@article{AndresDelgadoErnstGalardiCastillaetal.2019,
  author    = {Andr{\´e}s-Delgado, Laura and Ernst, Alexander and Galardi-Castilla, Mar{\´i}a and Bazaga, David and Peralta, Marina and M{\"u}nch, Juliane and Gonzalez-Rosa, Juan M. and Marques, In{\^e}s and Tessadori, Federico and de la Pompa, Jos{\´e} Luis and Vermot, Julien and Mercader, Nadia},
  title     = {Actin dynamics and the Bmp pathway drive apical extrusion of proepicardial cells},
  series = {Development : Company of Biologists},
  volume    = {146},
  journal   = {Development : Company of Biologists},
  number    = {13},
  publisher = {The Company of Biologists Ltd},
  address   = {Cambridge},
  issn      = {0950-1991},
  doi       = {10.1242/dev.174961},
  pages     = {15},
  year      = {2019},
  abstract  = {The epicardium, the outer mesothelial layer enclosing the myocardium, plays key roles in heart development and regeneration. During embryogenesis, the epicardium arises from the proepicardium (PE), a cell cluster that appears in the dorsal pericardium (DP) close to the venous pole of the heart. Little is known about how the PE emerges from the pericardial mesothelium. Using a zebrafish model and a combination of genetic tools, pharmacological agents and quantitative in vivo imaging, we reveal that a coordinated collective movement of DP cells drives PE formation. We found that Bmp signaling and the actomyosin cytoskeleton promote constriction of the DP, which enables PE cells to extrude apically. We provide evidence that cell extrusion, which has been described in the elimination of unfit cells from epithelia and the emergence of hematopoietic stem cells, is also a mechanism for PE cells to exit an organized mesothelium and fulfil their developmental fate to form a new tissue layer, the epicardium.},
  language  = {en}
}
@article{LombardoHeiseMoghtadaeietal.2019,
  author    = {Lombardo, Ver{\´o}nica A. and Heise, Melina and Moghtadaei, Motahareh and Bornhorst, Dorothee and M{\"a}nner, J{\"o}rg and Abdelilah-Seyfried, Salim},
  title     = {Morphogenetic control of zebrafish cardiac looping by Bmp signaling},
  series = {Development : Company of Biologists},
  volume    = {146},
  journal   = {Development : Company of Biologists},
  number    = {22},
  publisher = {The Company of Biologists Ltd},
  address   = {Cambridge},
  issn      = {0950-1991},
  doi       = {10.1242/dev.180091},
  pages     = {13},
  year      = {2019},
  abstract  = {Cardiac looping is an essential and highly conserved morphogenetic process that places the different regions of the developing vertebrate heart tube into proximity of their final topographical positions. High-resolution 4D live imaging of mosaically labelled cardiomyocytes reveals distinct cardiomyocyte behaviors that contribute to the deformation of the entire heart tube. Cardiomyocytes acquire a conical cell shape, which is most pronounced at the superior wall of the atrioventricular canal and contributes to S-shaped bending. Torsional deformation close to the outflow tract contributes to a torque-like winding of the entire heart tube between its two poles. Anisotropic growth of cardiomyocytes based on their positions reinforces S-shaping of the heart. During cardiac looping, bone morphogenetic protein pathway signaling is strongest at the future superior wall of the atrioventricular canal. Upon pharmacological or genetic inhibition of bone morphogenetic protein signaling, myocardial cells at the superior wall of the atrioventricular canal maintain cuboidal cell shapes and S-shaped bending is impaired. This description of cellular rearrangements and cardiac looping regulation may also be relevant for understanding the etiology of human congenital heart defects.},
  language  = {en}
}
@misc{HaackAbdelilahSeyfried2016,
  author    = {Haack, Timm and Abdelilah-Seyfried, Salim},
  title     = {The force within: endocardial development, mechanotransduction and signalling during cardiac morphogenesis},
  series = {Development : Company of Biologists},
  volume    = {143},
  journal   = {Development : Company of Biologists},
  publisher = {Company of Biologists Limited},
  address   = {Cambridge},
  issn      = {0950-1991},
  doi       = {10.1242/dev.131425},
  pages     = {373 -- 386},
  year      = {2016},
  abstract  = {Endocardial cells are cardiac endothelial cells that line the interior of the heart tube. Historically, their contribution to cardiac development has mainly been considered from a morphological perspective. However, recent studies have begun to define novel instructive roles of the endocardium, as a sensor and signal transducer of biophysical forces induced by blood flow, and as an angiocrine signalling centre that is involved in myocardial cellular morphogenesis, regeneration and reprogramming. In this Review, we discuss how the endocardium develops, how endocardial-myocardial interactions influence the developing embryonic heart, and how the dysregulation of blood flowresponsive endocardial signalling can result in pathophysiological changes.},
  language  = {en}
}