TY  - JOUR
A1  - Kopp, Johannes Florian
A1  - Müller, Sandra Marie
A1  - Pohl, Gabriele
A1  - Lossow, Kristina
A1  - Kipp, Anna Patricia
A1  - Schwerdtle, Tanja
T1  - A quick and simple method for the determination of six trace elements in mammalian serum samples using ICP-MS/MS
T2  - Journal of trace elements in medicine and biology
N2  - In order to assess the individual trace element status of humans for either medical or scientific purposes, amongst others, blood serum levels are determined. Furthermore, animal models are used to study interactions of trace elements. Most published methods require larger amounts (500-1000 mu L) of serum to achieve a reliable determination of multiple trace elements. However, oftentimes, these amounts of serum cannot be dedicated to a single analysis and the amount available for TE-determination is much lower. Therefore, a published ICP-MS/MS method for trace element determination in serum was miniaturized, optimized and validated for the measurement of Mn, Fe, Cu Zn, I and Se in as little as 50 mu L of human and murine serum and is presented in this work. For validation, recoveries of multiple LOTs and levels from commercially available human reference serum samples were determined, infra- and inter-day variations were assessed and limits of detection and quantification determined. It is shown, that the method is capable of giving accurate and reproducible results for all six elements within the relevant concentration ranges for samples from humans living in central Europe as well as from laboratory mice. As a highlight, the achieved limits of detection and quantification for Mn were found to be at 0.02 mu g/L serum and 0.05 mu g/L serum, respectively, while using an alkaline diluent for the parallel determination of iodine.
Y1  - 2019
UR  - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/48829
SN  - 0946-672X
VL  - 54
SP  - 221
EP  - 225
PB  - Elsevier
CY  - München
ER  -