@article{VallerianiIgnatovaNagaretal.2010,
  author    = {Valleriani, Angelo and Ignatova, Zoya and Nagar, Apoorva and Lipowsky, Reinhard},
  title     = {Turnover of messenger RNA : polysome statistics beyond the steady state},
  issn      = {0295-5075},
  doi       = {10.1209/0295-5075/89/58003},
  year      = {2010},
  abstract  = {The interplay between turnover or degradation and ribosome loading of messenger RNA (mRNA) is studied theoretically using a stochastic model that is motivated by recent experimental results. Random mRNA degradation affects the statistics of polysomes, i.e., the statistics of the number of ribosomes per mRNA as extracted from cells. Since ribosome loading of newly created mRNA chains requires some time to reach steady state, a fraction of the extracted mRNA/ ribosome complexes does not represent steady state conditions. As a consequence, the mean ribosome density obtained from the extracted complexes is found to be inversely proportional to the mRNA length. On the other hand, the ribosome density profile shows an exponential decrease along the mRNA for prokaryotes and becomes uniform in eukaryotic cells. Copyright (C) EPLA, 2010},
  language  = {en}
}
@article{ZhangFedyuninMiekleyetal.2010,
  author    = {Zhang, Gong and Fedyunin, Ivan and Miekley, Oskar and Valleriani, Angelo and Moura, Alessandro and Ignatova, Zoya},
  title     = {Global and local depletion of ternary complex limits translational elongation},
  issn      = {0305-1048},
  doi       = {10.1093/Nar/Gkq196},
  year      = {2010},
  abstract  = {The translation of genetic information according to the sequence of the mRNA template occurs with high accuracy and fidelity. Critical events in each single step of translation are selection of transfer RNA (tRNA), codon reading and tRNA-regeneration for a new cycle. We developed a model that accurately describes the dynamics of single elongation steps, thus providing a systematic insight into the sensitivity of the mRNA translation rate to dynamic environmental conditions. Alterations in the concentration of the aminoacylated tRNA can transiently stall the ribosomes during translation which results, as suggested by the model, in two outcomes: either stress-induced change in the tRNA availability triggers the premature termination of the translation and ribosomal dissociation, or extensive demand for one tRNA species results in a competition between frameshift to an aberrant open-reading frame and ribosomal drop-off. Using the bacterial Escherichia coli system, we experimentally draw parallels between these two possible mechanisms.},
  language  = {en}
}