@article{StangeHintscheSachseetal.2017,
  author    = {Stange, Maike and Hintsche, Marius and Sachse, Kirsten and Gerhardt, Matthias and Valleriani, Angelo and Beta, Carsten},
  title     = {Analyzing the spatial positioning of nuclei in polynuclear giant cells},
  series = {Journal of Physics D: Applied Physics},
  volume    = {50},
  journal   = {Journal of Physics D: Applied Physics},
  number    = {46},
  publisher = {IOP Publ. Ltd.},
  address   = {Bristol},
  issn      = {0022-3727},
  doi       = {10.1088/1361-6463/aa8da0},
  pages     = {8},
  year      = {2017},
  abstract  = {How cells establish and maintain a well-defined size is a fundamental question of cell biology. Here we investigated to what extent the microtubule cytoskeleton can set a predefined cell size, independent of an enclosing cell membrane. We used electropulse-induced cell fusion to form giant multinuclear cells of the social amoeba Dictyostelium discoideum. Based on dual-color confocal imaging of cells that expressed fluorescent markers for the cell nucleus and the microtubules, we determined the subcellular distributions of nuclei and centrosomes in the giant cells. Our two- and three-dimensional imaging results showed that the positions of nuclei in giant cells do not fall onto a regular lattice. However, a comparison with model predictions for random positioning showed that the subcellular arrangement of nuclei maintains a low but still detectable degree of ordering. This can be explained by the steric requirements of the microtubule cytoskeleton, as confirmed by the effect of a microtubule degrading drug.},
  language  = {en}
}
@article{RudolphMohrToetzkeKardjilovetal.2017,
  author    = {Rudolph-Mohr, Nicole and Toetzke, Christian and Kardjilov, Nikolay and Oswald, Sascha},
  title     = {Mapping water, oxygen, and pH dynamics in the rhizosphere of young maize roots},
  series = {Journal of plant nutrition and soil science = Zeitschrift f{\"u}r Pflanzenern{\"a}hrung und Bodenkunde},
  volume    = {180},
  journal   = {Journal of plant nutrition and soil science = Zeitschrift f{\"u}r Pflanzenern{\"a}hrung und Bodenkunde},
  publisher = {Wiley-VCH},
  address   = {Weinheim},
  issn      = {1436-8730},
  doi       = {10.1002/jpln.201600120},
  pages     = {336 -- 346},
  year      = {2017},
  abstract  = {Rhizosphere processes are highly dynamic in time and space and strongly depend on each other. Key factors influencing pH changes in the rhizosphere are root exudation, respiration, and nutrient supply, which are influenced by soil water content levels. In this study, we measured the real-time distribution of soil water, pH changes, and oxygen distribution in the rhizosphere of young maize plants using a recently developed imaging approach. Neutron radiography was used to capture the root system and soil water distribution, while fluorescence imaging was employed to map soil pH and soil oxygen changes. Germinated seeds of maize (Zea mays L.) were planted in glass rhizotrons equipped with pH and oxygen-sensitive sensor foils. After 20 d, the rhizotrons were wetted from the bottom and time-lapsed images via fluorescence and neutron imaging were taken during the subsequent day and night cycles for 5 d. We found higher water content and stronger acidification in the first 0.5 mm from the root surface compared to the bulk soil, which could be a consequence of root exudation. While lateral roots only slightly acidified their rhizosphere, crown roots induced stronger acidification of up to 1 pH unit. We observed changing oxygen patterns at different soil moisture conditions and increasing towards lateral as well as crown roots while extending laterally with ongoing water logging. Our work indicates that plants alter the rhizosphere pH and oxygen also depending on root type, which may indirectly arise also from differences in age and water content changes. The results presented here were possible only by combining different imaging techniques to examine profiles at the root-soil interface in a comprehensive way during wetting and drying.},
  language  = {en}
}