@article{MitzscherlingMacLeanLipusetal.2022,
  author    = {Mitzscherling, Julia and MacLean, Joana and Lipus, Daniel and Bartholom{\"a}us, Alexander and Mangelsdorf, Kai and Lipski, Andr{\´e} and Roddatis, Vladimir and Liebner, Susanne and Wagner, Dirk},
  title     = {Nocardioides alcanivorans sp. nov., a novel hexadecane-degrading species isolated from plastic waste},
  series = {International journal of systematic and evolutionary microbiology},
  volume    = {72},
  journal   = {International journal of systematic and evolutionary microbiology},
  number    = {4},
  publisher = {Microbiology Society},
  address   = {London},
  issn      = {1466-5026},
  doi       = {10.1099/ijsem.0.005319},
  pages     = {11},
  year      = {2022},
  abstract  = {Strain NGK65(T), a novel hexadecane degrading, non-motile, Gram-positive, rod-to-coccus shaped, aerobic bacterium, was isolated from plastic polluted soil sampled at a landfill. Strain NGK65(T) hydrolysed casein, gelatin, urea and was catalase-positive. It optimally grew at 28 degrees C. in 0-1\% NaCl and at pH 7.5-8.0. Glycerol, D-glucose, arbutin, aesculin, salicin, potassium 5-ketogluconate. sucrose, acetate, pyruvate and hexadecane were used as sole carbon sources. The predominant membrane fatty acids were iso-C-16:0 followed by iso-C(17:)0 and C-18:1 omega 9c. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and hydroxyphosphatidylinositol. The cell-wall peptidoglycan type was A3 gamma, with LL-diaminopimelic acid and glycine as the diagnostic amino acids. MK 8 (H-4) was the predominant menaquinone. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NGK65(T) belongs to the genus Nocardioides (phylum Actinobacteria). appearing most closely related to Nocardioides daejeonensis MJ31(T) (98.6\%) and Nocardioides dubius KSL-104(T) (98.3\%). The genomic DNA G+C content of strain NGK65(T) was 68.2\%. Strain NGK65(T) and the type strains of species involved in the analysis had average nucleotide identity values of 78.3-71.9\% as well as digital DNA-DNA hybridization values between 22.5 and 19.7\%, which clearly indicated that the isolate represents a novel species within the genus Nocardioides. Based on phenotypic and molecular characterization, strain NGK65(T) can clearly be differentiated from its phylogenetic neighbours to establish a novel species, for which the name Nocardioides alcanivorans sp. nov. is proposed. The type strain is NGK65(T) (=DSM 113112(T)=NCCB 100846(T)).},
  language  = {en}
}
@article{BartholomaeusLipusMitzscherlingetal.2022,
  author    = {Bartholom{\"a}us, Alexander and Lipus, Daniel and Mitzscherling, Julia and MacLean, Joana and Wagner, Dirk},
  title     = {Draft Genome Sequence of Nocardioides alcanivorans NGK65(T), a Hexadecane-Degrading Bacterium},
  series = {Microbiology Resource Announcements},
  volume    = {11},
  journal   = {Microbiology Resource Announcements},
  number    = {8},
  publisher = {American Society for Microbiology},
  address   = {Washington},
  issn      = {2576-098X},
  doi       = {10.1128/mra.01213-21},
  pages     = {2},
  year      = {2022},
  abstract  = {The Gram-positive bacterium Nocardioides alcanivorans NGK65(T) was isolated from plastic-polluted soil and cultivated on medium with polyethylene as the single carbon source. Nanopore sequencing revealed the presence of candidate enzymes for the biodegradation of polyethylene. Here, we report the draft genome of this newly described member of the terrestrial plastisphere.},
  language  = {en}
}
@article{LiuAdlerLipusetal.2020,
  author    = {Liu, Qi and Adler, Karsten and Lipus, Daniel and K{\"a}mpf, Horst and Bussert, Robert and Plessen, Birgit and Schulz, Hans-Martin and Krauze, Patryk and Horn, Fabian and Wagner, Dirk and Mangelsdorf, Kai and Alawi, Mashal},
  title     = {Microbial signatures in deep CO2-saturated miocene sediments of the active Hartousov mofette system (NW Czech Republic)},
  series = {Frontiers in microbiology},
  volume    = {11},
  journal   = {Frontiers in microbiology},
  publisher = {Frontiers Media},
  address   = {Lausanne},
  issn      = {1664-302X},
  doi       = {10.3389/fmicb.2020.543260},
  pages     = {21},
  year      = {2020},
  abstract  = {The Hartousov mofette system is a natural CO2 degassing site in the central Cheb Basin (Eger Rift, Central Europe). In early 2016 a 108 m deep core was obtained from this system to investigate the impact of ascending mantle-derived CO2 on indigenous deep microbial communities and their surrounding life habitat. During drilling, a CO2 blow out occurred at a depth of 78.5 meter below surface (mbs) suggesting a CO2 reservoir associated with a deep low-permeable CO2-saturated saline aquifer at the transition from Early Miocene terrestrial to lacustrine sediments. Past microbial communities were investigated by hopanoids and glycerol dialkyl glycerol tetraethers (GDGTs) reflecting the environmental conditions during the time of deposition rather than showing a signal of the current deep biosphere. The composition and distribution of the deep microbial community potentially stimulated by the upward migration of CO2 starting during Mid Pleistocene time was investigated by intact polar lipids (IPLs), quantitative polymerase chain reaction (qPCR), and deoxyribonucleic acid (DNA) analysis. The deep biosphere is characterized by microorganisms that are linked to the distribution and migration of the ascending CO2-saturated groundwater and the availability of organic matter instead of being linked to single lithological units of the investigated rock profile. Our findings revealed high relative abundances of common soil and water bacteria, in particular the facultative, anaerobic and potential iron-oxidizing Acidovorax and other members of the family Comamonadaceae across the whole recovered core. The results also highlighted the frequent detection of the putative sulfate-oxidizing and CO2-fixating genus Sulfuricurvum at certain depths. A set of new IPLs are suggested to be indicative for microorganisms associated to CO2 accumulation in the mofette system.},
  language  = {en}
}