@article{LauxBierHoelzel2018, author = {Laux, Eva-Maria and Bier, Frank Fabian and H{\"o}lzel, Ralph}, title = {Dielectrophoretic Stretching of DNA}, series = {DNA Nanotechnology}, journal = {DNA Nanotechnology}, edition = {2}, publisher = {Humana Press Inc.}, address = {New York}, isbn = {978-1-4939-8582-1}, issn = {1064-3745}, doi = {10.1007/978-1-4939-8582-1_14}, pages = {199 -- 208}, year = {2018}, abstract = {The spatial control of DNA and of self-assembled DNA constructs is a prerequisite for the preparation of DNA-based nanostructures and microstructures and a useful tool for studies on single DNA molecules. Here we describe a protocol for the accumulation of dissolved lambda-DNA molecules between planar microelectrodes by the action of inhomogeneous radiofrequency electric fields. The resulting AC electrokinetic forces stretch the DNA molecules and align them parallel to the electric field. The electrode preparation from off-the-shelf electronic components is explained, and a detailed description of the electronic setup is given. The experimental procedure is controlled in real-time by fluorescence microscopy.}, language = {en} } @article{Sugita2012, author = {Sugita, Yuko}, title = {Minimal affect uptake in a pre-climax position of conversational "scary" stories}, series = {Journal of pragmatics : an interdisciplinary journal of language studies}, volume = {44}, journal = {Journal of pragmatics : an interdisciplinary journal of language studies}, number = {10}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0378-2166}, doi = {10.1016/j.pragma.2012.05.012}, pages = {1273 -- 1289}, year = {2012}, abstract = {Analyzing pre-climax positions of everyday affect-laden telling activities, this paper shows that particular facial expressions, such as raised eyebrows with an open mouth or jaw-dropping, are the devices which are preferably deployed by story recipients as a minimal uptake to display affiliation, especially in the case of "scary" stories. During the course of conversational storytelling, it is structurally necessary that recipients warrant the tellers speakership. At the same time, a particular affective display-not only in response to what has been said but also to what comes at the climax-appears to become relevant. Immediately prior to the climax, when the teller employs elaborate multimodal cues, the recipient's display of an "anticipatory affect" is made relevant. A particular type of affect signals the anticipation of what kind of climax is approaching. The present paper explores how story recipients accomplish this two-fold task, namely to display alignment with the speaker's role allocation and listenership on the one hand and affiliation on the other. The study argues that a minimal uptake is called for, requiring only a minimal slot in the flow of storytelling and facial expressions that are most likely to fit this slot.}, language = {en} }