@article{BresselReich2014, author = {Bressel, Lena and Reich, Oliver}, title = {Theoretical and experimental study of the diffuse transmission of light through highly concentrated absorbing and scattering materials Part I: Monte-Carlo simulations}, series = {Journal of quantitative spectroscopy \& radiative transfer}, volume = {146}, journal = {Journal of quantitative spectroscopy \& radiative transfer}, publisher = {Elsevier}, address = {Oxford}, issn = {0022-4073}, doi = {10.1016/j.jqsrt.2014.01.007}, pages = {190 -- 198}, year = {2014}, abstract = {In many technical materials and commercial products like sunscreen or paint high particle and absorber concentrations are present. An important parameter for slabs of these materials is the diffuse transmission of light, which quantifies the total amount of directly and diffusely transmitted light. Due to the high content of scattering particles not only multiple scattering but also additional dependent scattering occurs. Hence, simple analytical models cannot be applied to calculate the diffuse transmission. In this work a Monte-Carlo program for the calculation of the diffuse transmission of light through dispersions in slab-like geometry containing high concentrations of scattering particles and absorbers is presented and discussed in detail. Mie theory is applied for the calculation of the scattering properties of the samples. Additionally, dependent scattering is considered in two different models, the well-known hard sphere model in the Percus-Yevick approximation (HSPYA) and the Yukawa model in the Mean Spherical Approximation (YMSA). Comparative experiments will show the accurateness of the program as well as its applicability to real samples [1]. (C) 2014 Elsevier Ltd. All rights reserved.}, language = {en} } @article{RoethleinMiettinenIgnatova2015, author = {R{\"o}thlein, Christoph and Miettinen, Markus S. and Ignatova, Zoya}, title = {A flexible approach to assess fluorescence decay functions in complex energy transfer systems}, series = {BMC biophysics}, volume = {8}, journal = {BMC biophysics}, publisher = {BioMed Central}, address = {London}, issn = {2046-1682}, doi = {10.1186/s13628-015-0020-z}, pages = {10}, year = {2015}, abstract = {Background: Time-correlated Forster resonance energy transfer (FRET) probes molecular distances with greater accuracy than intensity-based calculation of FRET efficiency and provides a powerful tool to study biomolecular structure and dynamics. Moreover, time-correlated photon count measurements bear additional information on the variety of donor surroundings allowing more detailed differentiation between distinct structural geometries which are typically inaccessible to general fitting solutions. Results: Here we develop a new approach based on Monte Carlo simulations of time-correlated FRET events to estimate the time-correlated single photon counts (TCSPC) histograms in complex systems. This simulation solution assesses the full statistics of time-correlated photon counts and distance distributions of fluorescently labeled biomolecules. The simulations are consistent with the theoretical predictions of the dye behavior in FRET systems with defined dye distances and measurements of randomly distributed dye solutions. We validate the simulation results using a highly heterogeneous aggregation system and explore the conditions to use this tool in complex systems. Conclusion: This approach is powerful in distinguishing distance distributions in a wide variety of experimental setups, thus providing a versatile tool to accurately distinguish between different structural assemblies in highly complex systems.}, language = {en} }