@article{NumbergerRiedelMcEwenetal.2019,
  author    = {Numberger, Daniela and Riedel, Thomas and McEwen, Gayle and N{\"u}bel, Ulrich and Frentrup, Martinique and Schober, Isabel and Bunk, Boyke and Spr{\"o}er, Cathrin and Overmann, J{\"o}rg and Grossart, Hans-Peter and Greenwood, Alex D.},
  title     = {Genomic analysis of three Clostridioides difficile isolates from urban water sources},
  series = {Anaerobe},
  volume    = {56},
  journal   = {Anaerobe},
  publisher = {Elsevier},
  address   = {Oxford},
  issn      = {1075-9964},
  doi       = {10.1016/j.anaerobe.2019.01.002},
  pages     = {22 -- 26},
  year      = {2019},
  abstract  = {We investigated inflow of a wastewater treatment plant and sediment of an urban lake for the presence of Clostridioides difficile by cultivation and PCR. Among seven colonies we sequenced the complete genomes of three: two non-toxigenic isolates from wastewater and one toxigenic isolate from the urban lake. For all obtained isolates, a close genomic relationship with human-derived isolates was observed. (C) 2019 Elsevier Ltd. All rights reserved.},
  language  = {en}
}
@article{NumbergerDreierVullioudetal.2019,
  author    = {Numberger, Daniela and Dreier, Carole and Vullioud, Colin and Gabriel, G{\"u}lsah and Greenwood, Alex D. and Grossart, Hans-Peter},
  title     = {Recovery of influenza a viruses from lake water and sediments by experimental inoculation},
  series = {PLoS one},
  volume    = {14},
  journal   = {PLoS one},
  number    = {5},
  publisher = {PLoS},
  address   = {San Fransisco},
  issn      = {1932-6203},
  doi       = {10.1371/journal.pone.0216880},
  pages     = {13},
  year      = {2019},
  abstract  = {Influenza A viruses (IAV) are zoonotic pathogens relevant to human, domestic animal and wildlife health. Many avian IAVs are transmitted among waterfowl via a faecal-oral-route. Therefore, environmental water where waterfowl congregate may play an important role in the ecology and epidemiology of avian IAV. Water and sediment may sustain and transmit virus among individuals or species. It is unclear at what concentrations waterborne viruses are infectious or remain detectable. To address this, we performed lake water and sediment dilution experiments with varying concentrations or infectious doses of four IAV strains from seal, turkey, duck and gull. To test for infectivity of the IAV strains in a concentration dependent manner, we applied cultivation to specific pathogen free (SPF) embryonated chicken eggs and Madin-Darby Canine Kidney (MDCK) cells. IAV recovery was more effective from embryonated chicken eggs than MDCK cells for freshwater lake dilutions, whereas, MDCK cells were more effective for viral recovery from sediment samples. Low infectious dose (1 PFU/200 mu L) was sufficient in most cases to detect and recover IAV from lake water dilutions. Sediment required higher initial infectious doses (>= 100 PFU/200 mu L).},
  language  = {en}
}
@misc{NumbergerDreierVullioudetal.2019,
  author    = {Numberger, Daniela and Dreier, Carola and Vullioud, Colin and Gabriel, Guelsah and Greenwood, Alex D. and Grossart, Hans-Peter},
  title     = {Correction: Recovery of influenza A viruses from lake water and sediments by experimental inoculation (vol 14, e0216880, 2019)},
  series = {PLoS one},
  volume    = {14},
  journal   = {PLoS one},
  number    = {6},
  publisher = {PLoS},
  address   = {San Fransisco},
  issn      = {1932-6203},
  doi       = {10.1371/journal.pone.0218882},
  pages     = {1},
  year      = {2019},
  language  = {en}
}
@article{NumbergerGanzertZoccaratoetal.2019,
  author    = {Numberger, Daniela and Ganzert, Lars and Zoccarato, Luca and M{\"u}hldorfer, Kristin and Sauer, Sascha and Grossart, Hans-Peter and Greenwood, Alex D.},
  title     = {Characterization of bacterial communities in wastewater with enhanced taxonomic resolution by full-length 16S rRNA sequencing},
  series = {Scientific reports},
  volume    = {9},
  journal   = {Scientific reports},
  publisher = {Nature Publ. Group},
  address   = {London},
  issn      = {2045-2322},
  doi       = {10.1038/s41598-019-46015-z},
  pages     = {14},
  year      = {2019},
  abstract  = {Wastewater treatment is crucial to environmental hygiene in urban environments. However, wastewater treatment plants (WWTPs) collect chemicals, organic matter, and microorganisms including pathogens and multi-resistant bacteria from various sources which may be potentially released into the environment via WWTP effluent. To better understand microbial dynamics in WWTPs, we characterized and compared the bacterial community of the inflow and effluent of a WWTP in Berlin, Germany using full-length 16S rRNA gene sequences, which allowed for species level determination in many cases and generally resolved bacterial taxa. Significantly distinct bacterial communities were identified in the wastewater inflow and effluent samples. Dominant operational taxonomic units (OTUs) varied both temporally and spatially. Disease associated bacterial groups were efficiently reduced in their relative abundance from the effluent by the WWTP treatment process, except for Legionella and Leptospira species which demonstrated an increase in relative proportion from inflow to effluent. This indicates that WWTPs, while effective against enteric bacteria, may enrich and release other potentially pathogenic bacteria into the environment. The taxonomic resolution of full-length 16S rRNA genes allows for improved characterization of potential pathogenic taxa and other harmful bacteria which is required to reliably assess health risk.},
  language  = {en}
}