@article{NawazKhanNoacketal.2020,
  author    = {Nawaz, Shiza and Khan, Muhammad Moman and Noack, Jonas and Awan, Asad Bashir and Schiebel, Juliane and Roggenbuck, Dirk and Schierack, Peter and Sarwar, Yasra and Ali, Aamir},
  title     = {Rapid detection of biofilm formation by zoonotic serovars of Salmonella enterica and avian pathogenic E. coli isolates from poultry},
  series = {Pakistan veterinary journal},
  volume    = {40},
  journal   = {Pakistan veterinary journal},
  number    = {4},
  publisher = {University of Agriculture, Faculty of Veterinary Science},
  address   = {Faisalabad},
  issn      = {0253-8318},
  doi       = {10.29261/pakvetj/2020.066},
  pages     = {527 -- 530},
  year      = {2020},
  abstract  = {Biofilms are complex, sessile microbial communities that are problematic in clinical settings due to their association with survival and pathogenicity of bacteria. The biofilm formation supporting conditions for zoonotic serovars of Salmonella and avian pathogenic E. coli (APEC) from poultry have not been well studied yet. Clinical isolates of zoonotic Salmonella and APEC from poultry were evaluated for biofilm formation in four media at 37 degrees C and 40 degrees C after incubation of 48 and 72 hrs. The biofilms formed in 96 well plates were visualized and quantified with a new module of Aklides system using fluorescence microscope coupled with automated VideoScan Technology. After 72 hrs, brain heart infusion at 40 degrees C and Rappaport-Vassiliadis Soya broth at 37 degrees C were found most suitable for APEC and Salmonella biofilm formations respectively. The new information will be useful for further biofilm associated studies particularly for evaluation of antibiofilm compounds and contribute in infection control. (C) 2020 PVJ. All rights reserved},
  language  = {en}
}
@article{AwanSchiebelBoehmetal.2019,
  author    = {Awan, Asad Bashir and Schiebel, Juliane and Boehm, Alexander and Nitschke, Joerg and Sarwar, Yasra and Schierack, Peter and Ali, Aamir},
  title     = {Association of biofilm formation and cytotoxic potential with multidrug resistance in clinical isolates of pseudomonas aeruginosa},
  series = {EXCLI Journal},
  volume    = {18},
  journal   = {EXCLI Journal},
  publisher = {Leibniz Research Centre for Working Environment and Human Factors},
  address   = {Dortmund},
  issn      = {1611-2156},
  doi       = {10.17179/excli2018-1948},
  pages     = {79 -- 90},
  year      = {2019},
  abstract  = {Multidrug resistant (MDR) Pseudomonas aeruginosa having strong biofilm potential and virulence factors are a serious threat for hospitalized patients having compromised immunity In this study, 34 P. aeruginosa isolates of human origin (17 MDR and 17 non-MDR clinical isolates) were checked for biofilm formation potential in enriched and minimal media. The biofilms were detected using crystal violet method and a modified software package of the automated VideoScan screening method. Cytotoxic potential of the isolates was also investigated on HepG2, LoVo and T24 cell lines using automated VideoScan technology. Pulse field gel electrophoresis revealed 10 PFGE types in MDR and 8 in non-MDR isolates. Although all isolates showed biofilm formation potential, strong biofilm formation was found more in enriched media than in minimal media. Eight MDR isolates showed strong biofilm potential in both enriched and minimal media by both detection methods. Strong direct correlation between crystal violet and VideoScan methods was observed in identifying strong biofilm forming isolates. High cytotoxic effect was observed by 4 isolates in all cell lines used while 6 other isolates showed high cytotoxic effect on T24 cell line only. Strong association of multidrug resistance was found with biofilm formation as strong biofilms were observed significantly higher in MDR isolates (p-value < 0.05) than non-MDR isolates. No significant association of cytotoxic potential with multidrug resistance or biofilm formation was found (p-value > 0.05). The MDR isolates showing significant cytotoxic effects and strong biofilm formation impose a serious threat for hospitalized patients with weak immune system.},
  language  = {en}
}