@book{vanKleunen2000, author = {van Kleunen, Mark}, title = {Zur Messung der Teuerung : Konferenzbeitr{\"a}ge des 5. Erfahrungsaustausches am 22. und 23. Juni 2000 an der Universit{\"a}t Potsdam}, series = {Statistische Diskussionsbeitr{\"a}ge}, volume = {Sonderband 2000}, journal = {Statistische Diskussionsbeitr{\"a}ge}, editor = {Strohe, Hans Gerhard and Elsner, Eckart}, publisher = {Univ.}, address = {Potsdam}, issn = {0949-068x}, pages = {138 S.}, year = {2000}, language = {de} } @article{vanKleunenFischer2003, author = {van Kleunen, Mark and Fischer, Markus}, title = {Effects of four generations of density-dependent selection on life history traits and their plasticity in a clonally propagated plant}, issn = {1010- 061X}, year = {2003}, language = {en} } @article{FischervanKleunenSchmid2004, author = {Fischer, Markus and van Kleunen, Mark and Schmid, Bernhard}, title = {Experimental life-history evolution: selection on the growth form of a clonal plant on its plasticity}, issn = {1010- 061x}, year = {2004}, abstract = {The growth form along the continuum from compact phalanx plants to more loosely packed guerilla plants is an important life-history trait in clonal plants. Prerequisite for its evolution is heritable genetic variation. Starting with 102 genotypes of the stoloniferous herb Ranunculus reptans, we performed one selection experiment on spatial spread per rosette as measure of guerillaness (broad-sense heritability 0.198) and another on plasticity in this trait in response to competition (broad-sense heritability 0.067). After two generations, spatial spread was 36.9\% higher in the high line than in the low line (realized heritability +/- SE 0.149 +/- 0.039). Moreover, compared with the low line genotypes of the high line had fewer rosettes, a lower proportion of flowering rosettes, a higher proportion of rooted rosettes, more branches per rosette, longer internodes and longer leaves. In the second experiment, we found no significant direct response to selection for high and low plasticity in spatial spread (realized heritability +/- SE - 0.029 +/- 0.063), despite a significant correlated response in plasticity in the length of the first three stolon internodes. Our study indicates a high potential for further evolution of the clonal growth form in R. reptans, but not for its plasticity, and it demonstrates that the clonal growth form does not evolve independently of other clonal life- history characteristics}, language = {en} } @article{vanKleunenRitland2004, author = {van Kleunen, Mark and Ritland, K.}, title = {Predicting evolution of floral traits associated with mating system in a natural plant population}, issn = {1010- 061x}, year = {2004}, abstract = {Evolution of floral traits requires that they are heritable, that they affect fitness, and that they are not constrained by genetic correlations. These prerequisites have only rarely been examined in natural populations. For Mimulus guttatus, we found by using the Riska-method that corolla width, anther length, ovary length and number of red dots on the corolla were heritable in a natural population. Seed production (maternal fitness) was directly positively affected by corolla width and anther size, and indirectly so by ovary length and number of red dots on the corolla. The siring success (paternal fitness), as estimated from allozyme data, was directly negatively affected by anther-stigma separation, and indirectly so by the corolla length-width ratio. Genetic correlations, estimated with the Lynch-method, were positive between floral size measures. We predict that larger flowers with larger reproductive organs, which generally favour outcrossing, will evolve in this natural population of M. guttatus}, language = {en} } @article{vanKleunenLenssenFischeretal.2004, author = {van Kleunen, Mark and Lenssen, J. P. M. and Fischer, Markus and de Kroon, H.}, title = {Local adaption of the clonal plant Ranunculus reptans to flooding along a small-scale gradient.}, year = {2004}, language = {en} } @article{vanKleunenRamponiSchmid2004, author = {van Kleunen, Mark and Ramponi, G. and Schmid, Bernhard}, title = {Effects of herbivory simulated by clipping and jasmonic acid on growth and reproduction in Solidago canadensis}, year = {2004}, language = {en} } @article{vanKleunenRitland2005, author = {van Kleunen, Mark and Ritland, K.}, title = {Estimating heritabilities and genetic correlations with marker-based methods : an experimental test in Mimulus guttatus}, year = {2005}, abstract = {The calculation of heritabilities and genetic correlations, which are necessary for predicting evolutionary responses, requires knowledge about the relatedness between individuals. This information is often not directly available, especially not for natural populations, but can be inferred by using molecular markers such as allozymes. Several methods based on inferred relatedness from marker data have been developed to estimate heritabilities and genetic correlations in natural populations. Most methods use maximum-likelihood procedures to assign pairs or groups of individuals to predefined discrete relatedness classes (e.g., half sibs and unrelated individuals). The Ritland method, on the other hand, uses method of moments estimators to estimate pairwise relatedness among individuals as continuous values. We tested both the Ritland method and a maximum-likelihood method by applying them to a greenhouse population consisting of seed families of the herb Mimulus guttatus and comparing the results to the ones from a frequently used standard method based on half-sib families. Estimates of genetic correlations were far from accurate, especially when we used the Ritland method. However, this study shows that even with a few variable allozyme loci, it is possible to get qualitatively good indications about the presence of heritable genetic variation from marker-based methods, even though both methods underestimated it}, language = {en} } @article{vanKleunenJohnson2005, author = {van Kleunen, Mark and Johnson, S. D.}, title = {Testing for ecological and genetic Allee effects in the invasive shrub Senna didymobotrya (Fabaceae)}, year = {2005}, abstract = {For an introduced plant species to become invasive, it must be able to reproduce even in initially small populations We tested for Aliee effects (reduced reproductive performance of individuals in small populations) in the nonclonal, NW-pollinated shrub Senna didymobotrya in its invasive range in South Africa. The species is self-compatible. but we found that in its invasive range in South Africa it requires pollinators to set seed. Nearly all stigmas (90\%) received pollen. but natural fruit set has very low (3-20\%), Pollen receipt and fruit set were not significantly correlated with population size. We thus found no evidence for an ecological Alice effect arising from pollen limitation in small populations. Offspring seedling performance, measured in terms of stern volume and leaf area, was also not significantly correlated with the number of plants in the source population. indicating that genetic Alice effects, such as inbreeding depression, are either absent or of such a small magnitude that they would be unlikely to limit further spread of S. didymobotrya in South Africa}, language = {en} } @article{vanKleunenFischerSchmid2005, author = {van Kleunen, Mark and Fischer, Markus and Schmid, Bernhard}, title = {Three generations under low versus high neighborhood density affect the life history of a clonal plant through differential selection and genetic drift}, year = {2005}, abstract = {We tested whether neighborhood density affects the clonal life history of the stoloniferous plant Ranunculus reptans through selection and genetic drift. After three generations of sexual reproduction of 16 low- and 16 high- density lines, we studied traits related to growth form and reproduction in a common competition free environment. A 7.7\% lower branching frequency and slightly longer internodes indicated an evolutionary shift towards a less compact growth form under high neighborhood density, but because stolons grew also more vertically, horizontal spread per ramet was slightly decreased. Neighborhood density had no directional effects on the evolution of allocation to sexual and vegetative reproduction in R. reptans. Variation among replicated high-density lines was significantly lower than among replicated low-density lines in both growth form and reproductive characteristics, indicating less pronounced genetic drift under high neighborhood density. This study demonstrates that a clonal plant can respond to selection imposed by neighborhood density. Moreover, it shows that the effect of random genetic drift increases with decreasing neighborhood density. In a declining species, such as R. reptans in central Europe, this may lower the potential for adaptive evolutionary change and increase extinction risk}, language = {en} } @article{vanKleunenFischer2005, author = {van Kleunen, Mark and Fischer, Markus}, title = {Constraints on the evolution of adaptive phenotypic plasticity in plants}, year = {2005}, abstract = {The high potential fitness benefit of phenotypic plasticity tempts us to expect phenotypic plasticity as a frequent adaptation to environmental heterogeneity. Examples of proven adaptive plasticity in plants, however, are scarce and most plastic responses actually may be 'passive' rather than adaptive. This suggests that frequently requirements for the evolution of adaptive plasticity are not met or that such evolution is impeded by constraints. Here we outline requirements and potential constraints for the evolution of adaptive phenotypic plasticity, identify open questions, and propose new research approaches. Important open questions concern the genetic background of plasticity, genetic variation in plasticity, selection for plasticity in natural habitats, and the nature and occurrence of costs and limits of plasticity. Especially promising tools to address these questions are selection gradient analysis, meta-analysis of studies on genotype-by-environment interactions, QTL analysis, cDNA-microarray scanning and quantitative PCR to quantify gene expression, and two-dimensional gel electrophoresis to quantify protein expression. Studying plasticity along the pathway from gene expression to the phenotype and its relationship with fitness will help us to better understand why adaptive plasticity is not more universal, and to more realistically predict the evolution of plastic responses to environmental change}, language = {en} }