@article{SamahaHamdoCongetal.2020,
  author    = {Samaha, Doaa and Hamdo, Housam H. and Cong, Xiaojing and Schumacher, Fabian and Banhart, Sebastian and Aglar, {\"O}znur and M{\"o}ller, Heiko Michael and Heuer, Dagmar and Kleuser, Burkhard and Saied, Essa M. and Arenz, Christoph},
  title     = {Liposomal FRET assay identifies potent drug-like inhibitors of the Ceramide Transport Protein (CERT)},
  series = {Chemistry - a European journal},
  volume    = {26},
  journal   = {Chemistry - a European journal},
  number    = {70},
  publisher = {Wiley-VCH},
  address   = {Weinheim},
  issn      = {0947-6539},
  doi       = {10.1002/chem.202003283},
  pages     = {16616 -- 16621},
  year      = {2020},
  abstract  = {Ceramide transfer protein (CERT) mediates non-vesicular transfer of ceramide from endoplasmic reticulum to Golgi apparatus and thus catalyzes the rate-limiting step of sphingomyelin biosynthesis. Usually, CERT ligands are evaluated in tedious binding assays or non-homogenous transfer assays using radiolabeled ceramides. Herein, a facile and sensitive assay for CERT, based on Forster resonance energy transfer (FRET), is presented. To this end, we mixed donor and acceptor vesicles, each containing a different fluorescent ceramide species. By CERT-mediated transfer of fluorescent ceramide, a FRET system was established, which allows readout in 96-well plate format, despite the high hydrophobicity of the components. Screening of a 2 000 compound library resulted in two new potent CERT inhibitors. One is approved for use in humans and one is approved for use in animals. Evaluation of cellular activity by quantitative mass spectrometry and confocal microscopy showed inhibition of ceramide trafficking and sphingomyelin biosynthesis.},
  language  = {en}
}
@article{TechenCzaplaMoellnitzetal.2013,
  author    = {Techen, Anne and Czapla, Sylvia and M{\"o}llnitz, Kristian and Budach, Dennis B. and Wessig, Pablo and Kumke, Michael Uwe},
  title     = {Synthesis and spectroscopic characterization of fluorophore-labeled oligospiroketal rods},
  series = {Helvetica chimica acta},
  volume    = {96},
  journal   = {Helvetica chimica acta},
  number    = {11},
  publisher = {Wiley-VCH},
  address   = {Weinheim},
  issn      = {0018-019X},
  doi       = {10.1002/hlca.201200616},
  pages     = {2046 -- 2067},
  year      = {2013},
  abstract  = {Fluorescence probes consisting of well-established fluorophores in combination with rigid molecular rods based on spirane-type structures were investigated with respect to their fluorescence properties under different solvent conditions. The attachment of the dyes was accomplished by 1,3-dipolar cycloaddition between alkynes and azides (click' reaction) and is a prime example for a novel class of sensor constructs. Especially, the attachment of two (different) fluorophores on opposite sides of the molecular rods paves the way to new sensor systems with less bulky (compared to the conventional DNA- or protein-based concepts), nevertheless rigid spacer constructs, e.g., for FRET-based sensing applications. A detailed photophysical characterization was performed in MeOH (and in basic H2O/MeOH mixtures) for i) rod constructs containing carboxyfluorescein, ii) rod constructs containing carboxyrhodamine, iii) rod constructs containing both carboxyfluorescein and carboxyrhodamine, and iv) rod constructs containing both pyrene and perylene parts. For each dye (pair), two rod lengths with different numbers of spirane units were synthesized and investigated. The rod constructs were characterized in ensemble as well as single-molecule fluorescence experiments with respect to i) specific roddye and ii) dyedye interactions. In addition to MeOH and MeOH/NaOH, the rod constructs were also investigated in micellar systems, which were chosen as a simplified model for membranes.},
  language  = {en}
}