@misc{Vicente2011, author = {Vicente, Luis}, title = {{\´A}ngel J. Gallego, Phase theory}, series = {Postprints der Universit{\"a}t Potsdam : Humanwissenschaftliche Reihe}, journal = {Postprints der Universit{\"a}t Potsdam : Humanwissenschaftliche Reihe}, number = {539}, issn = {1866-8364}, doi = {10.25932/publishup-41311}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-413116}, pages = {719 -- 724}, year = {2011}, language = {en} } @phdthesis{Hoernke2011, author = {H{\"o}rnke, Maria}, title = {ß-Sheet formation of amyloidogenic model peptides at hydrophobic-hydrophilic interfaces}, address = {Potsdam}, pages = {107, XXI S.}, year = {2011}, language = {en} } @article{LilieBaerKettneretal.2011, author = {Lilie, Hauke and Baer, Dorit and Kettner, Karina and Weininger, Ulrich and Balbach, Jochen and Naumann, Manfred and Mueller, Eva-Christina and Otto, Albrecht and Gast, Klaus and Golbik, Ralph and Kriegel, Thomas}, title = {Yeast hexokinase isoenzyme ScHxk2 stability of a two-domain protein with discontinuous domains}, series = {Protein engineering design \& selection}, volume = {24}, journal = {Protein engineering design \& selection}, number = {1-2}, publisher = {Oxford Univ. Press}, address = {Oxford}, issn = {1741-0126}, doi = {10.1093/protein/gzq098}, pages = {79 -- 87}, year = {2011}, abstract = {The hexokinase isoenzyme 2 of Saccharomyces cerevisiae (ScHxk2) represents an archetype of a two-domain protein with the active site located in a cleft between the two domains. Binding of the substrate glucose results in a rigid body movement of the two domains leading to a cleft closure of the active site. Both domains of this enzyme are composed of discontinuous peptide sequences. This structural feature is reflected in the stability and folding of the ScHxk2 protein. Structural transitions induced by urea treatment resulted in the population of a thermodynamically stable folding intermediate, which, however, does not correspond to a molecule with one domain folded and the other unfolded. As demonstrated by different spectroscopic techniques, both domains are structurally affected by the partial denaturation. The intermediate possesses only 40\% of the native secondary structural content and a substantial increase in the Stokes radius as judged by circular dichroism and dynamic light scattering analyses. One-dimensional H-1 NMR data prove that all tryptophan residues are in a non-native environment in the intermediate, indicating substantial changes in the tertiary structure. Still, the intermediate possesses quite a high stability for a transition intermediate of about Delta G = -22 kJ mol(-1).}, language = {en} } @article{LilieBaerKettneretal.2011, author = {Lilie, Hauke and B{\"a}r, Dorit and Kettner, Karina and Weininger, Ulrich and Balbach, Jochen and Naumann, Manfred and M{\"u}ller, Eva-Christina and Otto, Albrecht and Gast, Klaus and Golbik, Ralph}, title = {Yeast hexokinase isoenzyme ScHxk2 : stability of a two-domain protein with discontinuous domains}, issn = {0269-2139}, year = {2011}, abstract = {The hexokinase isoenzyme 2 of Saccharomyces cerevisiae (ScHxk2) represents an archetype of a two-domain protein with the active site located in a cleft between the two domains. Binding of the substrate glucose results in a rigid body movement of the two domains leading to a cleft closure of the active site. Both domains of this enzyme are composed of discontinuous peptide sequences. This structural feature is reflected in the stability and folding of the ScHxk2 protein. Structural transitions induced by urea treatment resulted in the population of a thermodynamically stable folding intermediate, which, however, does not correspond to a molecule with one domain folded and the other unfolded. As demonstrated by different spectroscopic techniques, both domains are structurally affected by the partial denaturation. The intermediate possesses only 40\% of the native secondary structural content and a substantial increase in the Stokes radius as judged by circular dichroism and dynamic light scattering analyses. One-dimensional 1H NMR data prove that all tryptophan residues are in a non-native environment in the intermediate, indicating substantial changes in the tertiary structure. Still, the intermediate possesses quite a high stability for a transition intermediate of about ;G = ;22 kJ mol;1.}, language = {en} } @article{RedelbergerSedukGenestetal.2011, author = {Redelberger, David and Seduk, Farida and Genest, Olivier and Mejean, Vincent and Leimk{\"u}hler, Silke and Iobbi-Nivol, Chantal}, title = {YcdY Protein of Escherichia coli, an Atypical Member of the TorD Chaperone Family}, series = {Journal of bacteriology}, volume = {193}, journal = {Journal of bacteriology}, number = {23}, publisher = {American Society for Microbiology}, address = {Washington}, issn = {0021-9193}, doi = {10.1128/JB.05927-11}, pages = {6512 -- 6516}, year = {2011}, abstract = {The TorD family of specific chaperones is divided into four subfamilies dedicated to molybdoenzyme biogenesis and a fifth one, exemplified by YcdY of Escherichia coli, for which no defined partner has been identified so far. We propose that YcdY is the chaperone of YcdX, a zinc protein involved in the swarming motility process of E. coli, since YcdY interacts with YcdX and increases its activity in vitro.}, language = {en} } @article{KalimuthuLeimkuehlerBernhardt2011, author = {Kalimuthu, Palraj and Leimk{\"u}hler, Silke and Bernhardt, Paul V.}, title = {Xanthine dehydrogenase electrocatalysis autocatalysis and novel activity}, series = {The journal of physical chemistry : B, Condensed matter, materials, surfaces, interfaces \& biophysical chemistry}, volume = {115}, journal = {The journal of physical chemistry : B, Condensed matter, materials, surfaces, interfaces \& biophysical chemistry}, number = {11}, publisher = {American Chemical Society}, address = {Washington}, issn = {1520-6106}, doi = {10.1021/jp111809f}, pages = {2655 -- 2662}, year = {2011}, abstract = {The enzyme xanthine dehydrogenase (XDH) from the purple photosynthetic bacterium Rhodobacter capsulatus catalyzes the oxidation of hypoxanthine to xanthine and xanthine to uric acid as part of purine metabolism. The native electron acceptor is NAD(+) but herein we show that uric acid in its 2-electron oxidized form is able to act as an artificial electron acceptor from XDH in an electrochemically driven catalytic system. Hypoxanthine oxidation is also observed with the novel production of uric acid in a series of two consecutive 2-electron oxidation reactions via xanthine. XDH exhibits native activity in terms of its pH optimum and inhibition by allopurinol.}, language = {en} } @phdthesis{Wang2011, author = {Wang, Long}, title = {X-tracking the usage interest on web sites}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-51077}, school = {Universit{\"a}t Potsdam}, year = {2011}, abstract = {The exponential expanding of the numbers of web sites and Internet users makes WWW the most important global information resource. From information publishing and electronic commerce to entertainment and social networking, the Web allows an inexpensive and efficient access to the services provided by individuals and institutions. The basic units for distributing these services are the web sites scattered throughout the world. However, the extreme fragility of web services and content, the high competence between similar services supplied by different sites, and the wide geographic distributions of the web users drive the urgent requirement from the web managers to track and understand the usage interest of their web customers. This thesis, "X-tracking the Usage Interest on Web Sites", aims to fulfill this requirement. "X" stands two meanings: one is that the usage interest differs from various web sites, and the other is that usage interest is depicted from multi aspects: internal and external, structural and conceptual, objective and subjective. "Tracking" shows that our concentration is on locating and measuring the differences and changes among usage patterns. This thesis presents the methodologies on discovering usage interest on three kinds of web sites: the public information portal site, e-learning site that provides kinds of streaming lectures and social site that supplies the public discussions on IT issues. On different sites, we concentrate on different issues related with mining usage interest. The educational information portal sites were the first implementation scenarios on discovering usage patterns and optimizing the organization of web services. In such cases, the usage patterns are modeled as frequent page sets, navigation paths, navigation structures or graphs. However, a necessary requirement is to rebuild the individual behaviors from usage history. We give a systematic study on how to rebuild individual behaviors. Besides, this thesis shows a new strategy on building content clusters based on pair browsing retrieved from usage logs. The difference between such clusters and the original web structure displays the distance between the destinations from usage side and the expectations from design side. Moreover, we study the problem on tracking the changes of usage patterns in their life cycles. The changes are described from internal side integrating conceptual and structure features, and from external side for the physical features; and described from local side measuring the difference between two time spans, and global side showing the change tendency along the life cycle. A platform, Web-Cares, is developed to discover the usage interest, to measure the difference between usage interest and site expectation and to track the changes of usage patterns. E-learning site provides the teaching materials such as slides, recorded lecture videos and exercise sheets. We focus on discovering the learning interest on streaming lectures, such as real medias, mp4 and flash clips. Compared to the information portal site, the usage on streaming lectures encapsulates the variables such as viewing time and actions during learning processes. The learning interest is discovered in the form of answering 6 questions, which covers finding the relations between pieces of lectures and the preference among different forms of lectures. We prefer on detecting the changes of learning interest on the same course from different semesters. The differences on the content and structure between two courses leverage the changes on the learning interest. We give an algorithm on measuring the difference on learning interest integrated with similarity comparison between courses. A search engine, TASK-Moniminer, is created to help the teacher query the learning interest on their streaming lectures on tele-TASK site. Social site acts as an online community attracting web users to discuss the common topics and share their interesting information. Compared to the public information portal site and e-learning web site, the rich interactions among users and web content bring the wider range of content quality, on the other hand, provide more possibilities to express and model usage interest. We propose a framework on finding and recommending high reputation articles in a social site. We observed that the reputation is classified into global and local categories; the quality of the articles having high reputation is related with the content features. Based on these observations, our framework is implemented firstly by finding the articles having global or local reputation, and secondly clustering articles based on their content relations, and then the articles are selected and recommended from each cluster based on their reputation ranks.}, language = {en} } @article{GiewekemeyerKruegerKalbfleischetal.2011, author = {Giewekemeyer, K. and Krueger, S. P. and Kalbfleisch, S. and Bartels, Meike and Beta, Carsten and Salditt, T.}, title = {X-ray propagation microscopy of biological cells using waveguides as a quasipoint source}, series = {Physical review : A, Atomic, molecular, and optical physics}, volume = {83}, journal = {Physical review : A, Atomic, molecular, and optical physics}, number = {2}, publisher = {American Physical Society}, address = {College Park}, issn = {1050-2947}, doi = {10.1103/PhysRevA.83.023804}, pages = {7}, year = {2011}, abstract = {We have used x-ray waveguides as highly confining optical elements for nanoscale imaging of unstained biological cells using the simple geometry of in-line holography. The well-known twin-image problem is effectively circumvented by a simple and fast iterative reconstruction. The algorithm which combines elements of the classical Gerchberg-Saxton scheme and the hybrid-input-output algorithm is optimized for phase-contrast samples, well-justified for imaging of cells at multi-keV photon energies. The experimental scheme allows for a quantitative phase reconstruction from a single holographic image without detailed knowledge of the complex illumination function incident on the sample, as demonstrated for freeze-dried cells of the eukaryotic amoeba Dictyostelium discoideum. The accessible resolution range is explored by simulations, indicating that resolutions on the order of 20 nm are within reach applying illumination times on the order of minutes at present synchrotron sources.}, language = {en} } @article{ParkinBroosTownsleyetal.2011, author = {Parkin, E. R. and Broos, Patrick S. and Townsley, L. K. and Pittard, J. M. and Moffat, Anthony F. J. and Naze, Y. and Rauw, G. and Oskinova, Lida and Waldron, W. L.}, title = {X-RAY EMISSION FROM THE DOUBLE-BINARY OB-STAR SYSTEM QZ CAR (HD 93206)}, series = {ASTROPHYSICAL JOURNAL SUPPLEMENT SERIES}, volume = {194}, journal = {ASTROPHYSICAL JOURNAL SUPPLEMENT SERIES}, number = {1}, publisher = {IOP PUBLISHING LTD}, address = {BRISTOL}, issn = {0067-0049}, doi = {10.1088/0067-0049/194/1/8}, pages = {11}, year = {2011}, abstract = {X-ray observations of the double-binary OB-star system QZ Car (HD 93206) obtained with the Chandra X-ray Observatory over a period of roughly 2 years are presented. The respective orbits of systems A (O9.7 I+b2 v, P-A = 21 days) and B (O8 III+o9 v, P-B = 6 days) are reasonably well sampled by the observations, allowing the origin of the X-ray emission to be examined in detail. The X-ray spectra can be well fitted by an attenuated three-temperature thermal plasma model, characterized by cool, moderate, and hot plasma components at kT similar or equal to 0.2, 0.7, and 2 keV, respectively, and a circumstellar absorption of similar or equal to 0.2 x 10(22) cm(-2). Although the hot plasma component could be indicating the presence of wind-wind collision shocks in the system, the model fluxes calculated from spectral fits, with an average value of similar or equal to 7x10(-13) erg s(-1) cm(-2), do not show a clear correlation with the orbits of the two constituent binaries. A semi-analytical model of QZ Car reveals that a stable momentum balance may not be established in either system A or B. Yet, despite this, system B is expected to produce an observed X-ray flux well in excess of the observations. If one considers the wind of the O8 III star to be disrupted by mass transfer, the model and observations are in far better agreement, which lends support to the previous suggestion of mass transfer in the O8 III+o9 v binary. We conclude that the X-ray emission from QZ Car can be reasonably well accounted for by a combination of contributions mainly from the single stars and the mutual wind-wind collision between systems A and B.}, language = {en} } @article{OskinovaHamannCassinellietal.2011, author = {Oskinova, Lida and Hamann, Wolf-Rainer and Cassinelli, Joseph P. and Brown, John C. and Todt, Helge Tobias}, title = {X-ray emission from massive stars with magnetic fields}, series = {Astronomische Nachrichten = Astronomical notes}, volume = {332}, journal = {Astronomische Nachrichten = Astronomical notes}, number = {9-10}, publisher = {Wiley-Blackwell}, address = {Malden}, issn = {0004-6337}, doi = {10.1002/asna.201111602}, pages = {988 -- 993}, year = {2011}, abstract = {We investigate the connections between the magnetic fields and the X-ray emission from massive stars. Our study shows that the X-ray properties of known strongly magnetic stars are diverse: while some comply to the predictions of the magnetically confined wind model, others do not. We conclude that strong, hard, and variable X-ray emission may be a sufficient attribute of magnetic massive stars, but it is not a necessary one. We address the general properties of X-ray emission from "normal" massive stars, especially the long standing mystery about the correlations between the parameters of X-ray emission and fundamental stellar properties. The recent development in stellar structure modeling shows that small-scale surface magnetic fields may be common. We suggest a "hybrid" scenario which could explain the X-ray emission from massive stars by a combination of magnetic mechanisms on the surface and shocks in the stellar wind. The magnetic mechanisms and the wind shocks are triggered by convective motions in sub-photospheric layers. This scenario opens the door for a natural explanation of the well established correlation between bolometric and X-ray luminosities.}, language = {en} }