@phdthesis{Jing2020, author = {Jing, Yue}, title = {Characterization of Serine Carboxypeptidase-like (SCPL) gene family in Brassicaceae}, school = {Universit{\"a}t Potsdam}, year = {2020}, language = {en} } @phdthesis{Lange2020, author = {Lange, Nils}, title = {Matthias Walden}, publisher = {be.bra}, address = {Berlin}, isbn = {978-3-95410-279-2}, school = {Universit{\"a}t Potsdam}, pages = {620}, year = {2020}, abstract = {Matthias Walden (1927-1984) war einer der Vertreter eines politischen Neuanfangs im Journalismus in Deutschland nach 1945. Im Kern seines politischen Denkens stand die Verteidigung der liberalen Demokratie, deren ideellen Gehalt Walden sowohl durch eine personelle Kontinuit{\"a}t in der Bundesrepublik Deutschland zum Nationalsozialismus als auch durch die von ihm als Anbiederung empfundene Neue Ostpolitik und den gesellschaftlichen Protest der 1960er und 1970er Jahre in Gefahr sah. Als profilierter Leitartikler wurde er vor allem f{\"u}r den Verlag Axel Springer zu einem intellektuellen Impulsgeber. Walden war {\"u}berzeugt, Diktaturen und totalit{\"a}re Gesellschaftsentw{\"u}rfe s{\"a}hen immer nur so aus, als w{\"a}ren sie f{\"u}r die Ewigkeit gemacht. Im Kalten Krieg gab ihm gerade die Unmenschlichkeit der kommunistischen Regime die Gewissheit, dass diese einst verschwinden w{\"u}rden. Nils Lange legt mit seiner intellektuellen Biographie von Matthias Walden die erste umfassende Arbeit {\"u}ber diesen streitbaren Journalisten vor. Er arbeitet sowohl die politischen als auch die ideengeschichtlichen Urspr{\"u}nge von Waldens Denken heraus.}, language = {de} } @phdthesis{Temmen2020, author = {Temmen, Jens}, title = {The Territorialities of U.S. Imperialism(s)}, series = {American Studies ; 308}, journal = {American Studies ; 308}, publisher = {Winter}, address = {Heidelberg}, isbn = {978-3-8253-4713-0}, school = {Universit{\"a}t Potsdam}, pages = {x, 259}, year = {2020}, abstract = {'The Territorialities of U.S. Imperialisms' sets into relation U.S. imperial and Indigenous conceptions of territoriality as articulated in U.S. legal texts and Indigenous life writing in the 19th century. It analyzes the ways in which U.S. legal texts as "legal fictions" narratively press to affirm the United States' territorial sovereignty and coherence in spite of its reliance on a variety of imperial practices that flexibly disconnect and (re)connect U.S. sovereignty, jurisdiction and territory. At the same time, the book acknowledges Indigenous life writing as legal texts in their own right and with full juridical force, which aim to highlight the heterogeneity of U.S. national territory both from their individual perspectives and in conversation with these legal fictions. Through this, the book's analysis contributes to a more nuanced understanding of the coloniality of U.S. legal fictions, while highlighting territoriality as a key concept in the fashioning of the narrative of U.S. imperialism.}, language = {en} } @phdthesis{Moratti2020, author = {Moratti, Fabio Giulio}, title = {Structural analysis of DYW proteins and identification of the mitochondrial DNA-binding proteome of Arabidopsis thaliana}, school = {Universit{\"a}t Potsdam}, pages = {164}, year = {2020}, language = {en} } @phdthesis{Wenk2020, author = {Wenk, Sebastian}, title = {Engineering formatotrophic growth in Escherichia coli}, school = {Universit{\"a}t Potsdam}, pages = {V, 107}, year = {2020}, abstract = {To meet the demands of a growing world population while reducing carbon dioxide (CO2) emissions, it is necessary to capture CO2 and convert it into value-added compounds. In recent years, metabolic engineering of microbes has gained strong momentum as a strategy for the production of valuable chemicals. As common microbial feedstocks like glucose directly compete with human consumption, the one carbon (C1) compound formate was suggested as an alternative feedstock. Formate can be easily produced by various means including electrochemical reduction of CO2 and could serve as a feedstock for microbial production, hence presenting a novel entry point for CO2 to the biosphere and a storage option for excess electricity. Compared to the gaseous molecule CO2, formate is a highly soluble compound that can be easily handled and stored. It can serve as a carbon and energy source for natural formatotrophs, but these microbes are difficult to cultivate and engineer. In this work, I present the results of several projects that aim to establish efficient formatotrophic growth of E. coli - which cannot naturally grow on formate - via synthetic formate assimilation pathways. In the first study, I establish a workflow for growth-coupled metabolic engineering of E. coli. I demonstrate this approach by presenting an engineering scheme for the PFL-threonine cycle, a synthetic pathway for anaerobic formate assimilation in E. coli. The described methods are intended to create a standardized toolbox for engineers that aim to establish novel metabolic routes in E. coli and related organisms. The second chapter presents a study on the catalytic efficiency of C1-oxidizing enzymes in vivo. As formatotrophic growth requires generation of both energy and biomass from formate, the engineered E. coli strains need to be equipped with a highly efficient formate dehydrogenase, which provides reduction equivalents and ATP for formate assimilation. I engineered a strain that cannot generate reducing power and energy for cellular growth, when fed on acetate. Under this condition, the strain depends on the introduction of an enzymatic system for NADH regeneration, which could further produce ATP via oxidative phosphorylation. I show that the strain presents a valuable testing platform for C1-oxidizing enzymes by testing different NAD-dependent formate and methanol dehydrogenases in the energy auxotroph strain. Using this platform, several candidate enzymes with high in vivo activity, were identified and characterized as potential energy-generating systems for synthetic formatotrophic or methylotrophic growth in E. coli.   In the third chapter, I present the establishment of the serine threonine cycle (STC) - a synthetic formate assimilation pathway - in E. coli. In this pathway, formate is assimilated via formate tetrahydrofolate ligase (FtfL) from Methylobacterium extorquens (M. extorquens). The carbon from formate is attached to glycine to produce serine, which is converted into pyruvate entering central metabolism. Via the natural threonine synthesis and cleavage route, glycine is regenerated and acetyl-CoA is produced as the pathway product. I engineered several selection strains that depend on different STC modules for growth and determined key enzymes that enable high flux through threonine synthesis and cleavage. I could show that expression of an auxiliary formate dehydrogenase was required to achieve growth via threonine synthesis and cleavage on pyruvate. By overexpressing most of the pathway enzymes from the genome, and applying adaptive laboratory evolution, growth on glycine and formate was achieved, indicating the activity of the complete cycle. The fourth chapter shows the establishment of the reductive glycine pathway (rGP) - a short, linear formate assimilation route - in E. coli. As in the STC, formate is assimilated via M. extorquens FtfL. The C1 from formate is condensed with CO2 via the reverse reaction of the glycine cleavage system to produce glycine. Another carbon from formate is attached to glycine to form serine, which is assimilated into central metabolism via pyruvate. The engineered E. coli strain, expressing most of the pathway genes from the genome, can grow via the rGP with formate or methanol as a sole carbon and energy source.}, language = {en} } @phdthesis{Diab2020, author = {Diab, Sarah}, title = {Der Einfluss von nachhaltigem Konsumbewusstsein auf Luxuskonsum unter Ber{\"u}cksichtigung verschiedener Moderatoren}, school = {Universit{\"a}t Potsdam}, pages = {259}, year = {2020}, abstract = {Das Ziel der vorliegenden Dissertation war es herauszuarbeiten, ob Nachhaltigkeitsbewusstsein den Konsum von Luxusg{\"u}tern beeinflusst und ob verschiedene Moderatoren einen Einfluss auf diesen Zusammenhang aus{\"u}ben. Das Nachhaltigkeitsbewusstsein wurde dabei basierend auf dem von Balderjahn et al. (2013) entwickelten Consciousness-for-sustainable-consumption-Modell durch die {\"o}kologische, soziale und die {\"o}konomische Nachhaltigkeit sowie erg{\"a}nzend durch das Tierschutzbewusstsein und Bewusstsein f{\"u}r lokale Produktion repr{\"a}sentiert. Als Moderatoren dienten das Streben nach sozialer Anerkennung und Prestige, Materialismus, Hedonismus und Traditionsbewusstsein. F{\"u}r die Aufdeckung m{\"o}glicher Zusammenh{\"a}nge zwischen den verschiedenen Dimensionen der Nachhaltigkeit und Luxuskonsum wurde eine Pr{\"a}diktorenanalyse durchgef{\"u}hrt. Moderatorenanalysen offenbarten zus{\"a}tzlich, ob ein Einfluss der verschiedenen Moderatoren auf die einzelnen Zusammenh{\"a}nge vorlag. Die Untersuchung zeigte, dass jeweils das Umweltbewusstsein, das Bewusstsein f{\"u}r gen{\"u}gsamen Konsum sowie das Bewusstsein f{\"u}r schuldenfreien Konsum als Teil der {\"o}konomischen Nachhaltigkeit und das Tierschutzbewusstsein einen Einfluss auf den Luxuskonsum aus{\"u}ben. Dar{\"u}ber hinaus konnten insgesamt sieben Einfl{\"u}sse durch die verschiedenen Moderatorvariablen auf die unterschiedlichen Zusammenh{\"a}nge zwischen den Nachhaltigkeitsdimensionen und Luxuskonsum aufgedeckt werden.}, language = {de} } @phdthesis{Kubis2020, author = {Kubis, Armin}, title = {Synthetic carbon neutral photorespiration bypasses}, school = {Universit{\"a}t Potsdam}, pages = {68}, year = {2020}, abstract = {With populations growing worldwide and climate change threatening food production there is an urgent need to find ways to ensure food security. Increasing carbon fixation rate in plants is a promising approach to boost crop yields. The carbon-fixing enzyme Rubisco catalyzes, beside the carboxylation reaction, also an oxygenation reaction that generates glycolate-2P, which needs to be recycled via a metabolic route termed photorespiration. Photorespiration dissipates energy and most importantly releases previously fixed CO2, thus significantly lowering carbon fixation rate and yield. Engineering plants to omit photorespiratory CO2 release is the goal of the FutureAgriculture consortium and this thesis is part of this collaboration. The consortium aims to establish alternative glycolate-2P recycling routes that do not release CO2. Ultimately, they are expected to increase carbon fixation rates and crop yields. Natural and novel reactions, which require enzyme engineering, were considered in the pathway design process. Here I describe the engineering of two pathways, the arabinose-5P and the erythrulose shunt. They were designed to recycle glycolate-2P via glycolaldehyde into a sugar phosphate and thereby reassimilate glycolate-2P to the Calvin cycle. I used Escherichia coli gene deletion strains to validate and characterize the activity of both synthetic shunts. The strains' auxotrophies can be alleviated by the activity of the synthetic route, thus providing a direct way to select for pathway activity. I introduced all pathway components to these dedicated selection strains and discovered inhibitions, limitations and metabolic cross talk interfering with pathway activity. After resolving these issues, I was able to show the in vivo activity of all pathway components and combine them into functional modules.. Specifically, I demonstrate the activity of a new-to-nature module of glycolate reduction to glycolaldehyde. Also, I successfully show a new glycolaldehyde assimilation route via arabinose-5P to ribulose-5P. In addition, all necessary enzymes for glycolaldehyde assimilation via L-erythrulose were shown to be active and an L-threitol assimilation route via L-erythrulose was established in E. coli. On their own, these findings demonstrate the power of using an easily engineerable microbe to test novel pathways; combined, they will form the basis for implementing photorespiration bypasses in plants.}, language = {en} } @phdthesis{Kožul2020, author = {Kožul, Danijela}, title = {Systematic identification of loci determining chloroplast and nuclear genome incompatibility in the evening primrose (Oenothera)}, school = {Universit{\"a}t Potsdam}, pages = {126}, year = {2020}, language = {en} } @phdthesis{Ziemann2020, author = {Ziemann, Vanessa}, title = {Toxische Effekte von Arsenolipiden in humanen Kulturzellen und Caenorhabditis elegans}, school = {Universit{\"a}t Potsdam}, pages = {112}, year = {2020}, language = {de} } @phdthesis{Lambrecht2020, author = {Lambrecht, Jennifer}, title = {Warum machen wir nicht einfach Inklusion?}, series = {Perspektive Schule}, journal = {Perspektive Schule}, number = {1}, publisher = {W. Bertelsmann Verlag}, address = {Bielefeld}, isbn = {978-3-7639-6117-7}, school = {Universit{\"a}t Potsdam}, pages = {236}, year = {2020}, abstract = {Die Entwicklung einer Theorie zur schulischen Inklusion ist das zentrale Thema der Dissertation. Die Autorin nutzt empirische Analysen zur Umsetzung inklusiven Lernens sowie Daten zu sonderp{\"a}dagogischen F{\"o}rderschwerpunkten an inklusiven Grundschulen f{\"u}r die Erarbeitung von Bedingungen und Formen eines inklusiven Schulsystems. Empirische Daten zur Umsetzung inklusiver Bildung liegen aus vielen Bundesl{\"a}ndern vor, es fehlte jedoch eine forschungsleitende Theorie zur Einordnung und Analyse der Daten. Jennifer Lambrecht hat diese Theorie auf Grundlage der Systemtheorie Luhmanns entwickelt. Sie differenziert zwischen Schulsystemen und verortet unterschiedliche Inklusionsverst{\"a}ndnisse. Im Ergebnis entwickelt sie f{\"u}nf Thesen zur schulischen Inklusion im allgemeinen Schulsystem und im Sonderschulsystem. Die Dissertation, die ein hochaktuelles Thema der empirischen Bildungsforschung behandelt, regt zum Mit- und Nachdenken an und generiert neue, interessante Forschungsfragen.}, language = {de} }