@article{KrylovAdamzigWalteretal.2006,
  author    = {Krylov, Andrey. V. and Adamzig, H. and Walter, A. D. and Loechel, B. and Kurth, E. and Pulz, O. and Szeponik, Jan and Wegerich, Franziska and Lisdat, Fred},
  title     = {Parallel generation and detection of superoxide and hydrogen peroxide in a fluidic chip},
  series = {Sensors and actuators : B, Chemical},
  volume    = {119},
  journal   = {Sensors and actuators : B, Chemical},
  number    = {1},
  publisher = {Elsevier},
  address   = {Lausanne},
  issn      = {0925-4005},
  doi       = {10.1016/j.snb.2005.11.062},
  pages     = {118 -- 126},
  year      = {2006},
  abstract  = {A fluidic chip system was developed, which combines a stable generation of superoxide radicals and hydrogen peroxide with their sensorial detection. The generation of both reactive oxygen species was achieved by immobilization of xanthine oxidase on controlled pore glass in a reaction chamber. Antioxidants can be introduced into the fluidic chip system by means of mixing chamber. The detection of both species is based on the amperometric principle using a biosensor chip with two working electrodes. As sensing protein for both electrodes cytochrome c was used. The novel system was designed for the quantification of the antioxidant efficiency of different potential scavengers of the respective reactive species in an aqueous medium. Several model antioxidants such as ascorbic acid or catalase have been tested under flow conditions.},
  language  = {en}
}
@article{BeissenhirtzSchellerViezzolietal.2006,
  author    = {Beissenhirtz, Moritz Karl and Scheller, Frieder W. and Viezzoli, Maria Silvia and Lisdat, Fred},
  title     = {Engineered superoxide dismutase monomers for superoxide biosensor applications},
  issn      = {0003-2700},
  doi       = {10.1021/Ac051465g},
  year      = {2006},
  abstract  = {Because of its high reaction rate and specificity, the enzyme superoxide dismutase (SOD) offers great potential for the sensitive quantification of superoxide radicals in electrochemical biosensors. In this work, monomeric mutants of human Cu,Zn-SOD were engineered to contain one or two additional cysteine residues, which could be used to bind the protein to gold surfaces, thus making the use of promotor molecules unnecessary. Six mutants were successfully designed, expressed, and purified. All mutants bound directly to unmodified gold surfaces via the sulfur of the cysteine residues and showed a quasireversible, direct electron transfer to the electrode. Thermodynamic and kinetic parameters of the electron transfer were characterized and showed only slight variations between the individual mutants. For one of the mutants, the interaction with the superoxide radical was studied in more detail. For both partial reactions of the dismutation, an interaction between protein and radical could be shown. In an amperometric biosensorial approach, the SOD-mutant electrode was successfully applied for the detection of superoxide radicals. In the oxidation region, the electrode surpassed the sensitivity of the commonly used cytochrome c electrodes by similar to 1 order of magnitude while not being limited by interferences, but the electrode did not fully reach the sensitivity of dimeric Cu,Zn-SOD immobilized on MPA-modified gold},
  language  = {en}
}
@article{KappBeissenhirtzGeyeretal.2006,
  author    = {Kapp, Andreas and Beissenhirtz, Moritz Karl and Geyer, F. and Scheller, Frieder W. and Viezzoli, Maria Silvia and Lisdat, Fred},
  title     = {Electrochemical and sensorial behaviour of SOD mutants immobilized on gold electrodes in aqueous / organic solvent mixtures},
  issn      = {1040-0397},
  doi       = {10.1002/elan.200603620},
  year      = {2006},
  language  = {en}
}