@misc{ZhangIgnatova2009, author = {Zhang, Gong and Ignatova, Zoya}, title = {Generic algorithm to predict the speed of translational elongation : implications for protein biogenesis}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-45007}, year = {2009}, abstract = {Synonymous codon usage and variations in the level of isoaccepting tRNAs exert a powerful selective force on translation fidelity. We have developed an algorithm to evaluate the relative rate of translation which allows large-scale comparisons of the non-uniform translation rate on the protein biogenesis. Using the complete genomes of Escherichia coli and Bacillus subtilis we show that stretches of codons pairing to minor tRNAs form putative sites to locally attenuate translation; thereby the tendency is to cluster in near proximity whereas long contiguous stretches of slow-translating triplets are avoided. The presence of slow-translating segments positively correlates with the protein length irrespective of the protein abundance. The slow-translating clusters are predominantly located down-stream of the domain boundaries presumably to fine-tune translational accuracy with the folding fidelity of multidomain proteins. Translation attenuation patterns at highly structurally and functionally conserved domains are preserved across the species suggesting a concerted selective pressure on the codon selection and species-specific tRNA abundance in these regions.}, language = {en} } @misc{ZghalColsonBlainetal.2019, author = {Zghal, Firas and Colson, Serge S. and Blain, Gr{\´e}gory and Behm, David George and Granacher, Urs and Chaouachi, Anis}, title = {Combined Resistance and Plyometric Training Is More Effective Than Plyometric Training Alone for Improving Physical Fitness of Pubertal Soccer Players}, series = {Postprints der Universit{\"a}t Potsdam Humanwissenschaftliche Reihe}, journal = {Postprints der Universit{\"a}t Potsdam Humanwissenschaftliche Reihe}, number = {576}, issn = {1866-8364}, doi = {10.25932/publishup-43781}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-437810}, pages = {12}, year = {2019}, abstract = {The purpose of this study was to compare the effects of combined resistance and plyometric/sprint training with plyometric/sprint training or typical soccer training alone on muscle strength and power, speed, change-of-direction ability in young soccer players. Thirty-one young (14.5 ± 0.52 years; tanner stage 3-4) soccer players were randomly assigned to either a combined- (COMB, n = 14), plyometric-training (PLYO, n = 9) or an active control group (CONT, n = 8). Two training sessions were added to the regular soccer training consisting of one session of light-load high-velocity resistance exercises combined with one session of plyometric/sprint training (COMB), two sessions of plyometric/sprint training (PLYO) or two soccer training sessions (CONT). Training volume was similar between the experimental groups. Before and after 7-weeks of training, peak torque, as well as absolute and relative (normalized to torque; RTDr) rate of torque development (RTD) during maximal voluntary isometric contraction of the knee extensors (KE) were monitored at time intervals from the onset of contraction to 200 ms. Jump height, sprinting speed at 5, 10, 20-m and change-of-direction ability performances were also assessed. There were no significant between-group baseline differences. Both COMB and PLYO significantly increased their jump height (Δ14.3\%; ES = 0.94; Δ12.1\%; ES = 0.54, respectively) and RTD at mid to late phases but with greater within effect sizes in COMB in comparison with PLYO. However, significant increases in peak torque (Δ16.9\%; p < 0.001; ES = 0.58), RTD (Δ44.3\%; ES = 0.71), RTDr (Δ27.3\%; ES = 0.62) and sprint performance at 5-m (Δ-4.7\%; p < 0.001; ES = 0.73) were found in COMB without any significant pre-to-post change in PLYO and CONT groups. Our results suggest that COMB is more effective than PLYO or CONT for enhancing strength, sprint and jump performances.}, language = {en} } @misc{ZeuschnerParpiievPezeriletal.2019, author = {Zeuschner, Steffen Peer and Parpiiev, Tymur and Pezeril, Thomas and Hillion, Arnaud and Dumesnil, Karine and Anane, Abdelmadjid and Pudell, Jan-Etienne and Willig, Lisa and R{\"o}ssle, Matthias and Herzog, Marc and Reppert, Alexander von and Bargheer, Matias}, title = {Tracking picosecond strain pulses in heterostructures that exhibit giant magnetostriction}, series = {Postprints der Universit{\"a}t Potsdam : Mathematisch-naturwissenschaftliche Reihe}, journal = {Postprints der Universit{\"a}t Potsdam : Mathematisch-naturwissenschaftliche Reihe}, number = {706}, issn = {1866-8372}, doi = {10.25932/publishup-42845}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-428457}, pages = {9}, year = {2019}, abstract = {We combine ultrafast X-ray diffraction (UXRD) and time-resolved Magneto-Optical Kerr Effect (MOKE) measurements to monitor the strain pulses in laser-excited TbFe2/Nb heterostructures. Spatial separation of the Nb detection layer from the laser excitation region allows for a background-free characterization of the laser-generated strain pulses. We clearly observe symmetric bipolar strain pulses if the excited TbFe2 surface terminates the sample and a decomposition of the strain wavepacket into an asymmetric bipolar and a unipolar pulse, if a SiO2 glass capping layer covers the excited TbFe2 layer. The inverse magnetostriction of the temporally separated unipolar strain pulses in this sample leads to a MOKE signal that linearly depends on the strain pulse amplitude measured through UXRD. Linear chain model simulations accurately predict the timing and shape of UXRD and MOKE signals that are caused by the strain reflections from multiple interfaces in the heterostructure.}, language = {en} } @misc{ZeuschnerMatternPudelletal.2021, author = {Zeuschner, Steffen Peer and Mattern, Maximilian and Pudell, Jan-Etienne and Reppert, Alexander von and R{\"o}ssle, Matthias and Leitenberger, Wolfram and Schwarzkopf, Jutta and Boschker, Jos and Herzog, Marc and Bargheer, Matias}, title = {Reciprocal space slicing}, series = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe}, journal = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe}, number = {1137}, issn = {1866-8372}, doi = {10.25932/publishup-49976}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-499761}, pages = {13}, year = {2021}, abstract = {An experimental technique that allows faster assessment of out-of-plane strain dynamics of thin film heterostructures via x-ray diffraction is presented. In contrast to conventional high-speed reciprocal space-mapping setups, our approach reduces the measurement time drastically due to a fixed measurement geometry with a position-sensitive detector. This means that neither the incident (ω) nor the exit (2θ) diffraction angle is scanned during the strain assessment via x-ray diffraction. Shifts of diffraction peaks on the fixed x-ray area detector originate from an out-of-plane strain within the sample. Quantitative strain assessment requires the determination of a factor relating the observed shift to the change in the reciprocal lattice vector. The factor depends only on the widths of the peak along certain directions in reciprocal space, the diffraction angle of the studied reflection, and the resolution of the instrumental setup. We provide a full theoretical explanation and exemplify the concept with picosecond strain dynamics of a thin layer of NbO2.}, language = {en} } @misc{ZennerBoettingerKonigorski2022, author = {Zenner, Alexander M. and B{\"o}ttinger, Erwin and Konigorski, Stefan}, title = {StudyMe}, series = {Zweitver{\"o}ffentlichungen der Universit{\"a}t Potsdam : Reihe der Digital Engineering Fakult{\"a}t}, journal = {Zweitver{\"o}ffentlichungen der Universit{\"a}t Potsdam : Reihe der Digital Engineering Fakult{\"a}t}, number = {18}, doi = {10.25932/publishup-58976}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-589763}, pages = {15}, year = {2022}, abstract = {N-of-1 trials are multi-crossover self-experiments that allow individuals to systematically evaluate the effect of interventions on their personal health goals. Although several tools for N-of-1 trials exist, there is a gap in supporting non-experts in conducting their own user-centric trials. In this study, we present StudyMe, an open-source mobile application that is freely available from https://play.google.com/store/apps/details?id=health.studyu.me and offers users flexibility and guidance in configuring every component of their trials. We also present research that informed the development of StudyMe, focusing on trial creation. Through an initial survey with 272 participants, we learned that individuals are interested in a variety of personal health aspects and have unique ideas on how to improve them. In an iterative, user-centered development process with intermediate user tests, we developed StudyMe that features an educational part to communicate N-of-1 trial concepts. A final empirical evaluation of StudyMe showed that all participants were able to create their own trials successfully using StudyMe and the app achieved a very good usability rating. Our findings suggest that StudyMe provides a significant step towards enabling individuals to apply a systematic science-oriented approach to personalize health-related interventions and behavior modifications in their everyday lives.}, language = {en} } @misc{ZemellaThoringHoffmeisteretal.2018, author = {Zemella, Anne and Thoring, Lena and Hoffmeister, Christian and Šamal{\´i}kov{\´a}, M{\´a}ria and Ehren, Patricia and W{\"u}stenhagen, Doreen Anja and Kubick, Stefan}, title = {Cell-free protein synthesis as a novel tool for directed glycoengineering of active erythropoietin}, series = {Postprints der Universit{\"a}t Potsdam : Mathematisch Naturwissenschaftliche Reihe}, journal = {Postprints der Universit{\"a}t Potsdam : Mathematisch Naturwissenschaftliche Reihe}, number = {824}, doi = {10.25932/publishup-42701}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-427017}, pages = {14}, year = {2018}, abstract = {As one of the most complex post-translational modification, glycosylation is widely involved in cell adhesion, cell proliferation and immune response. Nevertheless glycoproteins with an identical polypeptide backbone mostly differ in their glycosylation patterns. Due to this heterogeneity, the mapping of different glycosylation patterns to their associated function is nearly impossible. In the last years, glycoengineering tools including cell line engineering, chemoenzymatic remodeling and site-specific glycosylation have attracted increasing interest. The therapeutic hormone erythropoietin (EPO) has been investigated in particular by various groups to establish a production process resulting in a defined glycosylation pattern. However commercially available recombinant human EPO shows batch-to-batch variations in its glycoforms. Therefore we present an alternative method for the synthesis of active glycosylated EPO with an engineered O-glycosylation site by combining eukaryotic cell-free protein synthesis and site-directed incorporation of non-canonical amino acids with subsequent chemoselective modifications.}, language = {en} } @misc{ZehbeKolloscheLardongetal.2017, author = {Zehbe, Kerstin and Kollosche, Matthias and Lardong, Sebastian and Kelling, Alexandra and Schilde, Uwe and Taubert, Andreas}, title = {Ionogels based on poly(methyl methacrylate) and metal-containing ionic liquids}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-400607}, pages = {16}, year = {2017}, abstract = {Ionogels (IGs) based on poly(methyl methacrylate) (PMMA) and the metal-containing ionic liquids (ILs) bis-1-butyl-3-methlimidazolium tetrachloridocuprate(II), tetrachloride cobaltate(II), and tetrachlorido manganate(II) have been synthesized and their mechanical and electrical properties have been correlated with their microstructure. Unlike many previous examples, the current IGs show a decreasing stability in stress-strain experiments on increasing IL fractions. The conductivities of the current IGs are lower than those observed in similar examples in the literature. Both effects are caused by a two-phase structure with micrometer-sized IL-rich domains homogeneously dispersed an IL-deficient continuous PMMA phase. This study demonstrates that the IL-polymer miscibility and the morphology of the IGs are key parameters to control the (macroscopic) properties of IGs.}, language = {en} } @misc{ZeedenObrehtVeresetal.2020, author = {Zeeden, Christian and Obreht, Igor and Veres, Daniel and Kaboth-Bahr, Stefanie and Hošek, Jan and Marković, Slobodan B. and B{\"o}sken, Janina and Lehmkuhl, Frank and Rolf, Christian and Hambach, Ulrich}, title = {Smoothed millennial-scale palaeoclimatic reference data as unconventional comparison targets: Application to European loess records}, series = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe}, journal = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe}, number = {1}, issn = {1866-8372}, doi = {10.25932/publishup-52427}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-524271}, pages = {15}, year = {2020}, abstract = {Millennial-scale palaeoclimate variability has been documented in various terrestrial and marine palaeoclimate proxy records throughout the Northern Hemisphere for the last glacial cycle. Its clear expression and rapid shifts between different states of climate (Greenland Interstadials and Stadials) represents a correlation tool beyond the resolution of e.g. luminescence dating, especially relevant for terrestrial deposits. Usually, comparison of terrestrial proxy datasets and the Greenland ice cores indicates a complex expression of millennial-scale climate variability as recorded in terrestrial geoarchives including loess. Loess is the most widespread terrestrial geoarchive of the Quaternary and especially widespread over Eurasia. However, loess often records a smoothed representation of millennial-scale variability without all fidelity when compared to the Greenland data, this being a relevant limiting feature in integrating loess with other palaeoclimate records. To better understand the loess proxy-response to millennial-scale climate variability, we simulate a proxy signal smoothing by natural processes through application of low-pass filters of delta O-18 data from Greenland, a high-resolution palaeoclimate reference record, alongside speleothem isotope records from the Black Sea-Mediterranean region. We show that low-pass filters represent rather simple models for better constraining the expression of millennial-scale climate variability in low sedimentation environments, and in sediments where proxy-response signals are most likely affected by natural smoothing (by e.g. bioturbation). Interestingly, smoothed datasets from Greenland and the Black Sea-Mediterranean region are most similar in the last similar to 15 ka and between similar to 50-30 ka. Between similar to 30-15 ka, roughly corresponding to the Last Glacial Maximum and the deglaciation, the records show dissimilarities, challenging the construction of robust correlative time-scales in this age range. From our analysis it becomes apparent that patterns of palaeoclimate signals in loess-palaeosol sequences often might be better explained by smoothed Greenland reference data than the original high-resolution Greenland dataset, or other reference data. This opens the possibility to better assess the temporal resolution and palaeoclimate potential of loess-palaeosol sequences in recording supra-regional climate patterns, as well as to securely integrate loess with other chronologically better-resolved palaeoclimate records.}, language = {en} } @misc{ZechSchuchPerezChaparroetal.2020, author = {Zech, Philipp and Schuch, Felipe and P{\´e}rez Chaparro, Camilo Germ{\´a}n Alberto and Kangas, Maria and Rapp, Michael A. and Heissel, Andreas}, title = {Exercise, Comorbidities, and Health-Related Quality of Life in People Living with HIV}, series = {Postprints der Universit{\"a}t Potsdam : Humanwissenschaftliche Reihe}, journal = {Postprints der Universit{\"a}t Potsdam : Humanwissenschaftliche Reihe}, number = {661}, issn = {1866-8364}, doi = {10.25932/publishup-48028}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-480289}, pages = {15}, year = {2020}, abstract = {(1) Background: People with HIV (PWH) may perform more than one type of exercise cumulatively. The objective of this study is to investigate recreational exercise and its association with health-related quality of life (HRQOL) and comorbidities in relation to potential covariates. (2) Methods: The HIBES study (HIV-Begleiterkrankungen-Sport) is a cross-sectional study for people with HIV. The differences between non-exercisers versus exercisers (cumulated vs. single type of exercises) were investigated using regression models based on 454 participants. (3) Results: Exercisers showed a higher HRQOL score compared to non-exercisers (Wilcox r = 0.2 to 0.239). Psychological disorders were identified as the main covariate. Participants performing exercise cumulatively showed higher scores in duration, frequency, and intensity when compared to participants performing only one type of exercise. The mental health summary score was higher for the cumulated and single type of exercise if a psychological disorder existed. Duration and intensity were associated with an increase of HRQOL, whilst a stronger association between psychological disorders and exercise variables were evident. Exercise duration (minutes) showed a significant effect on QOL (standardized beta = 0.1) and for participants with psychological disorders (standardized beta = 0.3), respectively. (4) Conclusions: Psychological disorders and other covariates have a prominent effect on HRQOL and its association with exercise. For PWH with a psychological disorder, a stronger relationship between HRQOL with exercise duration and intensity emerged. However, differentiation of high-HRQOL individuals warrants further investigation by considering additional factors.}, language = {en} } @misc{ZarubaWestphalGutmannetal.2021, author = {Zaruba, Nicole and Westphal, Andrea and Gutmann, Franziska and Vock, Miriam}, title = {Preservice Teachers' Implicit and Explicit Attitudes Towards Teaching and Learning}, series = {Postprints der Universit{\"a}t Potsdam : Humanwissenschaftliche Reihe}, journal = {Postprints der Universit{\"a}t Potsdam : Humanwissenschaftliche Reihe}, issn = {1866-8364}, doi = {10.25932/publishup-54289}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-542895}, pages = {16}, year = {2021}, abstract = {When it comes to teacher attitudes towards teaching and learning, research relies heavily on explicit measures (e.g., questionnaires). These attitudes are generally conceptualized as constructivist and transmissive views on teaching and learning with constructivism often considered to be more desirable. In explicit measures, this can have drawbacks like socially desirable responding. It is for this reason that, in this study, we investigated implicit attitudes as well as explicit attitudes towards constructivism and transmission. N = 100 preservice teachers worked on a questionnaire and two Single-Target Implicit Association Tests (ST-IAT constructivism and ST-IAT transmission) before (T1) and after (T2) a single master's semester. One group (n = 50) did student teaching while a second group (n = 50) took master's courses. We evaluated preservice teachers' views on teaching at the end of their masters' studies. Participants agreed with transmission and constructivism (T1) on both an explicit and implicit level. Implicit measures seem to exceed explicit measures in differentially assessing constructivist and transmissive views on teaching and learning. After student teaching (T2), there was no overall effect of attitude development but changes in rank indicate that participants' implicit attitudes towards constructivism and transmission developed differently for each individual.}, language = {en} }