@article{GendreBaralDangetal.2019,
  author    = {Gendre, Delphine and Baral, Anirban and Dang, Xie and Esnay, Nicolas and Boutte, Yohann and Stanislas, Thomas and Vain, Thomas and Claverol, Stephane and Gustavsson, Anna and Lin, Deshu and Grebe, Markus and Bhalerao, Rishikesh P.},
  title     = {Rho-of-plant activated root hair formation requires Arabidopsis YIP4a/b gene function},
  series = {Development : Company of Biologists},
  volume    = {146},
  journal   = {Development : Company of Biologists},
  number    = {5},
  publisher = {The Company of Biologists},
  address   = {Cambridge},
  issn      = {0950-1991},
  doi       = {10.1242/dev.168559},
  pages     = {7},
  year      = {2019},
  abstract  = {Root hairs are protrusions from root epidermal cells with crucial roles in plant soil interactions. Although much is known about patterning, polarity and tip growth of root hairs, contributions of membrane trafficking to hair initiation remain poorly understood. Here, we demonstrate that the trans-Golgi network-localized YPT-INTERACTING PROTEIN 4a and YPT-INTERACTING PROTEIN 4b (YIP4a/b) contribute to activation and plasma membrane accumulation of Rho-of-plant (ROP) small GTPases during hair initiation, identifying YIP4a/b as central trafficking components in ROP-dependent root hair formation.},
  language  = {en}
}
@article{KieferClaesNzayisengaetal.2015,
  author    = {Kiefer, Christian S. and Claes, Andrea R. and Nzayisenga, Jean-Claude and Pietra, Stefano and Stanislas, Thomas and Ikeda, Yoshihisa and Grebe, Markus},
  title     = {Arabidopsis AIP1-2 restricted by WER-mediated patterning modulates planar polarity},
  series = {Development},
  journal   = {Development},
  number    = {142},
  doi       = {doi: 10.1242/dev.111013},
  pages     = {151 -- 161},
  year      = {2015},
  abstract  = {The coordination of cell polarity within the plane of the tissue layer (planar polarity) is crucial for the development of diverse multicellular organisms. Small Rac/Rho-family GTPases and the actin cytoskeleton contribute to planar polarity formation at sites of polarity establishment in animals and plants. Yet, upstream pathways coordinating planar polarity differ strikingly between kingdoms. In the root of Arabidopsis thaliana, a concentration gradient of the phytohormone auxin coordinates polar recruitment of Rho-of-plant (ROP) to sites of polar epidermal hair initiation. However, little is known about cytoskeletal components and interactions that contribute to this planar polarity or about their relation to the patterning machinery. Here, we show that ACTIN7 (ACT7) represents a main actin isoform required for planar polarity of root hair positioning, interacting with the negative modulator ACTIN-INTERACTING PROTEIN1-2 (AIP1-2). ACT7, AIP1-2 and their genetic interaction are required for coordinated planar polarity of ROP downstream of ethylene signalling. Strikingly, AIP1-2 displays hair cell file-enriched expression, restricted by WEREWOLF (WER)-dependent patterning and modified by ethylene and auxin action. Hence, our findings reveal AIP1-2, expressed under control of the WER-dependent patterning machinery and the ethylene signalling pathway, as a modulator of actin-mediated planar polarity.},
  language  = {en}
}
@article{StanislasHuserBarbosaetal.2015,
  author    = {Stanislas, Thomas and Huser, Anke and Barbosa, Ines C. R. and Kiefer, Christian S. and Brackmann, Klaus and Pietra, Stefano and Gustavsson, Anna and Zourelidou, Melina and Schwechheimer, Claus and Grebe, Markus},
  title     = {Arabidopsis D6PK is a lipid domain-dependent mediator of root epidermal planar polarity},
  series = {Nature plants},
  volume    = {1},
  journal   = {Nature plants},
  number    = {11},
  publisher = {Nature Publ. Group},
  address   = {London},
  issn      = {2055-026X},
  doi       = {10.1038/NPLANTS.2015.162},
  pages     = {9},
  year      = {2015},
  abstract  = {Development of diverse multicellular organisms relies on coordination of single-cell polarities within the plane of the tissue layer (planar polarity). Cell polarity often involves plasma membrane heterogeneity generated by accumulation of specific lipids and proteins into membrane subdomains. Coordinated hair positioning along Arabidopsis root epidermal cells provides a planar polarity model in plants, but knowledge about the functions of proteo-lipid domains in planar polarity signalling remains limited. Here we show that Rho-of-plant (ROP) 2 and 6, phosphatidylinositol-4-phosphate 5-kinase 3 (PIP5K3), DYNAMIN-RELATED PROTEIN (DRP) 1A and DRP2B accumulate in a sterol-enriched, polar membrane domain during root hair initiation. DRP1A, DRP2B, PIP5K3 and sterols are required for planar polarity and the AGCVIII kinase D6 PROTEIN KINASE (D6PK) is a modulator of this process. D6PK undergoes phosphatidylinositol-4,5-bisphosphate- and sterol-dependent basal-to-planar polarity switching into the polar, lipid-enriched domain just before hair formation, unravelling lipid-dependent D6PK localization during late planar polarity signalling.},
  language  = {en}
}
@article{KieferClaesNzayisengaetal.2015,
  author    = {Kiefer, Christian S. and Claes, Andrea R. and Nzayisenga, Jean-Claude and Pietra, Stefano and Stanislas, Thomas and Hueser, Anke and Ikeda, Yoshihisa and Grebe, Markus},
  title     = {Arabidopsis AIP1-2 restricted by WER-mediated patterning modulates planar polarity},
  series = {Development : Company of Biologists},
  volume    = {142},
  journal   = {Development : Company of Biologists},
  number    = {1},
  publisher = {Company of Biologists Limited},
  address   = {Cambridge},
  issn      = {0950-1991},
  doi       = {10.1242/dev.111013},
  pages     = {151 -- 161},
  year      = {2015},
  abstract  = {The coordination of cell polarity within the plane of the tissue layer (planar polarity) is crucial for the development of diverse multicellular organisms. Small Rac/Rho-family GTPases and the actin cytoskeleton contribute to planar polarity formation at sites of polarity establishment in animals and plants. Yet, upstream pathways coordinating planar polarity differ strikingly between kingdoms. In the root of Arabidopsis thaliana, a concentration gradient of the phytohormone auxin coordinates polar recruitment of Rho-of-plant (ROP) to sites of polar epidermal hair initiation. However, little is known about cytoskeletal components and interactions that contribute to this planar polarity or about their relation to the patterning machinery. Here, we show that ACTIN7 (ACT7) represents a main actin isoform required for planar polarity of root hair positioning, interacting with the negative modulator ACTIN-INTERACTING PROTEIN1-2 (AIP1-2). ACT7, AIP1-2 and their genetic interaction are required for coordinated planar polarity of ROP downstream of ethylene signalling. Strikingly, AIP1-2 displays hair cell file-enriched expression, restricted by WEREWOLF (WER)-dependent patterning and modified by ethylene and auxin action. Hence, our findings reveal AIP1-2, expressed under control of the WER-dependent patterning machinery and the ethylene signalling pathway, as a modulator of actin-mediated planar polarity.},
  language  = {en}
}
@article{FrescatadaRosaStanislasBackuesetal.2014,
  author    = {Frescatada-Rosa, Marcia and Stanislas, Thomas and Backues, Steven K. and Reichardt, Ilka and Men, Shuzhen and Boutte, Yohann and Juergens, Gerd and Moritz, Thomas and Bednarek, Sebastian York and Grebe, Markus},
  title     = {High lipid order of Arabidopsis cell-plate membranes mediated by sterol and Dynamin-Related Protein 1A function},
  series = {The plant journal},
  volume    = {80},
  journal   = {The plant journal},
  number    = {5},
  publisher = {Wiley-Blackwell},
  address   = {Hoboken},
  issn      = {0960-7412},
  doi       = {10.1111/tpj.12674},
  pages     = {745 -- 757},
  year      = {2014},
  abstract  = {Membranes of eukaryotic cells contain high lipid-order sterol-rich domains that are thought to mediate temporal and spatial organization of cellular processes. Sterols are crucial for execution of cytokinesis, the last stage of cell division, in diverse eukaryotes. The cell plate of higher-plant cells is the membrane structure that separates daughter cells during somatic cytokinesis. Cell-plate formation in Arabidopsis relies on sterol- and DYNAMIN-RELATED PROTEIN1A (DRP1A)-dependent endocytosis. However, functional relationships between lipid membrane order or lipid packing and endocytic machinery components during eukaryotic cytokinesis have not been elucidated. Using ratiometric live imaging of lipid order-sensitive fluorescent probes, we show that the cell plate of Arabidopsis thaliana represents a dynamic, high lipid-order membrane domain. The cell-plate lipid order was found to be sensitive to pharmacological and genetic alterations of sterol composition. Sterols co-localize with DRP1A at the cell plate, and DRP1A accumulates in detergent-resistant membrane fractions. Modifications of sterol concentration or composition reduce cell-plate membrane order and affect DRP1A localization. Strikingly, DRP1A function itself is essential for high lipid order at the cell plate. Our findings provide evidence that the cell plate represents a high lipid-order domain, and pave the way to explore potential feedback between lipid order and function of dynamin-related proteins during cytokinesis.},
  language  = {en}
}