@article{BaxaCooperWeintraubetal.2001, author = {Baxa, Ulrich and Cooper, Alan and Weintraub, N. and Pfeil, Wolfgang and Seckler, Robert}, title = {Enthalpic barriers to the hydrophobic binding of oligosaccharides to phage P22 tailspike protein}, year = {2001}, language = {en} } @article{BurovaBeckertUhlmannetal.1996, author = {Burova, Tatjana V. and Beckert, Vita and Uhlmann, Heike and Ristau, Otto and Bernhardt, Rita and Pfeil, Wolfgang}, title = {Conformational stability of adrenodoxin mutant proteins}, year = {1996}, language = {en} } @article{BurovaBernhardtPfeil1995, author = {Burova, Tatjana V. and Bernhardt, Rita and Pfeil, Wolfgang}, title = {Conformational stability of holo and apo adrenodoxin : a scanning calorimetric study}, year = {1995}, language = {en} } @article{DamaschunDamaschunGastetal.1993, author = {Damaschun, Gregor and Damaschun, Hilde and Gast, Klaus and Misselwitz, Rolf and M{\"u}ller, J{\"u}rgen J. and Pfeil, Wolfgang and Zirwer, Dietrich}, title = {Cold denaturation-induced conformational changes in phosphoglycerate kinase from yeast}, year = {1993}, language = {en} } @article{DudichZavylovPfeiletal.1995, author = {Dudich, Igor V. and Zav`ylov, Vladimir and Pfeil, Wolfgang and Gaestel, Matthias and Zav`yalova, Galina A. and Denesyuk, Alexandr I. and Korpela, Timo}, title = {Dimer structure as a minimum cooperative subunit of small heat shock-proteins}, year = {1995}, language = {en} } @article{FreibergMachnerPfeiletal.2004, author = {Freiberg, Alexander and Machner, M. P. and Pfeil, Wolfgang and Schubert, W. D. and Heinz, Dirk W. and Seckler, Robert}, title = {Folding and stability of the leucine-rich repeat domain of internalin B from Listeria monocytogenes}, issn = {0022-2836}, year = {2004}, abstract = {Internalin B (InlB), a surface protein of the human pathogen Listeria monocytogenes, promotes invasion into various host cell types by inducing phagocytosis of the entire bacterium. The N-terminal half of InlB (residues 36-321, InlB(321)), which is sufficient for this process, contains a central leucine-rich repeat (LRR) domain that is flanked by a small a-helical cap 2 and an immunoglobulin (Ig)-like domain. Here we investigated the variant lacking the Ig-like domain (lnlB(248)). The circular dichroism spectra of both protein variants in the far ultraviolet region are very similar, with a characteristic minimum found at similar to200 nm, possibly resulting from the high 3(10)-helical content in the LRR domain. Upon addition of chemical denaturants, both variants unfold in single transitions with unusually high cooperativity that are fully reversible and best described by two-state equilibria. The free energies of GdmCl-induced unfolding determined from transitions at 20degreesC are 9.9(+/- 0.8)kcal/mol for InlB(321) and 5.4(+/- 0.4) kcal/mol for InlB(248). InlB(321) is also more stable against thermal denaturation, as observed by scanning calorimetry. This suggests, that the Ig-like domain, which presumably does not directly interact with the host cell receptor during bacterial invasion, plays a critical role for the in vivo stability of InlB. (C) 2004 Elsevier Ltd. All rights reserved}, language = {en} } @article{GastDamaschunDesmadriletal.1995, author = {Gast, Klaus and Damaschun, Gregor and Desmadril, Michel and Minard, Philippe and M{\"u}ller-Frohne, Marlies and Pfeil, Wolfgang and Zirwer, Dietrich}, title = {Cold denaturation of yeast phosphoglycerate kinase : which domain is more stable?}, year = {1995}, language = {en} } @article{GesierichPfeil1996, author = {Gesierich, Ulrike and Pfeil, Wolfgang}, title = {The conformational stability of - crystallin is rather low : calorimetric results}, year = {1996}, language = {en} } @article{GoderBeckertPfeiletal.1998, author = {Goder, V. and Beckert, Vita and Pfeil, Wolfgang and Bernhardt, Rita}, title = {Impact of the presequence of a mitochondrium targeted precursor, preadrenodoxin, on folding, catalytic activity and stability of the protein in vitro}, year = {1998}, language = {en} } @article{JungPfeilKoepkeetal.1994, author = {Jung, Christiane and Pfeil, Wolfgang and K{\"o}pke, Karla and Schulze, Heike and Ristau, Otto}, title = {Conformational states and substates of cytochrome P450cam - insight in protein dynamics and folding}, year = {1994}, language = {en} }