@article{VaraoMouraAparecidoRosiniSilvaDomingosSantodaSilvaetal.2022,
  author    = {Var{\~a}o Moura, Alexandre and Aparecido Rosini Silva, Alex and Domingos Santo da Silva, Jos{\´e} and Aleixo Leal Pedroza, Lucas and Bornhorst, Julia and Stiboller, Michael and Schwerdtle, Tanja and Gubert, Priscila},
  title     = {Determination of ions in Caenorhabditis elegans by ion chromatography},
  series = {Journal of chromatography. B},
  volume    = {1204},
  journal   = {Journal of chromatography. B},
  publisher = {Elsevier},
  address   = {Amsterdam [u.a.]},
  issn      = {1570-0232},
  doi       = {10.1016/j.jchromb.2022.123312},
  pages     = {6},
  year      = {2022},
  abstract  = {The Caenorhabditis elegans (C. elegans) is a model organism that has been increasingly used in health and environmental toxicity assessments. The quantification of such elements in vivo can assist in studies that seek to relate the exposure concentration to possible biological effects. Therefore, this study is the first to propose a method of quantitative analysis of 21 ions by ion chromatography (IC), which can be applied in different toxicity studies in C. elegans. The developed method was validated for 12 anionic species (fluoride, acetate, chloride, nitrite, bromide, nitrate, sulfate, oxalate, molybdate, dichromate, phosphate, and perchlorate), and 9 cationic species (lithium, sodium, ammonium, thallium, potassium, magnesium, manganese, calcium, and barium). The method did not present the presence of interfering species, with R2 varying between 0.9991 and 0.9999, with a linear range from 1 to 100 mu g L-1. Limits of detection (LOD) and limits of quantification (LOQ) values ranged from 0.2319 mu g L-1 to 1.7160 mu g L-1 and 0.7028 mu g L-1 to 5.1999 mu g L-1, respectively. The intraday and interday precision tests showed an Relative Standard Deviation (RSD) below 10.0 \% and recovery ranging from 71.0 \% to 118.0 \% with a maximum RSD of 5.5 \%. The method was applied to real samples of C. elegans treated with 200 uM of thallium acetate solution, determining the uptake and bioaccumulated Tl+ content during acute exposure.},
  language  = {en}
}
@article{NicolaiWittFrieseetal.2022,
  author    = {Nicolai, Merle Marie and Witt, Barbara and Friese, Sharleen and Michaelis, Vivien and H{\"o}lz-Armstrong, Lisa and Martin, Maximilian and Ebert, Franziska and Schwerdtle, Tanja and Bornhorst, Julia},
  title     = {Mechanistic studies on the adverse effects of manganese overexposure in differentiated LUHMES cells},
  series = {Food and chemical toxicology},
  volume    = {161},
  journal   = {Food and chemical toxicology},
  publisher = {Elsevier},
  address   = {Oxford},
  issn      = {0278-6915},
  doi       = {10.1016/j.fct.2022.112822},
  pages     = {10},
  year      = {2022},
  abstract  = {Manganese (Mn) is an essential trace element, but overexposure is associated with toxicity and neurological dysfunction. Accumulation of Mn can be observed in dopamine-rich regions of the brain in vivo and Mn-induced oxidative stress has been discussed extensively. Nevertheless, Mn-induced DNA damage, adverse effects of DNA repair, and possible resulting consequences for the neurite network are not yet characterized. For this, LUHMES cells were used, as they differentiate into dopaminergic-like neurons and form extensive neurite networks. Experiments were conducted to analyze Mn bioavailability and cytotoxicity of MnCl2, indicating a dose-dependent uptake and substantial cytotoxic effects. DNA damage, analyzed by means of 8-oxo-7,8-dihydro-2'-guanine (8oxodG) and single DNA strand break formation, showed significant dose- and time-dependent increase of DNA damage upon 48 h Mn exposure. Furthermore, the DNA damage response was increased which was assessed by analytical quantification of poly(ADP-ribosyl)ation (PARylation). Gene expression of the respective DNA repair genes was not significantly affected. Degradation of the neuronal network is significantly altered by 48 h Mn exposure. Altogether, this study contributes to the characterization of Mn-induced neurotoxicity, by analyzing the adverse effects of Mn on genome integrity in dopaminergic-like neurons and respective outcomes.},
  language  = {en}
}
@article{IjomoneIroegbuMorcilloetal.2022,
  author    = {Ijomone, Omamuyovwi M. and Iroegbu, Joy D. and Morcillo, Patricia and Ayodele, Akinyemi J. and Ijomone, Olayemi K. and Bornhorst, Julia and Schwerdtle, Tanja and Aschner, Michael},
  title     = {Sex-dependent metal accumulation and immunoexpression of Hsp70 and Nrf2 in rats' brain following manganese exposure},
  series = {Environmental toxicology},
  volume    = {37},
  journal   = {Environmental toxicology},
  number    = {9},
  publisher = {Wiley},
  address   = {New York, NY},
  issn      = {1520-4081},
  doi       = {10.1002/tox.23583},
  pages     = {2167 -- 2177},
  year      = {2022},
  abstract  = {Manganese (Mn), although important for multiple cellular processes, has posed environmental health concerns due to its neurotoxic effects. In recent years, there have been extensive studies on the mechanism of Mn-induced neuropathology, as well as the sex-dependent vulnerability to its neurotoxic effects. Nonetheless, cellular mechanisms influenced by sex differences in susceptibility to Mn have yet to be adequately characterized. Since oxidative stress is a key mechanism of Mn neurotoxicity, here, we have probed Hsp70 and Nrf2 proteins to investigate the sex-dependent changes following exposure to Mn. Male and female rats were administered intraperitoneal injections of MnCl2 (10 mg/kg and 25 mg/kg) 48 hourly for a total of eight injections (15 days). We evaluated changes in body weight, as well as Mn accumulation, Nrf2 and Hsp70 expression across four brain regions; striatum, cortex, hippocampus and cerebellum in both sexes. Our results showed sex-specific changes in body-weight, specifically in males but not in females. Additionally, we noted sex-dependent accumulation of Mn in the brain, as well as in expression levels of Nrf2 and Hsp70 proteins. These findings revealed sex-dependent susceptibility to Mn-induced neurotoxicity corresponding to differential Mn accumulation, and expression of Hsp70 and Nrf2 across several brain regions.},
  language  = {en}
}
@inproceedings{MichaelisAengenheisterSchwerdtleetal.2021,
  author    = {Michaelis, Vivien and Aengenheister, Leonie and Schwerdtle, Tanja and Buerki-Thurnherr, Tina and Bornhorst, Julia},
  title     = {Manganese translocation across an in vitro model of human villous trophoblast},
  series = {Placenta},
  volume    = {112},
  booktitle = {Placenta},
  publisher = {Elsevier},
  address   = {Amsterdam [u.a.]},
  issn      = {0143-4004},
  pages     = {E63 -- E64},
  year      = {2021},
  language  = {en}
}
@article{KuhnTavaresJacquesTeixeiraetal.2021,
  author    = {Kuhn, Eug{\^e}nia Carla and Tavares Jacques, Maur{\´i}cio and Teixeira, Daniela and Meyer, S{\"o}ren and Gralha, Thiago and Roehrs, Rafael and Camargo, Sandro and Schwerdtle, Tanja and Bornhorst, Julia and {\´A}vila, Daiana Silva},
  title     = {Ecotoxicological assessment of Uruguay River and affluents pre- and biomonitoring},
  series = {Environmental science and pollution research : ESPR},
  volume    = {28},
  journal   = {Environmental science and pollution research : ESPR},
  number    = {17},
  publisher = {Springer},
  address   = {Berlin ; Heidelberg},
  issn      = {0944-1344},
  doi       = {10.1007/s11356-020-11986-4},
  pages     = {21730 -- 21741},
  year      = {2021},
  abstract  = {Uruguay River is the most important river in western Rio Grande do Sul, separating Brazil from Argentina and Uruguay. However, its pollution is of great concern due to agricultural activities in the region and the extensive use of pesticides. In a long term, this practice leads to environmental pollution, especially to the aquatic system. The objective of this study was to analyze the physicochemical characteristics, metals and pesticides levels in water samples obtained before and after the planting and pesticides' application season from three sites: Uruguay River and two minor affluents, Mezomo Dam and Salso Stream. For biomonitoring, the free-living nematode Caenorhabditis elegans was used, which were exposed for 24 h. We did not find any significant alteration in physicochemical parameters. In the pre- and post-pesticides' samples we observed a residual presence of three pesticides (tebuconazole, imazethapyr, and clomazone) and metals which levels were above the recommended (As, Hg, Fe, and Mn). Exposure to both pre- and post-pesticides' samples impaired C. elegans reproduction and post-pesticides samples reduced worms' survival rate and lifespan. PCA analysis indicated that the presence of metals and pesticides are important variables that impacted C. elegans biological endpoints. Our data demonstrates that Uruguay River and two affluents are contaminated independent whether before or after pesticides' application season. In addition, it reinforces the usefulness of biological indicators, since simple physicochemical analyses are not sufficient to attest water quality and ecological safety.},
  language  = {en}
}
@misc{BaeslerMichaelisStibolleretal.2021,
  author    = {Baesler, Jessica and Michaelis, Vivien and Stiboller, Michael and Haase, Hajo and Aschner, Michael and Schwerdtle, Tanja and Sturzenbaum, Stephen R. and Bornhorst, Julia},
  title     = {Nutritive manganese and zinc overdosing in aging c. elegans result in a metallothionein-mediated alteration in metal homeostasis},
  series = {Zweitver{\"o}ffentlichungen der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe},
  journal   = {Zweitver{\"o}ffentlichungen der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe},
  number    = {8},
  issn      = {1866-8372},
  doi       = {10.25932/publishup-51499},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-514995},
  pages     = {13},
  year      = {2021},
  abstract  = {Manganese (Mn) and zinc (Zn) are not only essential trace elements, but also potential exogenous risk factors for various diseases. Since the disturbed homeostasis of single metals can result in detrimental health effects, concerns have emerged regarding the consequences of excessive exposures to multiple metals, either via nutritional supplementation or parenteral nutrition. This study focuses on Mn-Zn-interactions in the nematode Caenorhabditis elegans (C. elegans) model, taking into account aspects related to aging and age-dependent neurodegeneration.},
  language  = {en}
}
@article{BaeslerMichaelisStibolleretal.2021,
  author    = {Baesler, Jessica and Michaelis, Vivien and Stiboller, Michael and Haase, Hajo and Aschner, Michael and Schwerdtle, Tanja and Sturzenbaum, Stephen R. and Bornhorst, Julia},
  title     = {Nutritive manganese and zinc overdosing in aging c. elegans result in a metallothionein-mediated alteration in metal homeostasis},
  series = {Molecular Nutrition and Food Research},
  volume    = {65},
  journal   = {Molecular Nutrition and Food Research},
  number    = {8},
  publisher = {Wiley-VCH GmbH},
  address   = {Weinheim},
  issn      = {1613-4133},
  doi       = {10.1002/mnfr.202001176},
  pages     = {1 -- 11},
  year      = {2021},
  abstract  = {Manganese (Mn) and zinc (Zn) are not only essential trace elements, but also potential exogenous risk factors for various diseases. Since the disturbed homeostasis of single metals can result in detrimental health effects, concerns have emerged regarding the consequences of excessive exposures to multiple metals, either via nutritional supplementation or parenteral nutrition. This study focuses on Mn-Zn-interactions in the nematode Caenorhabditis elegans (C. elegans) model, taking into account aspects related to aging and age-dependent neurodegeneration.},
  language  = {en}
}
@misc{WinkelbeinerWandtEbertetal.2020,
  author    = {Winkelbeiner, Nicola Lisa and Wandt, Viktoria Klara Veronika and Ebert, Franziska and Lossow, Kristina and Bankoglu, Ezgi E. and Martin, Maximilian and Mangerich, Aswin and Stopper, Helga and Bornhorst, Julia and Kipp, Anna Patricia and Schwerdtle, Tanja},
  title     = {A Multi-Endpoint Approach to Base Excision Repair Incision Activity Augmented by PARylation and DNA Damage Levels in Mice},
  series = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe},
  journal   = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe},
  number    = {1021},
  issn      = {1866-8372},
  doi       = {10.25932/publishup-48483},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-484831},
  pages     = {21},
  year      = {2020},
  abstract  = {Investigation of processes that contribute to the maintenance of genomic stability is one crucial factor in the attempt to understand mechanisms that facilitate ageing. The DNA damage response (DDR) and DNA repair mechanisms are crucial to safeguard the integrity of DNA and to prevent accumulation of persistent DNA damage. Among them, base excision repair (BER) plays a decisive role. BER is the major repair pathway for small oxidative base modifications and apurinic/apyrimidinic (AP) sites. We established a highly sensitive non-radioactive assay to measure BER incision activity in murine liver samples. Incision activity can be assessed towards the three DNA lesions 8-oxo-2'-deoxyguanosine (8-oxodG), 5-hydroxy-2'-deoxyuracil (5-OHdU), and an AP site analogue. We applied the established assay to murine livers of adult and old mice of both sexes. Furthermore, poly(ADP-ribosyl)ation (PARylation) was assessed, which is an important determinant in DDR and BER. Additionally, DNA damage levels were measured to examine the overall damage levels. No impact of ageing on the investigated endpoints in liver tissue were found. However, animal sex seems to be a significant impact factor, as evident by sex-dependent alterations in all endpoints investigated. Moreover, our results revealed interrelationships between the investigated endpoints indicative for the synergetic mode of action of the cellular DNA integrity maintaining machinery.},
  language  = {en}
}
@article{BornhorstEbertMeyeretal.2020,
  author    = {Bornhorst, Julia and Ebert, Franziska and Meyer, S{\"o}ren and Ziemann, Vanessa and Xiong, Chan and Guttenberger, Nikolaus and Raab, Andrea and Baesler, Jessica and Aschner, Michael and Feldmann, J{\"o}rg and Francesconi, Kevin and Raber, Georg and Schwerdtle, Tanja},
  title     = {Toxicity of three types of arsenolipids},
  series = {Metallomics},
  volume    = {12},
  journal   = {Metallomics},
  number    = {5},
  publisher = {Oxford University Press},
  address   = {Cambridge},
  issn      = {1756-591X},
  doi       = {https://doi.org/10.1039/d0mt00039f},
  pages     = {794 -- 798},
  year      = {2020},
  abstract  = {Although fish and seafood are well known for their nutritional benefits, they contain contaminants that might affect human health. Organic lipid-soluble arsenic species, so called arsenolipids, belong to the emerging contaminants in these food items; their toxicity has yet to be systematically studied. Here, we apply the in vivo model Caenorhabditis elegans to assess the effects of two arsenic-containing hydrocarbons (AsHC), a saturated arsenic-containing fatty acid (AsFA), and an arsenic-containing triacylglyceride (AsTAG) in a whole organism. Although all arsenolipids were highly bioavailable in Caenorhabditis elegans, only the AsHCs were substantially metabolized to thioxylated or shortened metabolic products and induced significant toxicity, affecting both survival and development. Furthermore, the AsHCs were several fold more potent as compared to the toxic reference arsenite. This study clearly indicates the need for a full hazard identification of subclasses of arsenolipids to assess whether they pose a risk to human health.},
  language  = {en}
}
@article{NicolaiBaeslerAschneretal.2020,
  author    = {Nicolai, Merle Marie and Baesler, Jessica and Aschner, Michael and Schwerdtle, Tanja and Bornhorst, Julia},
  title     = {Consequences of manganese overload in C. elegans},
  series = {Naunyn-Schmiedeberg's archives of pharmacology / ed. for the Deutsche Gesellschaft f{\"u}r Experimentelle und Klinische Pharmakologie und Toxikologie},
  volume    = {393},
  journal   = {Naunyn-Schmiedeberg's archives of pharmacology / ed. for the Deutsche Gesellschaft f{\"u}r Experimentelle und Klinische Pharmakologie und Toxikologie},
  number    = {SUPPL 1},
  publisher = {Springer},
  address   = {New York},
  issn      = {0028-1298},
  doi       = {10.1007/s00210-020-01828-y},
  pages     = {9 -- 9},
  year      = {2020},
  language  = {en}
}