@article{PaoliniFontanaVanCuongPhametal.2021,
  author    = {Paolini, Alessio and Fontana, Federica and Van-Cuong Pham, and R{\"o}del, Claudia Jasmin and Seyfried, Salim},
  title     = {Mechanosensitive Notch-Dll4 and Klf2-Wnt9 signaling pathways intersect in guiding valvulogenesis in zebrafish},
  series = {Cell reports},
  volume    = {37},
  journal   = {Cell reports},
  number    = {1},
  publisher = {Cell Press},
  address   = {Maryland Heights, MO},
  issn      = {2211-1247},
  doi       = {10.1016/j.celrep.2021.109782},
  pages     = {13},
  year      = {2021},
  abstract  = {In the zebrafish embryo, the onset of blood flow generates fluid shear stress on endocardial cells, which are specialized endothelial cells that line the interior of the heart. High levels of fluid shear stress activate both Notch and Klf2 signaling, which play crucial roles in atrioventricular valvulogenesis. However, it remains unclear why only individual endocardial cells ingress into the cardiac jelly and initiate valvulogenesis. Here, we show that lateral inhibition between endocardial cells, mediated by Notch, singles out Delta-like-4-positive endocardial cells. These cells ingress into the cardiac jelly, where they form an abluminal cell population. Delta-like-4-positive cells ingress in response to Wnt9a, which is produced in parallel through an Erk5Klf2-Wnt9a signaling cascade also activated by blood flow. Hence, mechanical stimulation activates parallel mechanosensitive signaling pathways that produce binary effects by driving endocardial cells toward either luminal or abluminal fates. Ultimately, these cell fate decisions sculpt cardiac valve leaflets.},
  language  = {en}
}
@article{BornhorstSeyfried2021,
  author    = {Bornhorst, Dorothee and Seyfried, Salim},
  title     = {Strong as a hippo's heart},
  series = {Frontiers in cell and developmental biology},
  volume    = {9},
  journal   = {Frontiers in cell and developmental biology},
  publisher = {Frontiers Media},
  address   = {Lausanne, Schweiz},
  issn      = {2296-634X},
  doi       = {10.3389/fcell.2021.731101},
  pages     = {1 -- 10},
  year      = {2021},
  abstract  = {The heart is comprised of multiple tissues that contribute to its physiological functions. During development, the growth of myocardium and endocardium is coupled and morphogenetic processes within these separate tissue layers are integrated. Here, we discuss the roles of mechanosensitive Hippo signaling in growth and morphogenesis of the zebrafish heart. Hippo signaling is involved in defining numbers of cardiac progenitor cells derived from the secondary heart field, in restricting the growth of the epicardium, and in guiding trabeculation and outflow tract formation. Recent work also shows that myocardial chamber dimensions serve as a blueprint for Hippo signaling-dependent growth of the endocardium. Evidently, Hippo pathway components act at the crossroads of various signaling pathways involved in embryonic zebrafish heart development. Elucidating how biomechanical Hippo signaling guides heart morphogenesis has direct implications for our understanding of cardiac physiology and pathophysiology.},
  language  = {en}
}
@misc{SeyfriedRoedel2020,
  author    = {Seyfried, Salim and R{\"o}del, Claudia Jasmin},
  title     = {Blood flow matters in a zebrafish model of cerebral cavernous malformations},
  series = {Circulation research : an official journal of the American Heart Association},
  volume    = {126},
  journal   = {Circulation research : an official journal of the American Heart Association},
  number    = {1},
  publisher = {Lippincott Williams \& Wilkins},
  address   = {Baltimore, Md.},
  issn      = {0009-7330},
  doi       = {10.1161/CIRCRESAHA.119.316286},
  pages     = {E1 -- E2},
  year      = {2020},
  language  = {en}
}