@article{KovachKoetzFriberg2014, author = {Kovach, Ildyko and Koetz, Joachim and Friberg, Stig E.}, title = {Janus emulsions stabilized by phospholipids}, series = {Colloids and surfaces : an international journal devoted to the principles and applications of colloid and interface science ; A, Physicochemical and engineering aspects}, volume = {441}, journal = {Colloids and surfaces : an international journal devoted to the principles and applications of colloid and interface science ; A, Physicochemical and engineering aspects}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0927-7757}, doi = {10.1016/j.colsurfa.2013.08.065}, pages = {66 -- 71}, year = {2014}, abstract = {Janus emulsions were formed by mixing three immiscible liquids; this implies two oil components (i.e. olive oil (00) and silicone oil (SiO)) with water in presence of interfacial active components. The morphology and size of Janus droplets formed strongly depended on the type of surfactant used. In presence of a non-ionic surfactant, i.e. Tween 80, large engulfed Janus droplets were formed. By adding phospholipids to the system the droplet size was decreased and more stable Janus droplets formed. Interfacial tension measurements carried out using a spinning drop apparatus and a ring tensiometer demonstrate that interfacial tension is the most important factor controlling the size, morphology and stability of Janus droplets. When the interfacial tension between oil and water becomes <= 1 mN/m, smaller Janus droplets are formed. Such conditions are fulfilled when phospholipids are used in combination with non-ionic surfactant Tween 80. The morphology of the double droplets is predominantly controlled by the viscosity and interfacial tension between the two oil phases. By using different types of phospholipids, i.e. asolectin and lecithin instead of a more concentrated phosphatidylcholine (phospholipon), the interfacial tension is decreased and different morphologies of engulfing can be observed.}, language = {en} } @article{AuyyuenyongHenzeUngruetal.2017, author = {Auyyuenyong, Ratchada and Henze, Andrea and Ungru, Julia and Schweigert, Florian Johannes and Raila, Jens and Vervuert, Ingrid}, title = {Determination of lipid profiles in serum of obese ponies before and after weight reduction by using multi-one-dimensional thin-layer chromatography}, series = {Research in veterinary science}, volume = {117}, journal = {Research in veterinary science}, publisher = {Elsevier}, address = {Oxford}, issn = {0034-5288}, doi = {10.1016/j.rvsc.2017.11.013}, pages = {111 -- 117}, year = {2017}, abstract = {Obesity is a key component of equine metabolic syndrome, which is highly associated with laminitis. Feed restriction and/or exercise are known to alleviate the detrimental effects of insulin resistance in obese ponies. However, little is known about changes in the serum lipid patterns due to weight reduction and its association with disease outcomes. Therefore, the lipid patterns in the serum of 14 mature ponies before and after a 14-week body weight reduction program (BWRP) were investigated by multi-one-dimensional thin-layer chromatography (MOD-TLC). Additionally, sensitivity to insulin (SI), body condition scores (BCS) and cresty neck scores (CNS) were measured. A BWRP resulted in a significant loss of body weight (P < 0.001), which was associated with beneficial decreases in BCS and CNS (both, P < 0.001). Serum lipid compositions revealed significantly increased free fatty acid (FFA), sphingomyelin (SM; both P < 0.001), total cholesterol (C) and cholesterol ester (CE) (both P < 0.01) and triacylglycerol (TG; P < 0.05) densities. Improvement of SI after the BWRP was associated with increases in neutral lipids (C, CE and TG, all P < 0.01), FFA and the phospholipid SM (both, P < 0.001). The results show that a BWRP in obese ponies was effective and associated with changes in the concentrations of neutral lipids and the phospholipid SM, indicating that SM may play a role in insulin signaling pathways and thus in the pathogenesis of insulin resistance and the progression of metabolic syndrome in obese ponies.}, language = {en} }