@article{BrechunArndtWoolley2018,
  author    = {Brechun, Katherine Emily and Arndt, Katja Maren and Woolley, G. Andrew},
  title     = {Selection of protein-protein interactions of desired affinities with a bandpass circuit},
  series = {Journal of molecular biology : JMB},
  volume    = {431},
  journal   = {Journal of molecular biology : JMB},
  number    = {2},
  publisher = {Elsevier},
  address   = {London},
  issn      = {0022-2836},
  doi       = {10.1016/j.jmb.2018.11.011},
  pages     = {391 -- 400},
  year      = {2018},
  abstract  = {We have developed a genetic circuit in Escherichia coli that can be used to select for protein-protein interactions of different strengths by changing antibiotic concentrations in the media. The genetic circuit links protein-protein interaction strength to beta-lactamase activity while simultaneously imposing tuneable positive and negative selection pressure for beta-lactamase activity. Cells only survive if they express interacting proteins with affinities that fall within set high- and low-pass thresholds; i.e. the circuit therefore acts as a bandpass filter for protein-protein interactions. We show that the circuit can be used to recover protein-protein interactions of desired affinity from a mixed population with a range of affinities. The circuit can also be used to select for inhibitors of protein-protein interactions of defined strength. (C) 2018 Elsevier Ltd. All rights reserved.},
  language  = {en}
}