam : Mathematisch Naturwissenschaftliche Reihe}, number = {856}, issn = {1866-8372}, doi = {10.25932/publishup-43288}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-432887}, pages = {1556 -- 1561}, year = {2012}, abstract = {During reading, saccadic eye movements are generated to shift words into the center of the visual field for lexical processing. Recently, Krugel and Engbert (Vision Research 50:1532-1539, 2010) demonstrated that within-word fixation positions are largely shifted to the left after skipped words. However, explanations of the origin of this effect cannot be drawn from normal reading data alone. Here we show that the large effect of skipped words on the distribution of within-word fixation positions is primarily based on rather subtle differences in the low-level visual information acquired before saccades. Using arrangements of "x" letter strings, we reproduced the effect of skipped character strings in a highly controlled single-saccade task. Our results demonstrate that the effect of skipped words in reading is the signature of a general visuomotor phenomenon. Moreover, our findings extend beyond the scope of the widely accepted range-error model, which posits that within-word fixation positions in reading depend solely on the distances of target words. We expect that our results will provide critical boundary conditions for the development of visuomotor models of saccade planning during reading.}, language = {en} }