@article{KoebnickWagnerThieleckeetal.2005, author = {Koebnick, Corinna and Wagner, K. and Thielecke, F. and Dieter, G. and Hohne, A. and Franke, A. and Garcia, Ada and Meyer, H. and Hoffmann, I. and Leitzmann, P. and Trippo, U. and Zunft, Hans-Joachim Franz}, title = {An easy-to-use semiquantitative food record validated for energy intake by using doubly labelled water technique}, issn = {0954-3007}, year = {2005}, abstract = {Background: Estimating dietary intake is important for both epidemiological and clinical studies, but often lacks accuracy. Objective: To investigate the accuracy and validity of energy intake estimated by an easy-to-use semiquantitative food record (EISQFR) compared to total energy expenditure ( TEE) estimated by doubly labelled water technique (EEDLW). Design: TEE was measured in 29 nonobese subjects using the doubly labelled water method over a period of 14 days. Within this period, subjects reported their food consumption by a newly developed semiquantitative food record for 4 consecutive days. Energy intake was calculated using the German Food Code and Nutrition Data Base BLS II.3. Results: A good correlation was observed between EISQFR and EEDLW (r = 0.685, P<0.001). The mean difference between EISQFR and EEDLW was - 1.7 +/- 2.6 MJ/ day ( - 14 +/- 21\%, P = 0.002). An underestimation of EISQFR <10\% was observed in nine subjects (31\%), of 10 - 20\% in six subjects (21\%), and of >20\% in nine subjects (31\%). In five subjects (17\%), an overestimation of EISQFR was observed. Conclusions: The easy-to-use semiquantitative food record provided good estimates of EI in free-living and nonobese adults without prior detailed verbal instructions. The presented food record has limitations regarding accuracy at the individual level}, language = {en} } @article{GarciaWagnerEinigetal.2006, author = {Garcia, Ada and Wagner, Karen and Einig, Christiana and Trippo, Ulrike and Koebnick, Corinna and Zunft, Hans-Joachim Franz}, title = {Evaluation of body fat changes during weight loss by using improved anthropometric predictive equations}, issn = {0250-6807}, doi = {10.1159/000092601}, year = {2006}, abstract = {Background/Aim: Skinfold-based equations are widely used to evaluate body fat (BF), but over-/underestimation is often reported. We evaluate the capacity of improved skinfold-based equations to estimate BF changes during weight reduction and compare them against well-established equations. Methods: Overweight adults (n = 44) participated in a 4- month weight reduction intervention. Dual-energy X-ray absorptiometry (DXA) and anthropometric measurements were taken at baseline and after intervention. The BF\% was calculated using Garcia, Peterson, and Durnin and Womersley (DW) equations. Results: Baseline and postintervention BF\% measured by DXA correlated highest with BF\% predicted according to Garcia (r = 0.934 and r = 0.948, respectively), followed by Peterson (r = 0.941 and r = 0.932, respectively) and DW (r = 0.557 and r = 0.402, respectively); only a slight systematic error in overestimating the BF\% was observed in estimates according to Garcia (r = 0.147 and r = 0.104, respectively; p < 0.001), while increasing errors occurred using the Peterson (r = 0.624 and r = 0.712, respectively; p < 0.001) and DW (r = 0.767 and r = 0.769, respectively; p < 0.001) equations. Moderate correlations between BF changes (kg) measured by DXA and predicted by DW (r = 0.7211), Peterson (r = 0.697), and Garcia (r = 0.645) were observed. Conclusion: Improved skin-fold equations cannot accurately measure changes in BF after weight reduction}, language = {en} } @article{RuehlHamscherGarciaetal.2004, author = {R{\"u}hl, Ralph and Hamscher, Gerd and Garcia, Ada and Nau, Heinz and Schweigert, Florian J.}, title = {Identification of 14-hydroxy-retro-retinol and 4-hydroxy-retinol as endogenous retinoids in rats throughout neonatal development}, issn = {0024-3205}, year = {2004}, abstract = {14-Hydroxy-retro-retinol was previously described as an in vivo and in vitro metabolite of retinol. Furthermore, the retinoid 4-hydroxy-retinol was identified as an endogenous occurring retinoid in the amphibian organism and an in vitro metabolite of retinol. We describe in the present study that 14-hydroxy-retro-retinol and 4-hydroxy- retinol are present in normal neonatal rat serum as endogenous occurring retinoids in normal non-vitamin A supplemented mammals (rats). Both retinoids were detected in serum and liver of neonatal rats at days 3 and 11 after birth. The respective concentrations at day 11 after birth were 41.8 +/- 2.8 ng/ml (serum)/ 104 +/- 6 ng/g (liver) for 4-hydroxy- retinol and 23 +/- 4.6 ng/ml (serum)/ 285 +/- 5 ng/g (liver) for 14-hydroxy-retro-retinol. Both retinoids could not be detected in adult rat serum and liver. From our experiments important physiological functions of these retinoids during postnatal development could be postulated. (C) 2004 Elsevier Inc. All rights reserved}, language = {en} } @article{GarciaWagnerHothornetal.2005, author = {Garcia, Ada and Wagner, Karen and Hothorn, T. and Koebnick, Corinna and Zunft, Hans-Joachim Franz and Trippo, Ulrike}, title = {Improved prediction of body fat by measuring skinfold thickness, circumferences, and bone breadths}, issn = {1071-7323}, year = {2005}, abstract = {Objective: To develop improved predictive regression equations for body fat content derived from common anthropometric measurements. Research Methods and Procedures: 117 healthy German subjects, 46 men and 71 women, 26 to 67 years of age, from two different studies were assigned to a validation and a cross-validation group. Common anthropornetric measurements and body composition by DXA were obtained. Equations using anthropometric measurements predicting body fat mass (BFM) with DXA as a reference method were developed using regression models. Results: The final best predictive sex-specific equations combining skinfold thicknesses (SF), circumferences, and bone breadth measurements were as follows: BFMNew (kg) for men = -40.750 + {(0.397 x waist circumference) + [6.568 x (log triceps SF + log subscapular SF + log abdominal SF)]} and BFMNew (kg) for women = -75.231 + {(0.512 x hip circumference) + [8.889 x (log chin SF + log triceps SF + log subscapular SF)] + (1.905 x knee breadth)}. The estimates of BFM from both validation and cross-validation had an excellent correlation, showed excellent correspondence to the DXA estimates, and showed a negligible tendency to underestimate percent body fat in subjects with higher BFM compared with equations using a two-compartment (Durnin and Womersley) or a four-compartment (Peterson) model as the reference method. Discussion: Combining skinfold thicknesses with circumference and/or bone breadth measures provide a more precise prediction of percent body fat in comparison with established SF equations. Our equations are recommended for use in clinical or epidemiological settings in populations with similar ethnic background}, language = {en} } @article{RuehlGarciaSchweigertetal.2004, author = {R{\"u}hl, Ralph and Garcia, Ada and Schweigert, Florian J. and Worm, Margitta}, title = {Modulation of cytokine production by low and high retinoid diet in ovalbumin-sensitized mice}, year = {2004}, abstract = {Retinoids modulate many physiological processes such as the differentiation and growth of different cell types. including cells from the immune system. We have previously shown that retinoids modulate IgE production in vitro and in vivo. In the present study we investigated the effects of retinoids in non-sensitized and ovalbumin-sensitized mice that were fed for 11 weeks with three different vitamin A (VA) diets: a) VA-deficiency diet, b) base diet, and c) base diet supplemented with 0.5\% all-trans-retinoic acid (ATRA). Phorbol-myristate-acetate (PMA)/ionomycin-stimulated SMC (splenic mononuclear cells) from mice fed with ATRA and the vitamin A-deficient diet group showed increased interleukin-4 (IL-4) responses in non-sensitized mice. After ovalbumin sensitization in the VA-deficient and the ATRA supplementation diet groups, no significant effects on IL-4 production were observed. By contrast, gamma interferon (IFN-gamma production from PMA/ionomycin-stimulated SMC was enhanced in the VA-deficient diet group in ovalbumin-sensitized mice, and also in non-sensitized mice compared to the base and the ATRA-supplemented diet group. The data indicate that VA and retinoid content in a diet influences the cytokine response in non-sensitized and also ovalbumin-sensitized mice. Therefore these molecules may serve as active modulators of cytokine production in vivo that are responsible for the induction and persistence of atopic diseases}, language = {en} } @book{RuehlGarciaHerzetal.2001, author = {R{\"u}hl, Ralph and Garcia, Ada and Herz, U. and Schweigert, Florian J. and Renz, H.}, title = {Postnataler Einfluss von Retinoiden auf die Ontogenese des Immunsystems der Maus}, year = {2001}, language = {de} } @article{GarciaRuhlSchweigert2005, author = {Garcia, Ada and Ruhl, R. and Schweigert, Florian J.}, title = {Retinoid concentrations in the mouse during postnatal development and after maternal vitamin a supplementation}, issn = {0250-6807}, year = {2005}, abstract = {Background: Vitamin A (VA) and its derivates (retinoids) are important nutritional substances, which mediate their biological activity mainly via nuclear retinoid receptors. Maternal VA intake during lactation influences the VA content in milk and the VA status of the progeny. We investigated the effects of maternal supplementation during lactation and direct supplementation to the pups after weaning on the retinoid concentration in serum and liver of neonatal mice using high doses of VA. Methods: Dams were fed a basal (4,500 retinol equivalents/ kg diet) or a VA- supplemented (324,000 retinol equivalents/ kg diet) diet during lactation. Pups kept receiving the same diet after weaning. Serum and liver samples of the pups were collected during lactation at days 1, 3, 5, 7, and 14 and post-weaning at days 21 and 65 after birth. Samples were analysed for retinoids by high-performance liquid chromatography. Results: Maternal VA supplementation resulted in significantly higher concentrations of retinol, retinyl palmitate and retinyl stearate in serum of mice neonates at days 5, 7, 14, 21 and 65 after birth in comparison to the basal diet, whereas significantly higher concentrations were observed in liver at days 5, 14, 21 and 65 after birth. At day 7 after birth, a decrease in the liver retinoid concentrations occurred in the VA-supplemented diet. Conclusion: Our results show for the first time that supplementation with high doses of VA during the lactation period in mice can affect serum retinol concentrations in the neonates and report that day 7 after birth is a critical time in the tissue distribution of retinoids during postnatal development. Copyright (C) 2005 S. Karger AG, Basel}, language = {en} } @article{GarciaRuehlHerzetal.2003, author = {Garcia, Ada and R{\"u}hl, Ralph and Herz, U. and Koebnick, Corinna and Schweigert, Florian J. and Worm, Margitta}, title = {Retinoid- and carotenoid-enriched diets influence the ontogenesis of the immune system in mice}, year = {2003}, language = {en} } @article{KoebnickWagnerThieleckeetal.2005, author = {Koebnick, Corinna and Wagner, K. and Thielecke, F. and Moeseneder, Jutta and Hoehne, A. and Franke, A. and Meyer, H. and Garcia, Ada and Trippo, U. and Zunft, Hans-Joachim Franz}, title = {Validation of a simplified physical activity record by doubly labeled water technique}, issn = {0307-0565}, year = {2005}, abstract = {INTRODUCTION: For obtaining reliable information about physical activity in epidemiological studies, validated and easy-to-use instruments are required. Therefore, a new simplified physical activity record based on 15-min recording intervals was developed and validated. SUBJECTS: Nonobese volunteers (n = 31). MEASUREMENTS: Physical activity was recorded over a 7-day period without detailed instructions. Energy expenditure was calculated (EEsPAR) and compared to energy expenditure measured by doubly labelled water technique (EEDLW). RESULTS: A good agreement between EEsPAR (12.1 +/ 3.0) and EEDLW (11.7 +/- 3.3) with a mean difference of 0.33 +/- 1.55 MJ (r = 0.880, P < 0.001) was observed. The absolute difference between EEsPAR and EEDLW was <10\% in 65\% of the subjects. The difference between EEsPAR and EEDLW was independent of gender, age, body weight, and body mass index. A weak positive association between the difference and total body fat was observed (r = 0.618, P < 0.001), suggesting a slight tendency to overestimate EEsPAR with increasing total body fat. CONCLUSION: The new simplified physical activity protocol needs no detailed instructions, provides valid estimates of physical activity in nonobese free-living adults and can be used in epidemiological studies to assess total daily energy expenditure and physical activity level}, language = {en} }