TY  - JOUR
A1  - Morris, Penelope J.
A1  - Salt, Carina
A1  - Raila, Jens
A1  - Brenten, Thomas
A1  - Kohn, Barbara
A1  - Schweigert, Florian J.
A1  - Zentek, Juergen
T1  - Safety evaluation of vitamin A in growing dogs
T2  - BRITISH JOURNAL OF NUTRITION
N2  - The safe upper limit for inclusion of vitamin A in complete diets for growing dogs is uncertain, with the result that current recommendations range from 5.24 to 104.80 mu mol retinol (5000 to 100 000 IU vitamin A)/4184 kJ (1000 kcal) metabolisable energy (ME). The aim of the present study was to determine the effect of feeding four concentrations of vitamin A to puppies from weaning until 1 year of age. A total of forty-nine puppies, of two breeds, Labrador Retriever and Miniature Schnauzer, were randomly assigned to one of four treatment groups. Following weaning at 8 weeks of age, puppies were fed a complete food supplemented with retinyl acetate diluted in vegetable oil and fed at 1ml oil/100 g diet to achieve an intake of 5.24, 13.10, 78.60 and 104.80 mu mol retinol (5000, 12 500, 75 000 and 100 000 IU vitamin A)/4184 kJ (1000 kcal) ME. Fasted blood and urine samples were collected at 8, 10, 12, 14, 16, 20, 26, 36 and 52 weeks of age and analysed for markers of vitamin A metabolism and markers of safety including haematological and biochemical variables, bone-specific alkaline phosphatase, cross-linked carboxyterminal telopeptides of type I collagen and dual-energy X-ray absorptiometry. Clinical examinations were conducted every 4 weeks. Data were analysed by means of a mixed model analysis with Bonferroni corrections for multiple endpoints. There was no effect of vitamin A concentration on any of the parameters, with the exception of total serum retinyl esters, and no effect of dose on the number, type and duration of adverse events. We therefore propose that 104.80 mu mol retinol (100 000 IU vitamin A)/4184 kJ (1000 kcal) is a suitable safe upper limit for use in the formulation of diets designed for puppy growth.
KW  - Puppies
KW  - Dogs
KW  - Retinol
KW  - Retinyl esters
KW  - Vitamin A
Y1  - 2012
UR  - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/35509
SN  - 0007-1145
VL  - 108
IS  - 10
SP  - 1800
EP  - 1809
PB  - CAMBRIDGE UNIV PRESS
CY  - CAMBRIDGE
ER  -