@article{ColasEwenHannemannetal.2012,
  author    = {Colas, Helene and Ewen, Kerstin M. and Hannemann, Frank and Bistolas, Nikitas and Wollenberger, Ursula and Bernhardt, Rita and de Oliveira, Pedro},
  title     = {Direct and mediated electrochemical response of the cytochrome P450 106A2 from Bacillus megaterium ATCC 13368},
  series = {Bioelectrochemistry : an international journal devoted to electrochemical aspects of biology and biological aspects of electrochemistry ; official journal of the Bioelectrochemical Society},
  volume    = {87},
  journal   = {Bioelectrochemistry : an international journal devoted to electrochemical aspects of biology and biological aspects of electrochemistry ; official journal of the Bioelectrochemical Society},
  number    = {5},
  publisher = {Elsevier},
  address   = {Lausanne},
  issn      = {1567-5394},
  doi       = {10.1016/j.bioelechem.2012.01.006},
  pages     = {71 -- 77},
  year      = {2012},
  abstract  = {CYP106A2 is one of only a few known steroid hydroxylases of bacterial origin, which might be interesting for biotechnological applications. Despite the enzyme having been studied for more than 30 years, its physiological function remains elusive. To date, there have been no reports of the redox potential of CYP106A2, which was supposed to be unusually low for a cytochrome P450. In this work we show that cyclic voltammetry is not only suitable to determine the redox potential of challenging proteins such as CYP106A2, measured at - 128 mV vs. NHE, but also to study molecular interactions of the enzyme with different interaction partners via the respective electrochemical responses. The effect of small ligands, such as carbon monoxide and cyanide, was observed on the cyclic voltammograms of CYP106A2. Furthermore, we found that Tween 80 caused a positive shift of the redox potential of immobilised CYP106A2 indicative for water expulsion from the haem environment. Moreover, electron transfer mediation phenomena with biological redox partners (e.g. ferredoxins) were studied. Finally, the influence of two different kinds of substrates on the electrochemical response of CYP106A2 was assessed, aligning observations from spectral and electrochemical studies.},
  language  = {en}
}
@article{BurovaBernhardtPfeil1995,
  author    = {Burova, Tatjana V. and Bernhardt, Rita and Pfeil, Wolfgang},
  title     = {Conformational stability of holo and apo adrenodoxin : a scanning calorimetric study},
  year      = {1995},
  language  = {en}
}
@article{BurovaBeckertUhlmannetal.1996,
  author    = {Burova, Tatjana V. and Beckert, Vita and Uhlmann, Heike and Ristau, Otto and Bernhardt, Rita and Pfeil, Wolfgang},
  title     = {Conformational stability of adrenodoxin mutant proteins},
  year      = {1996},
  language  = {en}
}
@article{PfeilBurovaBeckertetal.1996,
  author    = {Pfeil, Wolfgang and Burova, Tatjana V. and Beckert, Vita and Uhlmann, Heike and Ristau, Otto and Bernhardt, Rita},
  title     = {Conformational stability of adrenodoxin mutant proteins},
  year      = {1996},
  language  = {en}
}
@article{GoderBeckertPfeiletal.1998,
  author    = {Goder, V. and Beckert, Vita and Pfeil, Wolfgang and Bernhardt, Rita},
  title     = {Impact of the presequence of a mitochondrium targeted precursor, preadrenodoxin, on folding, catalytic activity and stability of the protein in vitro},
  year      = {1998},
  language  = {en}
}
@article{JinBernhardtStoeckleinetal.1998,
  author    = {Jin, Wen and Bernhardt, Rita and St{\"o}cklein, Walter F. M. and Scheller, Frieder W.},
  title     = {Direct electron transfer of adrenodoxin-a [2Fe-2S] protein-- and its mutants on modified gold electrode},
  year      = {1998},
  language  = {en}
}
@article{WollenbergerJinBernhardtetal.1998,
  author    = {Wollenberger, Ursula and Jin, Wen and Bernhardt, Rita and Lehmann, Claudia and St{\"o}cklein, Walter F. M. and Brigelius-Floh{\´e}, Regina and Scheller, Frieder W.},
  title     = {Funktionalisierung von Elektroden f{\"u}r den direkten heterogenen Elektrotransfer},
  year      = {1998},
  language  = {de}
}
@article{WilkePartzschBernhardtetal.2004,
  author    = {Wilke, Max and Partzsch, G. M. and Bernhardt, Rita and Lattard, D.},
  title     = {Determination of the iron oxidation state in basaltic glasses using XANES at the K-edge},
  issn      = {0009-2541},
  year      = {2004},
  abstract  = {Fe K-edge X-ray absorption near edge structure (XANES) and Mossbauer spectra were collected on synthetic glasses of basaltic composition and of glasses on the sodium oxide-silica binary to establish a relation between the pre- edge of the XANES at the K-edge and the Fe oxidation state of depolymerised glasses. Charges of sample material were equilibrated at ambient pressure, superliquidus temperatures and oxygen fugacities that were varied over a range of about 15 orders of magnitude. Most experiments were carried out in gas-flow furnaces, either with pure oxygen, air, or different CO/CO2 mixtures. For the most reduced conditions, the samples charges were enclosed together with a pellet of the IQF oxygen buffer in an evacuated silica glass ampoule. Fe3+/SigmaFe x 100 of the samples determined by Mossbauer spectroscopy range between 0\% and 100\%. Position and intensity of the pre-edge centroid position vary strongly depending on the Fe oxidation state. The pre-edge centroid position and the Fe oxidation state determined by Mossbauer spectroscopy are nonlinearly related and have been fitted by a quadratic polynomial. Alternatively, the ratio of intensities measured at positions sensitive to Fe2+ and Fe3+, respectively, provides an even more sensitive method. Pre- edge intensities of the sample suite indicate average Fe co-ordination between 4 and 6 for all samples regardless of oxidation state. A potential application of the calibration given here opens the possibility of determining Fe oxidation state in glasses of similar compositions with high spatial resolution by use of a Micro-XANES setup (e.g., glass inclusions in natural minerals). (C) 2004 Elsevier B.V. All rights reserved},
  language  = {en}
}
@article{WilkePartzschBernhardtetal.2005,
  author    = {Wilke, Max and Partzsch, G. M. and Bernhardt, Rita and Lattar, D.},
  title     = {Determination of the iron oxidation state in basaltic glasses using XANES at the K-edge},
  issn      = {0009-2541},
  year      = {2005},
  abstract  = {Fe K-edge X-ray absorption near edge structure (XANES) and Mossbauer spectra were collected on synthetic glasses of basaltic composition and of glasses on the sodium oxide-silica binary to establish a relation between the pre- edge of the XANES at the K-edge and the Fe oxidation state of depolymerised glasses. Charges of sample material were equilibrated at ambient pressure, superliquidus temperatures and oxygen fugacities that were varied over a range of about 15 orders of magnitude. Most experiments were carried out in gas-flow furnaces, either with pure oxygen, air, or different CO/CO2 mixtures. For the most reduced conditions, the samples charges were enclosed together with a pellet of the IQF oxygen buffer in an evacuated silica glass ampoule. Fe3+/Sigma Fe x 100 of the samples determined by Mossbauer spectroscopy range between 0\% and 100\%. Position and intensity of the pre-edge centroid position vary strongly depending on the Fe oxidation state. The pre-edge centroid position and the Fe oxidation state determined by Mossbauer spectroscopy are nonlinearly related and have been fitted by a quadratic polynomial. Alternatively, the ratio of intensities measured at positions sensitive to Fe2+ and Fe3+, respectively, provides an even more sensitive method. Pre- edge intensities of the sample suite indicate average Fe co-ordination between 4 and 6 for all samples regardless of oxidation state. A potential application of the calibration given here opens the possibility of determining Fe oxidation state in glasses of similar compositions with high spatial resolution by use of a Micro-XANES setup (e.g., glass inclusions in natural minerals). (c) 2004 Elsevier B.V. All rights reserved},
  language  = {en}
}