@phdthesis{Ghandour2020,
  author    = {Ghandour, Rabea},
  title     = {Identification of chloroplast translational feedback regulation and establishment of aptamer based mRNA purification to unravel involved regulatory factors},
  doi       = {10.25932/publishup-48289},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-482896},
  school      = {Universit{\"a}t Potsdam},
  pages     = {XIII, 173},
  year      = {2020},
  abstract  = {After endosymbiosis, chloroplasts lost most of their genome. Many former endosymbiotic genes are now nucleus-encoded and the products are re-imported post-translationally. Consequently, photosynthetic complexes are built of nucleus- and plastid-encoded subunits in a well-defined stoichiometry. In Chlamydomonas, the translation of chloroplast-encoded photosynthetic core subunits is feedback-regulated by the assembly state of the complexes they reside in. This process is called Control by Epistasy of Synthesis (CES) and enables the efficient production of photosynthetic core subunits in stoichiometric amounts. In chloroplasts of embryophytes, only Rubisco subunits have been shown to be feedback-regulated. That opens the question if there is additional CES regulation in embryophytes. I analyzed chloroplast gene expression in tobacco and Arabidopsis mutants with assembly defects for each photosynthetic complex to broadly answer this question. My results (i) confirmed CES within Rubisco and hint to potential translational feedback regulation in the synthesis of (ii) cytochrome b6f (Cyt b6f) and (iii) photosystem II (PSII) subunits. This work suggests a CES network in PSII that links psbD, psbA, psbB, psbE, and potentially psbH expression by a feedback mechanism that at least partially differs from that described in Chlamydomonas. Intriguingly, in the Cyt b6f complex, a positive feedback regulation that coordinates the synthesis of PetA and PetB was observed, which was not previously reported in Chlamydomonas. No evidence for CES interactions was found in the expression of NDH and ATP synthase subunits of embryophytes. Altogether, this work provides solid evidence for novel assembly-dependent feedback regulation mechanisms controlling the expression of photosynthetic genes in chloroplasts of embryophytes. In order to obtain a comprehensive inventory of the rbcL and psbA RNA-binding proteomes (including factors that regulate their expression, especially factors involved in CES), an aptamer based affinity purification method was adapted and refined for the specific purification these transcripts from tobacco chloroplasts. To this end, three different aptamers (MS2, Sephadex ,and streptavidin binding) were stably introduced into the 3' UTRs of psbA and rbcL by chloroplast transformation. RNA aptamer based purification and subsequent chip analysis (RAP Chip) demonstrated a strong enrichment of psbA and rbcL transcripts and currently, ongoing mass spectrometry analyses shall reveal potential regulatory factors. Furthermore, the suborganellar localization of MS2 tagged psbA and rbcL transcripts was analyzed by a combined affinity, immunology, and electron microscopy approach and demonstrated the potential of aptamer tags for the examination of the spatial distribution of chloroplast transcripts.},
  language  = {en}
}
@phdthesis{Bombart2020,
  author    = {Bombart, Diane},
  title     = {The geometry of a complex institution},
  doi       = {10.25932/publishup-48872},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-488724},
  school      = {Universit{\"a}t Potsdam},
  pages     = {181},
  year      = {2020},
  abstract  = {Organizations incorporate the institutional demands from their environment in order to be deemed legitimate and survive. Yet, complexifying societies promulgate multiple and sometimes inconsistent institutional prescriptions. When these prescriptions collide, organizations are said to face "institutional complexity". How does an organization then incorporate incompatible demands? What are the consequences of institutional complexity for an organization? The literature provides contradictory conceptual and empirical insights on the matter. A central assumption, however, remains that internal incompatibilities generate tensions that, under certain conditions, can escalate into intractable conflicts, resulting in dysfunctionality and loss of legitimacy. The present research is an inquiry into what happens inside an organization when it incorporates complex institutional demands. To answer this question, I focus on how individuals inside an organization interpret a complex institutional prescription. I examine how members of the French Development Agency interpret 'results-based management', a central but complex concept of organizing in the field of development aid. I use an inductive mixed methods design to systematically explore how different interpretations of results-based management relate to one another and to the organizational context in which they are embedded. The results reveal that results-based management is a contested concept in the French Development Agency. I find multiple interpretations of the concept, which are attached to partly incompatible rationales about "who we are" and "what we do as an organization". These rationales nevertheless coexist as balanced forces, without escalating into open conflict. The analysis points to four reasons for this peaceful coexistence of diverging rationales inside one and the same organization: 1) individuals' capacity to manipulate different interpretations of a complex institutional demand, 2) the nature of interpretations, which makes them more or less prone to conflict, 3) the balanced distribution of rationales across the organizational sub-contexts and 4) the shared rules of interpretation provided by the larger socio-cultural context. This research shows that an organization that incorporates institutional complexity comes to represent different, partly incompatible things to its members without being at war with itself. In doing so, it contributes to our knowledge of institutional complexity and organizational hybridity. It also advances our understanding of internal organizational legitimacy and of the translation of managerial concepts in organizations.},
  language  = {en}
}
@misc{BalaKerrigan2021,
  author    = {Bala, Sruti and Kerrigan, Dylan},
  title     = {Embodied Practices - Looking from Small Places},
  series = {Minor Constellations in Conversation Lecture Series},
  journal   = {Minor Constellations in Conversation Lecture Series},
  editor    = {Heide, Johanna},
  doi       = {10.25932/publishup-50899},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-508999},
  year      = {2021},
  abstract  = {"Embodied Practices - Looking From Small Places" is an edited transcript of a conversation between theatre and performance scholar Sruti Bala (University of Amsterdam) and sociologist, criminologist and anthropologist Dylan Kerrigan (University of Leicester) that took place as an online event in November 2020. Throughout their talk, Bala and Kerrigan engage with the legacy of Haitian anthropologist Michel-Rolph Trouillot. Specifically, they focus on his approach of looking from small units, such as small villages in Dominica, outwards to larger political structures such as global capitalism, social inequalities and the distribution of power. They also share insights from their own research on embodied practices in the Caribbean, Europe and India and answer questions such as: What can research on and through embodied practices tell us about systems of power and domination that move between the local and the global? How can performance practices which are informed by multiple locations and cultures be read and appreciated adequately? Sharing insights from his research into Guyanese prisons, Kerrigan outlines how he aims to connect everyday experiences and struggles of Caribbean people to trans-historical and transnational processes such as racial capitalism and post/coloniality. Furthermore, he elaborates on how he uses performance practices such as spoken word poetry and data verbalisation to connect with systematically excluded groups. Bala challenges na{\"i}ve notions about the inherent transformative potential of performance in her research on performance and translation. She points to the way in which performance and its reception is always already inscribed in what she calls global or planetary asymmetries. At the conclusion of this conversation, they broach the question: are small places truly as small as they seem?},
  language  = {en}
}
@misc{HeisselBollmannKangasetal.2021,
  author    = {Heißel, Andreas and Bollmann, J and Kangas, Maria and Rapp, Michael Armin and S{\´a}nchez, Alba Cristina and Abdulla, K},
  title     = {Validation of the German version of the work and social adjustment scale in a sample of depressed patients},
  series = {Zweitver{\"o}ffentlichungen der Universit{\"a}t Potsdam : Humanwissenschaftliche Reihe},
  journal   = {Zweitver{\"o}ffentlichungen der Universit{\"a}t Potsdam : Humanwissenschaftliche Reihe},
  publisher = {Universit{\"a}tsverlag Potsdam},
  address   = {Potsdam},
  issn      = {1866-8364},
  doi       = {10.25932/publishup-54781},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-547810},
  pages     = {1 -- 11},
  year      = {2021},
  abstract  = {Background Depression is one of the key factors contributing to difficulties in one's ability to work, and serves as one of the major reasons why employees apply for psychotherapy and receive insurance subsidization of treatments. Hence, an increasing and growing number of studies rely on workability assessment scales as their primary outcome measure. The Work and Social Assessment Scale (WSAS) has been documented as one of the most psychometrically reliable and valid tools especially developed to assess workability and social functioning in patients with mental health problems. Yet, the application of the WSAS in Germany has been limited due to the paucity of a valid questionnaire in the German language. The objective of the present study was to translate the WSAS, as a brief and easy administrable tool into German and test its psychometric properties in a sample of adults with depression. Methods Two hundred seventy-seven patients (M = 48.3 years, SD = 11.1) with mild to moderately severe depression were recruited. A multistep translation from English into the German language was performed and the factorial validity, criterion validity, convergent validity, discriminant validity, internal consistency, and floor and ceiling effects were examined. Results The confirmatory factor analysis results confirmed the one-factor structure of the WSAS. Significant correlations with the WHODAS 2-0 questionnaire, a measure of functionality, demonstrated good convergent validity. Significant correlations with depression and quality of life demonstrated good criterion validity. The WSAS also demonstrated strong internal consistency (α = .89), and the absence of floor and ceiling effects indicated good sensitivity of the instrument. Conclusions The results of the present study demonstrated that the German version of the WSAS has good psychometric properties comparable to other international versions of this scale. The findings recommend a global assessment of psychosocial functioning with the sum score of the WSAS.},
  language  = {en}
}
@misc{Bunselmeyer2024,
  type      = {Master Thesis},
  author    = {Bunselmeyer, Lena},
  title     = {Die Agenda 2030 in kommunalen Nachhaltigkeitsstrategien},
  doi       = {10.25932/publishup-63487},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-634873},
  school      = {Universit{\"a}t Potsdam},
  pages     = {64},
  year      = {2024},
  abstract  = {Die 2016 verabschiedeten Sustainable Development Goals (SDGs) der Vereinten Nationen sind Referenzrahmen von Nachhaltigkeitsstrategien auf Bundes- Landes- und kommunaler Ebene geworden. St{\"a}dte r{\"u}ckten im Zuge der Agenda 2030 in den Mittelpunkt. Ihre Verwaltungen befinden sich dabei in einem herausfordernden Spannungsfeld: Einerseits haben die SDGs den holistischen Anspruch, vollst{\"a}ndig in das Handeln der Kommunen integriert zu werden. Andererseits ist f{\"u}r eine effektive Umsetzung eine starke Anpassung der SDGs an den lokalen Kontext notwendig. Die vorliegende Arbeit betrachtet anhand einer Fallstudie die Frage, wie Kommunen die Nachhaltigkeitsziele der Vereinten Nationen in ihre Handlungsprogramme und Nachhaltigkeitsstrategien {\"u}bersetzen, und welche Faktoren Einfluss auf diesen Prozess haben. Dabei wird ein translationstheoretischer Ansatz verwendet, der die {\"U}bertragung einer Idee in einen lokalen Kontext als aktiven Transfer versteht, bei dem das Handeln der beteiligten Akteure und deren Konstruktion der aufzunehmenden Idee im Fokus steht. Die Translation wird mit Hilfe von qualitativen Interviews nachvollzogen und analysiert. Die Ergebnisse zeigen, dass die SDGs zwar anhand ihrer Relevanz f{\"u}r die Kommune gefiltert werden, der normative Anspruch der SDGs aber erhalten bleibt und angesichts des als gering beurteilten Fortschritts der Kommune besonderes Gewicht erh{\"a}lt. Zentrale Einflussfaktoren f{\"u}r die Translation sind die verf{\"u}gbaren personellen und finanziellen Ressourcen, die Akzeptanz f{\"u}r die SDGs in Verwaltung, Politik und Gesellschaft und nicht zuletzt das pers{\"o}nliche Engagement einzelner Verwaltungsmitarbeiter*innen.},
  language  = {de}
}