@phdthesis{BartschJodexnis2004, author = {Bartsch, Bernhard and Jodexnis, Marion}, title = {Sexuelle Einstellungen und Verhaltensweisen von StudentenInnen und Krankenpflegesch{\"u}lernInnen unter der Bedrohung durch AIDS : eine empirische Untersuchung an LehramtsstudentenInnen der Primarstufe, DesignstudentenInnen und Krankenpflegesch{\"u}lernInn}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-0001603}, school = {Universit{\"a}t Potsdam}, year = {2004}, abstract = {Die Autoren untersuchten mit Hilfe einer Fragebogenstudie das Sexualverhalten von StudentenInnen und Krankenpflegesch{\"u}lernInnen unter der Bedrohung durch AIDS(n = 593). Als Ergebnis l{\"a}sst sich festhalten, dass unterschiedliche Personengruppen mit unterschiedlichen Einstellungen, mit unterschiedlichem Wissen {\"u}ber HIV und AIDS, mit unterschiedlichem Sexualverhalten sowie einem unterschiedlichen Grad von pers{\"o}nlicher Betroffenheit auf differenzierte Weise angesprochen und zur Pr{\"a}vention angeleitet werden m{\"u}ssen. Die berufliche N{\"a}he zu HIV und AIDS hat keinen Einfluss auf die sexuellen Einstellungen und Verhaltensweisen. Nur durch eine Selbststeuerung kann einer Gefahrensituation, wie sie eine m{\"o}gliche HIV-Infektion darstellt, begegnet werden. Von daher muss neben der pers{\"o}nlichen Betroffenheit auch die Einsicht gegeben sein, dass ich mich als Individuum eigenst{\"a}ndig vor dieser Gefahr sch{\"u}tzen kann. Ferner muss dieses Verhalten in die eigene Lebenswelt eingepasst und von der eigenen sozialen Umgebung getragen werden. Pr{\"a}ventionsbem{\"u}hungen m{\"u}ssen auf kompetenzsteigernde, ressourcenorientierte und differenzierte Maßnahmen setzen. Ans{\"a}tze von Furchtappellen und Lustfeindlichkeit wirken kontraproduktiv. Eine Beschr{\"a}nkung der Pr{\"a}vention auf individuumzentrierte Maßnahmen ist wenig effektiv, sofern gesellschaftliche und strukturelle Bedingungen ausgeblendet werden. Ziel von Sexualp{\"a}dagogik und AIDS-Pr{\"a}ventionsarbeit muss es daher sein, eine von allen geteilte Kommunikationsstruktur f{\"u}r Intimit{\"a}t zu entwickeln.}, language = {de} } @article{FerirVermeireHuskensetal.2011, author = {Ferir, Geoffrey and Vermeire, Kurt and Huskens, Dana and Balzarini, Jan and Van Damme, Els J. M. and Kehr, Jan-Christoph and Dittmann-Th{\"u}nemann, Elke and Swanson, Michael D. and Markovitz, David M. and Schols, Dominique}, title = {Synergistic in vitro anti-HIV type 1 activity of tenofovir with carbohydrate-binding agents (CBAs)}, series = {Antiviral research}, volume = {90}, journal = {Antiviral research}, number = {3}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0166-3542}, doi = {10.1016/j.antiviral.2011.03.188}, pages = {200 -- 204}, year = {2011}, abstract = {Tenofovir, a well-known and highly prescribed anti-HIV-1 drug for the treatment of HIV/AIDS infections, has recently also shown its effectiveness as a potential microbicide drug in the prevention of HIV transmission. Here, we evaluated the combination of tenofovir with various members of the class of carbohydrate-binding agents (CBAs) targeting the glycans on the viral envelope gp120 for their anti-HIV efficacy. The tenofovir/CBA combinations predominantly showed synergistic antiviral activity using the median effect principle. These findings illustrate that combination of tenofovir with CBAs may increase the antiviral potency of the individual drugs and reducing the risk on potential side-effects.}, language = {en} } @phdthesis{Gopalakrishnan2016, author = {Gopalakrishnan, Sathej}, title = {Mathematical modelling of host-disease-drug interactions in HIV disease}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-100100}, school = {Universit{\"a}t Potsdam}, pages = {121}, year = {2016}, abstract = {The human immunodeficiency virus (HIV) has resisted nearly three decades of efforts targeting a cure. Sustained suppression of the virus has remained a challenge, mainly due to the remarkable evolutionary adaptation that the virus exhibits by the accumulation of drug-resistant mutations in its genome. Current therapeutic strategies aim at achieving and maintaining a low viral burden and typically involve multiple drugs. The choice of optimal combinations of these drugs is crucial, particularly in the background of treatment failure having occurred previously with certain other drugs. An understanding of the dynamics of viral mutant genotypes aids in the assessment of treatment failure with a certain drug combination, and exploring potential salvage treatment regimens. Mathematical models of viral dynamics have proved invaluable in understanding the viral life cycle and the impact of antiretroviral drugs. However, such models typically use simplified and coarse-grained mutation schemes, that curbs the extent of their application to drug-specific clinical mutation data, in order to assess potential next-line therapies. Statistical models of mutation accumulation have served well in dissecting mechanisms of resistance evolution by reconstructing mutation pathways under different drug-environments. While these models perform well in predicting treatment outcomes by statistical learning, they do not incorporate drug effect mechanistically. Additionally, due to an inherent lack of temporal features in such models, they are less informative on aspects such as predicting mutational abundance at treatment failure. This limits their application in analyzing the pharmacology of antiretroviral drugs, in particular, time-dependent characteristics of HIV therapy such as pharmacokinetics and pharmacodynamics, and also in understanding the impact of drug efficacy on mutation dynamics. In this thesis, we develop an integrated model of in vivo viral dynamics incorporating drug-specific mutation schemes learned from clinical data. Our combined modelling approach enables us to study the dynamics of different mutant genotypes and assess mutational abundance at virological failure. As an application of our model, we estimate in vivo fitness characteristics of viral mutants under different drug environments. Our approach also extends naturally to multiple-drug therapies. Further, we demonstrate the versatility of our model by showing how it can be modified to incorporate recently elucidated mechanisms of drug action including molecules that target host factors. Additionally, we address another important aspect in the clinical management of HIV disease, namely drug pharmacokinetics. It is clear that time-dependent changes in in vivo drug concentration could have an impact on the antiviral effect, and also influence decisions on dosing intervals. We present a framework that provides an integrated understanding of key characteristics of multiple-dosing regimens including drug accumulation ratios and half-lifes, and then explore the impact of drug pharmacokinetics on viral suppression. Finally, parameter identifiability in such nonlinear models of viral dynamics is always a concern, and we investigate techniques that alleviate this issue in our setting.}, language = {en} } @misc{HeisselZechRappetal.2019, author = {Heissel, Andreas and Zech, Philipp and Rapp, Michael A. and Schuch, Felipe B. and Lawrence, Jimmy B. and Kangas, Maria and Heinzel, Stephan}, title = {Effects of exercise on depression and anxiety in persons living with HIV: A meta-analysis}, series = {Journal of psychosomatic research}, volume = {126}, journal = {Journal of psychosomatic research}, publisher = {Elsevier}, address = {Oxford}, issn = {0022-3999}, doi = {10.1016/j.jpsychores.2019.109823}, pages = {12}, year = {2019}, abstract = {Objective: The purpose of this systematic review and meta-analysis was to examine the effects of exercise on depression and anxiety in people living with HIV (PLWH), and to evaluate, through subgroup analysis, the effects of exercise type, frequency, supervision by exercise professionals, study quality, and control group conditions on these outcomes. Method: A literature search was conducted through four electronic databases from inception to February 2019. Considered for inclusion were randomized controlled trials (RCTs) investigating exercise interventions and depression or anxiety as outcomes in people living with HIV (>= 18 years of age). Ten studies were included (n = 479 participants, 49.67\% females at baseline), and the standardized mean difference (SMD) and heterogeneity were calculated using random-effect models. An additional pre-post meta-analysis was also conducted. Results: A large effect in favor of exercise when compared to controls was found for depression (SMD = -0.84, 95\%CI = [-1.57, -0.11], p = 0.02) and anxiety (SMD = -1.23, 95\%CI = [-2.42, 0.04], p = -0.04). Subgroup analyses for depression revealed large effects on depression for aerobic exercise only (SMD = -0.96, 95\%CI = [-1.63, -0.30], p = 0.004), a frequency of >= 3 exercise sessions per week (SMD = -1.39, 95\%CI = [-2.24, -0.54], p < 0.001), professionally supervised exercise (SMD = -1.40, 95\%CI = [-2.46, -0.17], p = 0.03]), and high-quality studies (SMD = -1.31, 95\%CI = [-2.46, -0.17], p = 0.02). Conclusion: Exercise seems to decrease depressive symptoms and anxiety in PLWH, but other larger and high-quality studies are needed to verify these effects.}, language = {en} } @phdthesis{Hoelscher2020, author = {Hoelscher, Matthijs Pieter}, title = {The production of antimicrobial polypeptides in chloroplasts}, school = {Universit{\"a}t Potsdam}, pages = {xiii, 114}, year = {2020}, abstract = {Plants are an attractive platform for the production of medicinal compounds because of their potential to generate large amounts of biomass cheaply. The use of chloroplast transformation is an attractive way to achieve the recombinant production of proteins in plants, because of the chloroplasts' high capacity to produce foreign proteins in comparison to nuclear transformed plants. In this thesis, the production of two different types of antimicrobial polypeptides in chloroplasts is explored. The first example is the production of the potent HIV entry inhibitor griffithsin. Griffithsin has the potential to prevent HIV infections by blocking the entry of the virus into human cells. Here the use of transplastomic plants as an inexpensive production method for griffithsin was explored. Transplastomic plants grew healthily and were able to accumulate griffithsin to up to 5\% of the total soluble protein. Griffithsin could easily be purified from tobacco leaf tissue and had a similarly high neutralization activity as griffithsin recombinantly produced in bacteria. Griffithsin could be purified from dried tobacco leaves, demonstrating that dried leaves could be used as a storable starting material for griffithsin purification, circumventing the need for immediate purification after harvest. The second example is the production of antimicrobial peptides (AMPs) that have the capacity to kill bacteria and are an attractive alternative to currently used antibiotics that are increasingly becoming ineffective. The production of antimicrobial peptides was considerably more challenging than the production of griffithsin. Small AMPs are prone to degradation in plastids. This problem was overcome by fusing AMPs to generate larger polypeptides. In one approach, AMPs were fused to each other to increase size and combine the mode of action of multiple AMPs. This improved the accumulation of AMPs but also resulted in impaired plant growth. This was solved by the use of two different inducible systems, which could largely restore plant growth. Fusions of multiple AMPs were insoluble and could not be purified. In addition to fusing AMPs to each other, the fusion of AMPs to small ubiquitin-like modifier (SUMO), was tested as an approach to improve the accumulation, facilitate purification, and reduce the toxicity of AMPs to chloroplasts. Fusion of AMPs to SUMO indeed increased accumulation while reducing the toxicity to the plants. SUMO fusions produced inside chloroplasts could be purified, and SUMO could be efficiently cleaved off with the SUMO protease. Such fusions therefore provide a promising strategy for the production of AMPs and other small polypeptides inside chloroplasts.}, language = {en} } @misc{IdzikCywinskiCranfieldetal.2011, author = {Idzik, Krzysztof Ryszard and Cywinski, Piotr J. and Cranfield, Charles G. and Mohr, Gerhard J. and Beckert, Rainer}, title = {Molecular recognition of the antiretroviral drug Abacavir}, series = {Postprints der Universit{\"a}t Potsdam : Mathematisch Naturwissenschaftliche Reihe}, journal = {Postprints der Universit{\"a}t Potsdam : Mathematisch Naturwissenschaftliche Reihe}, number = {847}, issn = {1866-8372}, doi = {10.25932/publishup-43037}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-430372}, pages = {1195 -- 1204}, year = {2011}, abstract = {Due to their optical and electro-conductive attributes, carbazole derivatives are interesting materials for a large range of biosensor applications. In this study, we present the synthesis routes and fluorescence evaluation of newly designed carbazole fluorosensors that, by modification with uracil, have a special affinity for antiretroviral drugs via either Watson-Crick or Hoogsteen base pairing. To an N-octylcarbazole-uracil compound, four different groups were attached, namely thiophene, furane, ethylenedioxythiophene, and another uracil; yielding four different derivatives. Photophysical properties of these newly obtained derivatives are described, as are their interactions with the reverse transcriptase inhibitors such as abacavir, zidovudine, lamivudine and didanosine. The influence of each analyte on biosensor fluorescence was assessed on the basis of the Stern-Volmer equation and represented by Stern-Volmer constants. Consequently we have demonstrated that these structures based on carbazole, with a uracil group, may be successfully incorporated into alternative carbazole derivatives to form biosensors for the molecular recognition of antiretroviral drugs.}, language = {en} } @article{IdzikCywinskiCranfieldetal.2011, author = {Idzik, Krzysztof Ryszard and Cywinski, Piotr J. and Cranfield, Charles G. and Mohr, Gerhard J. and Beckert, Rainer}, title = {Molecular recognition of the antiretroviral drug abacavir towards the development of a novel carbazole-based fluorosensor}, series = {Journal of fluorescence}, volume = {21}, journal = {Journal of fluorescence}, number = {3}, publisher = {Springer}, address = {New York}, issn = {1053-0509}, doi = {10.1007/s10895-010-0798-7}, pages = {1195 -- 1204}, year = {2011}, abstract = {Due to their optical and electro-conductive attributes, carbazole derivatives are interesting materials for a large range of biosensor applications. In this study, we present the synthesis routes and fluorescence evaluation of newly designed carbazole fluorosensors that, by modification with uracil, have a special affinity for antiretroviral drugs via either Watson-Crick or Hoogsteen base pairing. To an N-octylcarbazole-uracil compound, four different groups were attached, namely thiophene, furane, ethylenedioxythiophene, and another uracil; yielding four different derivatives. Photophysical properties of these newly obtained derivatives are described, as are their interactions with the reverse transcriptase inhibitors such as abacavir, zidovudine, lamivudine and didanosine. The influence of each analyte on biosensor fluorescence was assessed on the basis of the Stern-Volmer equation and represented by Stern-Volmer constants. Consequently we have demonstrated that these structures based on carbazole, with a uracil group, may be successfully incorporated into alternative carbazole derivatives to form biosensors for the molecular recognition of antiretroviral drugs.}, language = {en} } @misc{LoeckmannSchneider2022, author = {L{\"o}ckmann, Teresa and Schneider, Matthias}, title = {Geschlechtergerechtigkeit im Verbraucher_innenschutz?}, series = {Genderblog}, journal = {Genderblog}, publisher = {Zentrum f{\"u}r transdisziplin{\"a}re Geschlechterstudien an der Humboldt-Universit{\"a}t zu Berlin}, address = {Berlin}, year = {2022}, language = {de} } @phdthesis{PerezChaparro2022, author = {P{\´e}rez Chaparro, Camilo Germ{\´a}n Alberto}, title = {Non-HIV comorbidities and exercise in German people living with HIV}, doi = {10.25932/publishup-56084}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-560842}, school = {Universit{\"a}t Potsdam}, pages = {149}, year = {2022}, abstract = {The post-antiretroviral therapy era has transformed HIV into a chronic disease and non-HIV comorbidities (i.e., cardiovascular and mental diseases) are more prevalent in PLWH. The source of these non-HIV comorbidities aside from traditional risk factor include HIV infection, inflammation, distorted immune activation, burden of chronic diseases, and unhealthy lifestyle like sedentarism. Exercise is known for its beneficial effects in mental and physical health; reasons why exercise is recommended to prevent and treat difference cardiovascular and mental diseases in the general population. This cumulative thesis aimed to comprehend the relation exercise has to non-HIV comorbidities in German PLWH. Four studies were conducted to 1) understand exercise effects in cardiorespiratory fitness and muscle strength on PLWH through a systematic review and meta-analyses and 2) determine the likelihood of German PLWH developing non-HIV comorbidities, in a cross-sectional study. Meta-analytic examination indicates PLWH cardiorespiratory fitness (VO2max SMD = 0.61 ml·kg·min-1, 95\% CI: 0.35-0.88, z = 4.47, p < 0.001, I2 = 50\%) and strength (of remark lowerbody strength by 16.8 kg, 95\% CI: 13-20.6, p< 0.001) improves after an exercise intervention in comparison to a control group. Cross-sectional data suggest exercise has a positive effect on German PLWH mental health (less anxiety and depressive symptoms) and protects against the development of anxiety (PR: 0.57, 95\%IC: 0.36 - 0.91, p = 0.01) and depression (PR: 0.62, 95\%IC: 0.41 - 0.94, p = 0.01). Likewise, exercise duration is related to a lower likelihood of reporting heart arrhythmias (PR: 0.20, 95\%IC: 0.10 - 0.60, p < 0.01) and exercise frequency to a lower likelihood of reporting diabetes mellitus (PR: 0.40, 95\%IC: 0.10 - 1, p < 0.01) in German PLWH. A preliminary recommendation for German PLWH who want to engage in exercise can be to exercise ≥ 1 time per week, at an intensity of 5 METs per session or > 103 MET·min·day-1, with a duration ≥ 150 minutes per week. Nevertheless, further research is needed to comprehend exercise dose response and protective effect for cardiovascular diseases, anxiety, and depression in German PLWH.}, language = {en} } @article{PerezChaparroKangasZechetal.2022, author = {P{\´e}rez Chaparro, Camilo Germ{\´a}n Alberto and Kangas, Maria and Zech, Philipp and Schuch, Felipe B. and Rapp, Michael A. and Heißel, Andreas}, title = {Recreational exercise is associated with lower prevalence of depression and anxiety and better quality of life in German people living with HIV}, series = {AIDS care : psychological and socio-medical aspects of AIDS/HIV}, volume = {34}, journal = {AIDS care : psychological and socio-medical aspects of AIDS/HIV}, number = {2}, publisher = {Taylor \& Francis Group}, address = {London [u.a.]}, issn = {1360-0451}, doi = {10.1080/09540121.2021.1889951}, pages = {182 -- 187}, year = {2022}, abstract = {Sedentarism is a risk factor for depression and anxiety. People living with the human immunodeficiency virus (PLWH) have a higher prevalence of anxiety and depression compared to HIV-negative individuals. This cross-sectional study (n = 450, median age 44 (19-75), 7.3\% females) evaluates the prevalence rates and prevalence ratio (PR) of anxiety and/or depression in PLWH associated with recreational exercise. A decreased likelihood of having anxiety (PR=0.57; 0.36-0.91; p = 0.01), depression (PR=0.41; 0.36-0.94; p=0.01), and comorbid anxiety and depression (PR = 0,43; 0.24-0.75; p=0.002) was found in exercising compared to non-exercising PLWH. Recreational exercise is associated with a lower risk for anxiety and/or depression. Further prospective studies are needed to provide insights on the direction of this association.}, language = {en} }