@article{PecherHarnackGuntheretal.2001,
  author    = {Pecher, Gabriele and Harnack, U. and Gunther, M. and Hummel, M. and Fichtner, I. and Schenk, J{\"o}rg A.},
  title     = {Generation of an immortalized human CD4+ T cell clone inhibiting tumor growth in mice.},
  year      = {2001},
  abstract  = {Tumor antigen-specific T cell clones represent a useful tool in tumor immunology; however, their long-term culture is limited. To generate an immortalized cytotoxic T cell clone against the human tumor antigen mucin, we exposed a previously generated T cell culture to Herpesvirus saimiri. We obtained an immortalized human CD4+ T cell clone, termed SITAM. Clonality of these cells was shown by analysis of the alpha/beta-T cell receptor (TCR) repertoire. Cytolytic activity was demonstrated against several mucin-expressing tumor cell lines and could not be detected against non-mucin-expressing cells. SITAM cells maintained their features stably for 2 years. Furthermore, growth of the tumor cell line Capan-2 in NOD/SCID mice was inhibited when SITAM cells were coinjected subcutaneously with tumor cells. SITAM cells provide an unlimited source of clonal T cells for analysis of tumor recognition and may be of help in TCR-targeted immunotherapy.},
  language  = {en}
}