TY  - JOUR
A1  - Sellrie, Frank
A1  - Beck, Michael
A1  - Hildebrandt, Niko
A1  - Micheel, Burkhard
T1  - A homogeneous time-resolved fluoroimmunoassay (TR-FIA) using antibody mediated luminescence quenching
N2  - The determination of low-molecular weight substances (haptens) is demonstrated with a homogeneous time-resolved immunoassay using antibody-induced luminescence quenching. Our novel assay technology uses the newly developed monoclonal antibody (G24-BA9) to quench the luminescence of europium trisbipyridine (EuTBP). We performed a competitive biotin immunoassay including an EuTBP-biotin conjugate, the anti-EuTBP antibody G24-BA9 and streptavidin as assay components. Steric hindrance allows only the binding of either G24-BA9 (to the EuTBP moiety) or streptavidin (to the biotin moiety) to the EuTBP-biotin conjugate. Addition of the analyte biotin resulted in the binding of streptavidin to biotin and a concomitant preferred binding of G24-BA9 to EuTBP-biotin. Since G24-BA9 quenches the luminescence of EuTBP within the conjugate, the luminescence signal could be used to indicate and quantify the presence of free biotin in the system. All experiments were carried out in solution in the presence of 5% serum demonstrating the possibility of using our novel assay for a very fast determination of low molecular weight substances in biological fluids.
Y1  - 2010
UR  - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/31466
UR  - http://www.rsc.org/Publishing/Journals/AY/Index.asp
SN  - 1759-9660
ER  -