@article{BadalyanDierichStibaetal.2014,
  author    = {Badalyan, Artavazd and Dierich, Marlen and Stiba, Konstanze and Schwuchow, Viola and Leimk{\"u}hler, Silke and Wollenberger, Ulla},
  title     = {Electrical wiring of the aldehyde oxidoreductase PaoABC with a polymer containing osmium redox centers},
  series = {Biosensors},
  volume    = {4},
  journal   = {Biosensors},
  number    = {4},
  publisher = {MDPI},
  address   = {Basel},
  doi       = {10.3390/bios4040403},
  pages     = {403 -- 421},
  year      = {2014},
  abstract  = {Biosensors for the detection of benzaldehyde and g-aminobutyric acid (GABA) are reported using aldehyde oxidoreductase PaoABC from Escherichia coli immobilized in a polymer containing bound low potential osmium redox complexes. The electrically connected enzyme already electrooxidizes benzaldehyde at potentials below -0.15 V (vs. Ag|AgCl, 1 M KCl). The pH-dependence of benzaldehyde oxidation can be strongly influenced by the ionic strength. The effect is similar with the soluble osmium redox complex and therefore indicates a clear electrostatic effect on the bioelectrocatalytic efficiency of PaoABC in the osmium containing redox polymer. At lower ionic strength, the pH-optimum is high and can be switched to low pH-values at high ionic strength. This offers biosensing at high and low pH-values. A "reagentless" biosensor has been formed with enzyme wired onto a screen-printed electrode in a flow cell device. The response time to addition of benzaldehyde is 30 s, and the measuring range is between 10-150 µM and the detection limit of 5 µM (signal to noise ratio 3:1) of benzaldehyde. The relative standard deviation in a series (n = 13) for 200 µM benzaldehyde is 1.9\%. For the biosensor, a response to succinic semialdehyde was also identified. Based on this response and the ability to work at high pH a biosensor for GABA is proposed by coimmobilizing GABA-aminotransferase (GABA-T) and PaoABC in the osmium containing redox polymer.},
  language  = {en}
}
@misc{BadalyanDierichStibaetal.2014,
  author    = {Badalyan, Artavazd and Dierich, Marlen and Stiba, Konstanze and Schwuchow, Viola and Leimk{\"u}hler, Silke and Wollenberger, Ulla},
  title     = {Electrical wiring of the aldehyde oxidoreductase PaoABC with a polymer containing osmium redox centers},
  series = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe},
  journal   = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe},
  number    = {1082},
  issn      = {1866-8372},
  doi       = {10.25932/publishup-47507},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-475070},
  pages     = {21},
  year      = {2014},
  abstract  = {Biosensors for the detection of benzaldehyde and g-aminobutyric acid (GABA) are reported using aldehyde oxidoreductase PaoABC from Escherichia coli immobilized in a polymer containing bound low potential osmium redox complexes. The electrically connected enzyme already electrooxidizes benzaldehyde at potentials below -0.15 V (vs. Ag|AgCl, 1 M KCl). The pH-dependence of benzaldehyde oxidation can be strongly influenced by the ionic strength. The effect is similar with the soluble osmium redox complex and therefore indicates a clear electrostatic effect on the bioelectrocatalytic efficiency of PaoABC in the osmium containing redox polymer. At lower ionic strength, the pH-optimum is high and can be switched to low pH-values at high ionic strength. This offers biosensing at high and low pH-values. A "reagentless" biosensor has been formed with enzyme wired onto a screen-printed electrode in a flow cell device. The response time to addition of benzaldehyde is 30 s, and the measuring range is between 10-150 µM and the detection limit of 5 µM (signal to noise ratio 3:1) of benzaldehyde. The relative standard deviation in a series (n = 13) for 200 µM benzaldehyde is 1.9\%. For the biosensor, a response to succinic semialdehyde was also identified. Based on this response and the ability to work at high pH a biosensor for GABA is proposed by coimmobilizing GABA-aminotransferase (GABA-T) and PaoABC in the osmium containing redox polymer.},
  language  = {en}
}
@phdthesis{Memczak2014,
  author    = {Memczak, Henry},
  title     = {Entwicklung influenzabindender Peptide f{\"u}r die Biosensorik},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-72470},
  school      = {Universit{\"a}t Potsdam},
  pages     = {X, 117},
  year      = {2014},
  abstract  = {Das Influenzavirus infiziert S{\"a}ugetiere und V{\"o}gel. Der erste Schritt im Infektionszyklus ist die Anbindung des Viruses {\"u}ber sein Oberfl{\"a}chenprotein H{\"a}magglutinin (HA) an Zuckerstrukturen auf Epithelzellen des respiratorischen Traktes im Wirtsorganismus. Aus den drei komplementarit{\"a}tsbestimmenden Regionen (complementarity determining regions, CDRs) der schweren Kette eines monoklonalen H{\"a}magglutinin-bindenden Antik{\"o}rpers wurden drei lineare Peptide abgeleitet. Die Bindungseigenschaften der drei Peptide wurden experimentell mittels Oberfl{\"a}chenplasmonenresonanzspektroskopie untersucht. Es zeigte sich, dass in {\"U}bereinstimmung mit begleitenden Molekulardynamik-Simulationen zwei der drei Peptide (PeB und PeC) analog zur Bindef{\"a}higkeit des Antik{\"o}rpers in der Lage sind, Influenzaviren vom Stamm X31 (H3N2 A/Aichi/2/1968) zu binden. Die Interaktion des Peptids PeB, welches potentiell mit der konservierten Rezeptorbindestelle im HA interagiert, wurde anschließend n{\"a}her charakterisiert. Die Detektion der Influenzaviren war unter geeigneten Immobilisationsbedingungen im diagnostisch relevanten Bereich m{\"o}glich. Die Spezifit{\"a}t der PeB-Virus-Bindung wurde mittels geeigneter Kontrollen auf der Seite des Analyten und des Liganden nachgewiesen. Des Weiteren war das Peptid PeB in der Lage die Bindung von X31-Viren an Mimetika seines nat{\"u}rlichen Rezeptors zu inhibieren, was die spezifische Interaktion mit der Rezeptorbindungsstelle im H{\"a}magglutinin belegt. Anschließend wurde die Prim{\"a}rsequenz von PeB durch eine vollst{\"a}ndige Substitutionsanalyse im Microarray-Format hinsichtlich der Struktur-Aktivit{\"a}ts-Beziehungen charakterisiert. Dies f{\"u}hrte außerdem zu verbesserten Peptidvarianten mit erh{\"o}hter Affinit{\"a}t und breiterer Spezifit{\"a}t gegen aktuelle Influenzast{\"a}mme verschiedener Serotypen (z.B. H1N1/2009, H5N1/2004, H7N1/2013). Schließlich konnte durch Verwendung einer in der Prim{\"a}rsequenz angepassten h{\"o}her affinen Peptidvariante die Influenzainfektion in vitro inhibiert werden. Damit stellen die vom urspr{\"u}nglichen Peptid PeB abgeleiteten Varianten Rezeptormolek{\"u}le in biosensorischen Testsystemen sowie potentielle Wirkstoffe dar.},
  language  = {de}
}
@article{HovestaedtMemczakPleineretal.2014,
  author    = {Hovestaedt, Marc and Memczak, Henry and Pleiner, Dennis and Zhang, Xin and Rappich, Joerg and Bier, Frank Fabian and St{\"o}cklein, Walter F. M.},
  title     = {Characterization of a new maleimido functionalization of gold for surface plasmon resonance spectroscopy},
  series = {Journal of molecular recognition : an international journal devoted to research on specific molecular recognition in chemistry, biology, biotechnology and medicine},
  volume    = {27},
  journal   = {Journal of molecular recognition : an international journal devoted to research on specific molecular recognition in chemistry, biology, biotechnology and medicine},
  number    = {12},
  publisher = {Wiley-Blackwell},
  address   = {Hoboken},
  issn      = {0952-3499},
  doi       = {10.1002/jmr.2396},
  pages     = {707 -- 713},
  year      = {2014},
  abstract  = {Para-maleimidophenyl (p-MP) modified gold surfaces have been prepared by one-step electrochemical deposition and used in surface plasmon resonance (SPR) studies. Therefore, a FITC mimotope peptide (MP1, 12 aa), a human mucin 1 epitope peptide (MUC, 9 aa) and a protein with their specific antibodies were used as model systems. The peptides were modified with an N-terminal cysteine for covalent and directed coupling to the maleimido functionalized surface by means of Michael addition. The coupling yield of the peptide, the binding characteristics of antibody and the unspecific adsorption of the analytes were investigated. The results expand the spectrum of biosensors usable with p-MP by widely used SPR and support its potential to be versatile for several electrochemical and optical biosensors. This allows the combination of an electrochemical and optical read-out for a broad variety of biomolecular interactions on the same chip. Copyright (c) 2014 John Wiley \& Sons, Ltd.},
  language  = {en}
}
@article{CywinskiHammannHuehnetal.2014,
  author    = {Cywinski, Piotr J. and Hammann, Tommy and Huehn, Dominik and Parak, Wolfgang J. and Hildebrandt, Niko and L{\"o}hmannsr{\"o}ben, Hans-Gerd},
  title     = {Europium-quantum dot nanobioconjugates as luminescent probes for time-gated biosensing},
  series = {Journal of biomedical optics},
  volume    = {19},
  journal   = {Journal of biomedical optics},
  number    = {10},
  publisher = {SPIE},
  address   = {Bellingham},
  issn      = {1083-3668},
  doi       = {10.1117/1.JBO.19.10.101506},
  pages     = {8},
  year      = {2014},
  abstract  = {Nanobioconjugates have been synthesized using cadmium selenide quantum dots (QDs), europium complexes (EuCs), and biotin. In those conjugates, long-lived photoluminescence (PL) is provided by the europium complexes, which efficiently transfer energy via Forster resonance energy transfer (FRET) to the QDs in close spatial proximity. As a result, the conjugates have a PL emission spectrum characteristic for QDs combined with the long PL decay time characteristic for EuCs. The nanobioconjugates synthesis strategy and photo-physical properties are described as well as their performance in a time-resolved streptavidin-biotin PL assay. In order to prepare the QD-EuC-biotin conjugates, first an amphiphilic polymer has been functionalized with the EuC and biotin. Then, the polymer has been brought onto the surface of the QDs (either QD655 or QD705) to provide functionality and to make the QDs water dispersible. Due to a short distance between EuC and QD, an efficient FRET can be observed. Additionally, the QD-EuC-biotin conjugates' functionality has been demonstrated in a PL assay yielding good signal discrimination, both from autofluorescence and directly excited QDs. These newly designed QD-EuC-biotin conjugates expand the class of highly sensitive tools for bioanalytical optical detection methods for diagnostic and imaging applications. (C) 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)},
  language  = {en}
}