@article{WanjikuYamamotoKlosseketal.2019,
  author    = {Wanjiku, Barbara and Yamamoto, Kenji and Klossek, Andre and Schumacher, Fabian and Pischon, Hannah and Mundhenk, Lars and Rancan, Fiorenza and Judd, Martyna M. and Ahmed, Muniruddin and Zoschke, Christian and Kleuser, Burkhard and R{\"u}hl, Eckart and Sch{\"a}fer-Korting, Monika},
  title     = {Qualifying X-ray and Stimulated Raman Spectromicroscopy for Mapping Cutaneous Drug Penetration},
  series = {Analytical chemistry},
  volume    = {91},
  journal   = {Analytical chemistry},
  number    = {11},
  publisher = {American Chemical Society},
  address   = {Washington},
  issn      = {0003-2700},
  doi       = {10.1021/acs.analchem.9b00519},
  pages     = {7208 -- 7214},
  year      = {2019},
  abstract  = {Research on topical drug delivery relies on reconstructed human skin (RHS) in addition to ex vivo human and animal skin, each with specific physiological features. Here, we compared the penetration of dexamethasone from an ethanolic hydroxyethyl cellulose gel into ex vivo human skin, murine skin, and RHS. For comprehensive insights into skin morphology and penetration enhancing mechanisms, scanning transmission X-ray microscopy (STXM), liquid chromatography tandem mass spectrometry (LC-MS/MS), and stimulated Raman spectromicroscopy (SRS) were combined. STXM offers high spatial resolution with label-free drug detection and is therefore sensitive to tissue damage. Despite differences in sample preparation and data analysis, the amounts of dexamethasone in RHS, detected and quantified by STXM and LC-MS/MS, were very similar and increased during the first 100 min of exposure. SRS revealed interactions between the gel and the stratum corneum or, more specifically, its protein and lipid structures. Similar to both types of ex vivo skin, higher protein-to-lipid ratios within the stratum corneum of RHS indicated reduced lipid amounts after 30 min of ethanol exposure. Extended ethanol exposure led to a continued reduction of lipids in the ex vivo matrixes, while protein integrity appeared to be compromised in RHS, which led to declining protein signals. In conclusion, LC-MS/MS proved the predictive capability of STXM for label-free drug detection. Combining STXM with SRS precisely dissected the penetration enhancing effects of ethanol. Further studies on topical drug delivery should consider the potential of these complementary techniques.},
  language  = {en}
}
@article{LuReichetzederPrehnetal.2018,
  author    = {Lu, Yong-Ping and Reichetzeder, Christoph and Prehn, Cornelia and von Websky, Karoline and Slowinski, Torsten and Chen, You-Peng and Yin, Liang-Hong and Kleuser, Burkhard and Yang, Xue-Song and Adamski, Jerzy and Hocher, Berthold},
  title     = {Fetal serum metabolites are independently associated with Gestational diabetes mellitus},
  series = {Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry and pharmacology},
  volume    = {45},
  journal   = {Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry and pharmacology},
  number    = {2},
  publisher = {Karger},
  address   = {Basel},
  issn      = {1015-8987},
  doi       = {10.1159/000487119},
  pages     = {625 -- 638},
  year      = {2018},
  abstract  = {Background/Aims: Gestational diabetes (GDM) might be associated with alterations in the metabolomic profile of affected mothers and their offspring. Until now, there is a paucity of studies that investigated both, the maternal and the fetal serum metabolome in the setting of GDM. Mounting evidence suggests that the fetus is not just passively affected by gestational disease but might play an active role in it. Metabolomic studies performed in maternal blood and fetal cord blood could help to better discern distinct fetal from maternal disease interactions. Methods: At the time of birth, serum samples from mothers and newborns (cord blood samples) were collected and screened for 163 metabolites utilizing tandem mass spectrometry. The cohort consisted of 412 mother/child pairs, including 31 cases of maternal GDM. Results: An initial non-adjusted analysis showed that eight metabolites in the maternal blood and 54 metabolites in the cord blood were associated with GDM. After Benjamini-Hochberg (BH) procedure and adjustment for confounding factors for GDM, fetal phosphatidylcholine acyl-alkyl C 32:1 and proline still showed an independent association with GDM. Conclusions: This study found metabolites in cord blood which were associated with GDM, even after adjustment for established risk factors of GDM. To the best of our knowledge, this is the first study demonstrating an independent association between fetal serum metabolites and maternal GDM. Our findings might suggest a potential effect of the fetal metabolome on maternal GDM. (c) 2018 The Author(s) Published by S. Karger AG, Basel},
  language  = {en}
}
@article{GiulbudagianYeallandHoenzkeetal.2018,
  author    = {Giulbudagian, Michael and Yealland, Guy and H{\"o}nzke, S. and Edlich, A. and Geisend{\"o}rfer, Birte and Kleuser, Burkhard and Hedtrich, Sarah and Calderon, Marcelo},
  title     = {Breaking the Barrier},
  series = {Theranostics},
  volume    = {8},
  journal   = {Theranostics},
  number    = {2},
  publisher = {Ivyspring International Publisher},
  address   = {Lake haven},
  issn      = {1838-7640},
  doi       = {10.7150/thno.21668},
  pages     = {450 -- 463},
  year      = {2018},
  abstract  = {Topical administration permits targeted, sustained delivery of therapeutics to human skin. Delivery to the skin, however, is typically limited to lipophilic molecules with molecular weight of < 500 Da, capable of crossing the stratum corneum. Nevertheless, there are indications protein delivery may be possible in barrier deficient skin, a condition found in several inflammatory skin diseases such as psoriasis, using novel nanocarrier systems. Methods: Water in water thermo-nanoprecipitation; dynamic light scattering; zeta potential measurement; nanoparticle tracking analysis; atomic force microscopy; cryogenic transmission electron microscopy; UV absorption; centrifugal separation membranes; bicinchoninic acid assay; circular dichroism; TNF alpha binding ELISA; inflammatory skin equivalent construction; human skin biopsies; immunohistochemistry; fluorescence microscopy; western blot; monocyte derived Langerhans cells; ELISA Results: Here, we report the novel synthesis of thermoresponsive nanogels (tNG) and the stable encapsulation of the anti-TNFa fusion protein etanercept (ETR) (similar to 150 kDa) without alteration to its structure, as well as temperature triggered release from the tNGs. Novel tNG synthesis without the use of organic solvents was conducted, permitting in situ encapsulation of protein during assembly, something that holds great promise for easy manufacture and storage. Topical application of ETR loaded tNGs to inflammatory skin equivalents or tape striped human skin resulted in efficient ETR delivery throughout the SC and into the viable epidermis that correlated with clear anti-inflammatory effects. Notably, effective ETR delivery depended on temperature triggered release following topical application. Conclusion: Together these results indicate tNGs hold promise as a biocompatible and easy to manufacture vehicle for stable protein encapsulation and topical delivery into barrier-deficient skin.},
  language  = {en}
}
@inproceedings{FrombachRancanFleigeetal.2015,
  author    = {Frombach, Janna and Rancan, Fiorenza and Fleige, Emanuel and Haag, Rainer and Schumacher, Frank and Kleuser, Burkhard and Yamamoto, Kenji and R{\"u}hl, Eckart and Blume-Peytavi, Ulrike and Vogt, Annika},
  title     = {Skin penetration and dexamethasone release from polymer nanoparticles in ex vivo human skin},
  series = {The journal of investigative dermatology},
  volume    = {135},
  booktitle = {The journal of investigative dermatology},
  publisher = {Nature Publ. Group},
  address   = {New York},
  issn      = {0022-202X},
  pages     = {S52 -- S52},
  year      = {2015},
  language  = {en}
}
@inproceedings{ArltSchwiebsPfarretal.2014,
  author    = {Arlt, Olga and Schwiebs, Anja and Pfarr, Kathrin and Ranglack, Annika and Bouzas, Ferreiros Nerea and Schreiber, Yannick and Neuber, Corinna and Kleuser, Burkhard and Pfeilschifter, Josef M. and Radeke, Heinfried H.},
  title     = {Dynamic interaction between sphingolipid enzymes, S1P and inflammatory cytokine regulation in dendritic cells},
  series = {NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY},
  volume    = {387},
  booktitle = {NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY},
  publisher = {Springer},
  address   = {New York},
  issn      = {0028-1298},
  pages     = {S91 -- S91},
  year      = {2014},
  language  = {en}
}