@article{SivanesanKalaivaniFischeretal.2012,
  author    = {Sivanesan, Arumugam and Kalaivani, Govindasamy and Fischer, Anna and Stiba, Konstanze and Leimk{\"u}hler, Silke and Weidinger, Inez M.},
  title     = {Complementary surface-enhanced resonance raman Spectroscopic Biodetection of mixed protein solutions by Chitosan- and Silica-Coated Plasmon-Tuned Silver Nanoparticles},
  series = {Analytical chemistry},
  volume    = {84},
  journal   = {Analytical chemistry},
  number    = {13},
  publisher = {American Chemical Society},
  address   = {Washington},
  issn      = {0003-2700},
  doi       = {10.1021/ac301001a},
  pages     = {5759 -- 5764},
  year      = {2012},
  abstract  = {Silver nanoparticles with identical plasmonic properties but different surface functionalities are synthesized and tested as chemically selective surface-enhanced resonance Raman (SERR) amplifiers in a two-component protein solution. The surface plasmon resonances of the particles are tuned to 413 nm to match the molecular resonance of protein heme cofactors. Biocompatible functionalization of the nanoparticles with a thin film of chitosan yields selective SERR enhancement of the anionic protein cytochrome b(5), whereas functionalization with SiO2 amplifies only the spectra of the cationic protein cytochrome c. As a result, subsequent addition of the two differently functionalized particles yields complementary information on the same mixed protein sample solution. Finally, the applicability of chitosan-coated Ag nanoparticles for protein separation was tested by in situ resonance Raman spectroscopy.},
  language  = {en}
}