@article{StanglmairNeubrechPacholski2018,
  author    = {Stanglmair, Christoph and Neubrech, Frank and Pacholski, Claudia},
  title     = {Chemical routes to surface enhanced infrared absorption (SEIRA) substrates},
  series = {Zeitschrift f{\"u}r physikalische Chemie : international journal of research in physical chemistry and chemical physics},
  volume    = {232},
  journal   = {Zeitschrift f{\"u}r physikalische Chemie : international journal of research in physical chemistry and chemical physics},
  number    = {9-11},
  publisher = {De Gruyter},
  address   = {Berlin},
  issn      = {0942-9352},
  doi       = {10.1515/zpch-2018-1132},
  pages     = {1527 -- 1539},
  year      = {2018},
  abstract  = {Bottom-up strategies for fabricating SEIRA substrates are presented. For this purpose, wet-chemically prepared gold nanoparticles are coated with a polystyrene shell and subsequently self-assembled into different nanostructures such as quasi-hexagonally ordered gold nanoparticle monolayers, double layers, and honeycomb structures. Furthermore elongated gold nanostructures are obtained by sintering of gold nanoparticle double layers. The optical properties of these different gold nanostructures are directly connected to their morphology and geometrical arrangement - leading to surface plasmon resonances from the visible to the infrared wavelength range. Finally, SEIRA enhancement factors are determined. Gold nanoparticle double layers show the best performance as SEIRA substrates.},
  language  = {en}
}
@article{FallahStanglmairPacholskietal.2016,
  author    = {Fallah, Mohammad A. and Stanglmair, Christoph and Pacholski, Claudia and Hauser, Karin},
  title     = {Devising Self-Assembled-Monolayers for Surface-Enhanced Infrared Spectroscopy of pH-Driven Poly-L-lysine Conformational Changes},
  series = {Langmuir},
  volume    = {32},
  journal   = {Langmuir},
  publisher = {American Chemical Society},
  address   = {Washington},
  issn      = {0743-7463},
  doi       = {10.1021/acs.langmuir.6b01742},
  pages     = {7356 -- 7364},
  year      = {2016},
  abstract  = {Surface-enhanced infrared absorption spectroscopy (SEIRA) is applied to study protein conformational changes. In general, the appropriate functionalization of metal surfaces with biomolecules remains a challenge if the conformation and activity of the biomolecule shall be preserved. Here we present a SEIRA study to monitor pH-induced conformational changes of poly-L lysine (PLL) covalently bound to a thin gold layer via self assembled monolayers (SAMs). We demonstrate that the composition of the SAM is crucial. A SAM of 11-mercaptoundecanonic acid (MUA) can link PLL to the gold layer, but pH-driven conformational transitions were hindered compared to poly-L lysine in solution. To address this problem, we devised a variety of SAMs, i.e., mixed SAMs of MUA with either octanethiol (OT) or 11-mercapto-1-undecanol (MUoL) and furthermore SAMs of MT(PEG)(4) and NHS-PEG(10k)-SH. These mixed SAMs modify the surface properties by changing the polarity and the morphology of the surface present to nearby PLL molecules. Our experiments reveal that mixed SAMs of MUA-MUoL and SAMs of NHS-PEG(10k)-SH-MT(PEG)(4) are suitable to monitor pH-driven conformational changes of immobilized PLL. These SAMs might be applicable for chemoselective protein immobilization in general.},
  language  = {en}
}