@article{SchmalzlinvanDongenKlimantetal.2005, author = {Schmalzlin, E. and van Dongen, J. T. and Klimant, I. and Marmodee, Bettina and Steup, Martin and Fisahn, Joachim and Geigenberger, Peter Ludwig and L{\"o}hmannsr{\"o}ben, Hans-Gerd}, title = {An optical multifrequency phase-modulation method using microbeads for measuring intracellular oxygen concentrations in plants}, issn = {0006-3495}, year = {2005}, abstract = {A technique has been developed to measure absolute intracellular oxygen concentrations in green plants. Oxygen- sensitive phosphorescent microbeads were injected into the cells and an optical multifrequency phase-modulation technique was used to discriminate the sensor signal from the strong auto fluorescence of the plant tissue. The method was established using photosynthesis- competent cells of the giant algae Chara corallina L., and was validated by application to various cell types of other plant species}, language = {en} }