TY  - JOUR
A1  - Malinova, Irina
A1  - Kössler, Stella
A1  - Orawetz, Tom
A1  - Matthes, Ulrike
A1  - Orzechowski, Slawomir
A1  - Koch, Anke
A1  - Fettke, Jörg
T1  - Identification of two Arabidopsis thaliana plasma membrane transporters able to transport glucose 1-phosphate
T2  - Plant & cell physiology
N2  - Primary carbohydrate metabolism in plants includes several sugar and sugar-derivative transport processes. Over recent years, evidences have shown that in starch-related transport processes, in addition to glucose 6-phosphate, maltose, glucose and triose-phosphates, glucose 1-phosphate also plays a role and thereby increases the possible fluxes of sugar metabolites in planta. In this study, we report the characterization of two highly similar transporters, At1g34020 and At4g09810, in Arabidopsis thaliana, which allow the import of glucose 1-phosphate through the plasma membrane. Both transporters were expressed in yeast and were biochemically analyzed to reveal an antiport of glucose 1-phosphate/phosphate. Furthermore, we showed that the apoplast of Arabidopsis leaves contained glucose 1-phosphate and that the corresponding mutant of these transporters had higher glucose 1-phosphate amounts in the apoplast and alterations in starch and starch-related metabolism.
KW  - apoplast
KW  - Arabidopsis thaliana
KW  - glucose 1-phosphate transport
KW  - starch metabolism
KW  - sugar transport
Y1  - 2020
UR  - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/60145
SN  - 0032-0781
SN  - 1471-9053
VL  - 61
IS  - 2
SP  - 381
EP  - 392
PB  - Oxford University Press
CY  - Oxford
ER  -