TY  - JOUR
A1  - Kamm, Birgit
A1  - Kamm, Michael
A1  - Schmidt, Matthias
A1  - Starke, Ines
A1  - Kleinpeter, Erich
T1  - Chemical and biochemical generation of carbohydrates from lignocellulose-feedstock (Lupinus nootkatensis) : quantification of glucose
N2  - Different chemical and enzymatic methods were applied for the hydrolysis of main stems from Lupinus nootkatensis (harvest November 2002). The whole process (all steps) is based on the lignocellulose-feedstock biorefinery regime. The acid hydrolysis of L. was performed with concentrated hydrochloric acid; advantages in this process are exothermic hydrolysis and the possibility of acid recovery. Enzymatic hydrolysis achieved high yields of fermentable carbohydrates (regarding to input cellulose) with high selectivity. However, this way requires the generation of cellulose from L. by chemical pulping. Monosaccharide derivatives thus obtained were identified by their GC retention times and the corresponding MS fragmentation. Hexamethyldisilazane was used as derivatization reagent to prepare the trimethylsilyl derivatives of the carbohydrates and of the degradations products of cellulose from the different fractions. The glucose content was quantified by GC peak integration with respect to an internal standard.
Y1  - 2006
UR  - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/11963
UR  - http://www.sciencedirect.com/science/journal/00456535
SN  - 0045-6535
ER  -