@article{PetrovSchippersBeninaetal.2013,
  author    = {Petrov, Veselin and Schippers, Jos and Benina, Maria and Minkov, Ivan and M{\"u}ller-R{\"o}ber, Bernd and Gechev, Tsanko S.},
  title     = {In search for new players of the oxidative stress network by phenotyping an Arabidopsis T-DNA mutant collection on reactive oxygen species-eliciting chemicals},
  series = {Plant omics},
  volume    = {6},
  journal   = {Plant omics},
  number    = {1},
  publisher = {Southern Cross Publ.},
  address   = {Lismore},
  issn      = {1836-0661},
  pages     = {46 -- 54},
  year      = {2013},
  abstract  = {The ability of some chemical compounds to cause oxidative stress offers a fast and convenient way to study the responses of plants to reactive oxygen species (ROS). In order to unveil potential novel genetic players of the ROS-regulatory network, a population of similar to 2,000 randomly selected Arabidopsis thaliana T-DNA insertion mutants was screened for ROS sensitivity/resistance by growing seedlings on agar medium supplemented with stress-inducing concentrations of the superoxide-eliciting herbicide methyl viologen or the catalase inhibitor 3-amino-triazole. A semi-robotic setup was used to capture and analyze images of the chemically treated seedlings which helped interpret the screening results by providing quantitative information on seedling area and healthy-to-chlorotic tissue ratios for data verification. A ROS-related phenotype was confirmed in three of the initially selected 33 mutant candidates, which carry T-DNA insertions in genes encoding a Ring/Ubox superfamily protein, ABI5 binding protein 1 (AFP1), previously reported to be involved in ABA signaling, and a protein of unknown function, respectively. In addition, we identified six mutants, most of which have not been described yet, that are related to growth or chloroplast development and show defects in a ROS-independent manner. Thus, semi-automated image capturing and phenotyping applied on publically available T-DNA insertion collections adds a simple means for discovering novel mutants in complex physiological processes and identifying the genes involved.},
  language  = {en}
}
@article{LissoSchroederMuessig2013,
  author    = {Lisso, Janina and Schr{\"o}der, Florian and M{\"u}ssig, Carsten},
  title     = {EXO modifies sucrose and trehalose responses and connects the extracellular carbon status to growth},
  series = {Frontiers in plant science},
  volume    = {4},
  journal   = {Frontiers in plant science},
  number    = {25},
  publisher = {Frontiers Research Foundation},
  address   = {Lausanne},
  issn      = {1664-462X},
  doi       = {10.3389/fpls.2013.00219},
  pages     = {18},
  year      = {2013},
  abstract  = {Plants have the capacity to adapt growth to changing environmental conditions. This implies the modulation of metabolism according to the availability of carbon (C). Particular interest in the response to the C availability is based on the increasing atmospheric levels of CO2. Several regulatory pathways that link the C status to growth have emerged. The extracellular EXO protein is essential for cell expansion and promotes shoot and root growth. Homologous proteins were identified in evolutionarily distant green plants. We show here that the EXO protein connects growth with C responses. The exo mutant displayed altered responses to exogenous sucrose supplemented to the growth medium. Impaired growth of the mutant in synthetic medium was associated with the accumulation of starch and anthocyanins, altered expression of sugar-responsive genes, and increased abscisic acid levels. Thus, EXO modulates several responses related to the C availability. Growth retardation on medium supplemented with 2-deoxy-glucose, mannose, and palatinose was similar to the wildtype. Trehalose feeding stimulated root growth and shoot biomass production of exoplants where as it inhibited growth of the wildtype. The phenotypic features of the exo mutant suggest that apoplastic processes coordinate growth and C responses.},
  language  = {en}
}