@article{YanChenKaufmann2016, author = {Yan, Wenhao and Chen, Dijun and Kaufmann, Kerstin}, title = {Efficient multiplex mutagenesis by RNA-guided Cas9 and its use in the characterization of regulatory elements in the AGAMOUS gene}, series = {Plant methods}, volume = {12}, journal = {Plant methods}, publisher = {BioMed Central}, address = {London}, issn = {1746-4811}, doi = {10.1186/s13007-016-0125-7}, pages = {1 -- 9}, year = {2016}, abstract = {Background The efficiency of multiplex editing in plants by the RNA-guided Cas9 system is limited by efficient introduction of its components into the genome and by their activity. The possibility of introducing large fragment deletions by RNA-guided Cas9 tool provides the potential to study the function of any DNA region of interest in its 'endogenous' environment. Results Here, an RNA-guided Cas9 system was optimized to enable efficient multiplex editing in Arabidopsis thaliana. We demonstrate the flexibility of our system for knockout of multiple genes, and to generate heritable large-fragment deletions in the genome. As a proof of concept, the function of part of the second intron of the flower development gene AGAMOUS in Arabidopsis was studied by generating a Cas9-free mutant plant line in which part of this intron was removed from the genome. Further analysis revealed that deletion of this intron fragment results 40 \% decrease of AGAMOUS gene expression without changing the splicing of the gene which indicates that this regulatory region functions as an activator of AGAMOUS gene expression. Conclusions Our modified RNA-guided Cas9 system offers a versatile tool for the functional dissection of coding and non-coding DNA sequences in plants.}, language = {en} } @article{PuigSamperGarrido2016, author = {Puig-Samper, Miguel {\´A}ngel and Garrido, Elisa}, title = {The presentation of the results of Alexander von Humboldt's voyage to Carlos IV}, series = {HiN : Alexander von Humboldt im Netz ; International Review for Humboldtian Studies}, volume = {XVII}, journal = {HiN : Alexander von Humboldt im Netz ; International Review for Humboldtian Studies}, number = {32}, publisher = {Universit{\"a}tsverlag Potsdam}, address = {Potsdam}, issn = {1617-5239}, doi = {10.18443/227}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-90788}, pages = {52 -- 64}, year = {2016}, abstract = {Ever since our first research into Alexander von Humboldt's stay in Spain, the absence of an ensuing relationship between the wise Prussian and the Spanish Crown and Authorities had always surprised us. On starting new research, we found that indeed he sent his first work to Carlos IV from Rome accompanied by a letter of gratitude for the protection he had received during his American trip and submission to the Spanish Crown, which we now present. This first literary fruit of his voyage, which Alexander von Humboldt alluded to in the letter is the first instalment of his work Plantes {\´E}quinoxiales, Recueillies au Mexique, dans l'ile de Cuba, dans les provinces de Caracas, de Cumana etc., published in Paris in 1805.}, language = {en} } @article{BeurskensSteinbergAntoniewiczetal.2016, author = {Beurskens, Rainer and Steinberg, Fabian and Antoniewicz, Franziska and Wolff, Wanja and Granacher, Urs}, title = {Neural Correlates of Dual-Task Walking}, series = {Neural plasticity}, volume = {2016}, journal = {Neural plasticity}, publisher = {Hindawi}, address = {New York}, doi = {10.1155/2016/8032180}, pages = {1 -- 9}, year = {2016}, abstract = {Walking while concurrently performing cognitive and/or motor interference tasks is the norm rather than the exception during everyday life and there is evidence from behavioral studies that it negatively affects human locomotion. However, there is hardly any information available regarding the underlying neural correlates of single- and dual-task walking. We had 12 young adults (23.8 ± 2.8 years) walk while concurrently performing a cognitive interference (CI) or a motor interference (MI) task. Simultaneously, neural activation in frontal, central, and parietal brain areas was registered using a mobile EEG system. Results showed that the MI task but not the CI task affected walking performance in terms of significantly decreased gait velocity and stride length and significantly increased stride time and tempo-spatial variability. Average activity in alpha and beta frequencies was significantly modulated during both CI and MI walking conditions in frontal and central brain regions, indicating an increased cognitive load during dual-task walking. Our results suggest that impaired motor performance during dual-task walking is mirrored in neural activation patterns of the brain. This finding is in line with established cognitive theories arguing that dual-task situations overstrain cognitive capabilities resulting in motor performance decrements.}, language = {en} } @article{Ette2016, author = {Ette, Ottmar}, title = {Naturaleza y cultura}, series = {HiN : Alexander von Humboldt im Netz ; International Review for Humboldtian Studies}, volume = {XVII}, journal = {HiN : Alexander von Humboldt im Netz ; International Review for Humboldtian Studies}, number = {32}, publisher = {Universit{\"a}tsverlag Potsdam}, address = {Potsdam}, issn = {1617-5239}, doi = {10.18443/235}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-90773}, pages = {29 -- 51}, year = {2016}, abstract = {El presente trabajo gira en torno al inexpugnable v{\´i}nculo entre naturaleza y cultura y la 'no naturalidad' de la primera, producto de las milenarias intervenciones del hombre, subsumido bajo el t{\´e}rmino del 'antropoceno'. Los fil{\´o}sofos franceses Bruno Latour y Philippe Descola supieron destacar, aunque por caminos diferentes, la importancia de este nexo para asegurar la supervivencia del hombre; Bruno Latour centra sus reflexiones en la pol{\´i}tica de la naturaleza y Philippe Descola destaca el car{\´a}cter ecol{\´o}gico de la naturaleza y la cultura. Sin embargo, ambos dejan de lado las literaturas del mundo y su capacidad de atesorar los diversos dise{\~n}os del saber convivir entre hombre y naturaleza y las nociones de sustentabilidad. Descuella adem{\´a}s la inspiraci{\´o}n que Descola encuentra en la figura del gran erudito Alexander von Humboldt, quien en el siglo XIX ya daba fe de la relaci{\´o}n inextricable entre naturaleza y cultura en innumerables testimonios, entre otros, el Chimborazo que, como cuadro global es representativo para entender que la naturaleza desde siempre ha sido cultura y la cultura es inimaginble sin la naturaleza.}, language = {es} } @article{Andress2016, author = {Andress, Reinhard}, title = {Eine Bitte an Thomas Jefferson um Tabaksamen und Tabak}, series = {HiN : Alexander von Humboldt im Netz ; International Review for Humboldtian Studies}, volume = {XVII}, journal = {HiN : Alexander von Humboldt im Netz ; International Review for Humboldtian Studies}, number = {32}, publisher = {Universit{\"a}tsverlag Potsdam}, address = {Potsdam}, issn = {1617-5239}, doi = {10.18443/229}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-90761}, pages = {18 -- 28}, year = {2016}, abstract = {Dieser Beitrag behandelt einen bisher unver{\"o}ffentlichen Brief Alexander von Humboldts an Thomas Jefferson. Der Brief bietet uns einen aufschlussreichen Einblick in die pers{\"o}nlichen, politischen und wissenschaftlichen Netzwerke Humboldts und schließt eine L{\"u}cke in der Humboldt-Jefferson-Korrespondenz.}, language = {de} } @article{Schwarz2016, author = {Schwarz, Ingo}, title = {Opfer f{\"u}r die Wissenschaften „in dem Drange wichtiger {\"o}ffentlicher Begebenheiten"}, series = {HiN : Alexander von Humboldt im Netz ; International Review for Humboldtian Studies}, volume = {XVII}, journal = {HiN : Alexander von Humboldt im Netz ; International Review for Humboldtian Studies}, number = {32}, publisher = {Universit{\"a}tsverlag Potsdam}, address = {Potsdam}, issn = {1617-5239}, doi = {10.18443/231}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-90750}, pages = {5 -- 17}, year = {2016}, language = {de} } @article{SafaviHusainVasishth2016, author = {Safavi, Molood S. and Husain, Samar and Vasishth, Shravan}, title = {Dependency Resolution Difficulty Increases with Distance in Persian Separable Complex Predicates}, series = {Frontiers in psychology}, volume = {7}, journal = {Frontiers in psychology}, publisher = {Frontiers Research Foundation}, address = {Lausanne}, issn = {1664-1078}, doi = {10.3389/fpsyg.2016.00403}, pages = {1 -- 15}, year = {2016}, abstract = {Delaying the appearance of a verb in a noun-verb dependency tends to increase processing difficulty at the verb; one explanation for this locality effect is decay and/or interference of the noun in working memory. Surprisal, an expectation-based account, predicts that delaying the appearance of a verb either renders it no more predictable or more predictable, leading respectively to a prediction of no effect of distance or a facilitation. Recently, Husain et al. (2014) suggested that when the exact identity of the upcoming verb is predictable (strong predictability), increasing argument-verb distance leads to facilitation effects, which is consistent with surprisal; but when the exact identity of the upcoming verb is not predictable (weak predictability), locality effects are seen. We investigated Husain et al.'s proposal using Persian complex predicates (CPs), which consist of a non-verbal element—a noun in the current study—and a verb. In CPs, once the noun has been read, the exact identity of the verb is highly predictable (strong predictability); this was confirmed using a sentence completion study. In two self-paced reading (SPR) and two eye-tracking (ET) experiments, we delayed the appearance of the verb by interposing a relative clause (Experiments 1 and 3) or a long PP (Experiments 2 and 4). We also included a simple Noun-Verb predicate configuration with the same distance manipulation; here, the exact identity of the verb was not predictable (weak predictability). Thus, the design crossed Predictability Strength and Distance. We found that, consistent with surprisal, the verb in the strong predictability conditions was read faster than in the weak predictability conditions. Furthermore, greater verb-argument distance led to slower reading times; strong predictability did not neutralize or attenuate the locality effects. As regards the effect of distance on dependency resolution difficulty, these four experiments present evidence in favor of working memory accounts of argument-verb dependency resolution, and against the surprisal-based expectation account of Levy (2008). However, another expectation-based measure, entropy, which was computed using the offline sentence completion data, predicts reading times in Experiment 1 but not in the other experiments. Because participants tend to produce more ungrammatical continuations in the long-distance condition in Experiment 1, we suggest that forgetting due to memory overload leads to greater entropy at the verb.}, language = {en} } @article{LiuSchadKuschpeletal.2016, author = {Liu, Shuyan and Schad, Daniel and Kuschpel, Maxim S. and Rapp, Michael A. and Heinz, Andreas}, title = {Music and Video Gaming during Breaks}, series = {PLoS one}, volume = {11}, journal = {PLoS one}, number = {3}, publisher = {Public Library of Science}, address = {Lawrence, Kan.}, issn = {1932-6203}, doi = {10.1371/JOURNAL.PONE.0150165}, pages = {1 -- 12}, year = {2016}, abstract = {Different systems for habitual versus goal-directed control are thought to underlie human decision-making. Working memory is known to shape these decision-making systems and their interplay, and is known to support goal-directed decision making even under stress. Here, we investigated if and how decision systems are differentially influenced by breaks filled with diverse everyday life activities known to modulate working memory performance. We used a within-subject design where young adults listened to music and played a video game during breaks interleaved with trials of a sequential two-step Markov decision task, designed to assess habitual as well as goal-directed decision making. Based on a neurocomputational model of task performance, we observed that for individuals with a rather limited working memory capacity video gaming as compared to music reduced reliance on the goal-directed decision-making system, while a rather large working memory capacity prevented such a decline. Our findings suggest differential effects of everyday activities on key decision-making processes.}, language = {en} } @article{WackerPiephoHarwoodetal.2016, author = {Wacker, Alexander and Piepho, Maike and Harwood, John L. and Guschina, Irina A. and Arts, Michael T.}, title = {Light-Induced Changes in Fatty Acid Profiles of Specific Lipid Classes in Several Freshwater Phytoplankton Species}, series = {Frontiers in plant science : FPLS}, volume = {7}, journal = {Frontiers in plant science : FPLS}, publisher = {Frontiers Media}, address = {Lausanne}, issn = {1664-462X}, doi = {10.3389/fpls.2016.00264}, pages = {1 -- 13}, year = {2016}, abstract = {We tested the influence of two light intensities [40 and 300 μmol PAR / (m2s)] on the fatty acid composition of three distinct lipid classes in four freshwater phytoplankton species. We chose species of different taxonomic classes in order to detect potentially similar reaction characteristics that might also be present in natural phytoplankton communities. From samples of the bacillariophyte Asterionella formosa, the chrysophyte Chromulina sp., the cryptophyte Cryptomonas ovata and the zygnematophyte Cosmarium botrytis we first separated glycolipids (monogalactosyldiacylglycerol, digalactosyldiacylglycerol, and sulfoquinovosyldiacylglycerol), phospholipids (phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, and phosphatidylserine) as well as non-polar lipids (triacylglycerols), before analyzing the fatty acid composition of each lipid class. High variation in the fatty acid composition existed among different species. Individual fatty acid compositions differed in their reaction to changing light intensities in the four species. Although no generalizations could be made for species across taxonomic classes, individual species showed clear but small responses in their ecologically-relevant omega-3 and omega-6 polyunsaturated fatty acids (PUFA) in terms of proportions and of per tissue carbon quotas. Knowledge on how lipids like fatty acids change with environmental or culture conditions is of great interest in ecological food web studies, aquaculture, and biotechnology, since algal lipids are the most important sources of omega-3 long-chain PUFA for aquatic and terrestrial consumers, including humans.}, language = {en} } @article{NicenboimLogacevGatteietal.2016, author = {Nicenboim, Bruno and Logacev, Pavel and Gattei, Carolina and Vasishth, Shravan}, title = {When High-Capacity Readers Slow Down and Low-Capacity Readers Speed Up}, series = {Frontiers in psychology}, volume = {7}, journal = {Frontiers in psychology}, publisher = {Frontiers Research Foundation}, address = {Lausanne}, issn = {1664-1078}, doi = {10.3389/fpsyg.2016.00280}, pages = {1 -- 24}, year = {2016}, abstract = {We examined the effects of argument-head distance in SVO and SOV languages (Spanish and German), while taking into account readers' working memory capacity and controlling for expectation (Levy, 2008) and other factors. We predicted only locality effects, that is, a slowdown produced by increased dependency distance (Gibson, 2000; Lewis and Vasishth, 2005). Furthermore, we expected stronger locality effects for readers with low working memory capacity. Contrary to our predictions, low-capacity readers showed faster reading with increased distance, while high-capacity readers showed locality effects. We suggest that while the locality effects are compatible with memory-based explanations, the speedup of low-capacity readers can be explained by an increased probability of retrieval failure. We present a computational model based on ACT-R built under the previous assumptions, which is able to give a qualitative account for the present data and can be tested in future research. Our results suggest that in some cases, interpreting longer RTs as indexing increased processing difficulty and shorter RTs as facilitation may be too simplistic: The same increase in processing difficulty may lead to slowdowns in high-capacity readers and speedups in low-capacity ones. Ignoring individual level capacity differences when investigating locality effects may lead to misleading conclusions.}, language = {en} }