TY  - JOUR
A1  - Reim, Tina
A1  - Thamm, Markus
A1  - Rolke, Daniel
A1  - Blenau, Wolfgang
A1  - Scheiner, Ricarda
T1  - Suitability of three common reference genes for quantitative real-time PCR in honey bees
T2  - Apidologie : a quality journal in bee science
N2  - Honey bees are important model organisms for neurobiology, because they display a large array of behaviors. To link behavior with individual gene function, quantitative polymerase chain reaction is frequently used. Comparing gene expression of different individuals requires data normalization using adequate reference genes. These should ideally be expressed stably throughout lifetime. Unfortunately, this is frequently not the case. We studied how well three commonly used reference genes are suited for this purpose and measured gene expression in the brains of honey bees differing in age and social role. Although rpl32 is used most frequently, it only remains stable in expression between newly emerged bees, nurse-aged bees, and pollen foragers but shows a peak at the age of 12 days. The genes gapdh and ef1 alpha-f1, in contrast, are expressed stably in the brain throughout all age groups except newly emerged bees. According to stability software, gapdh was expressed most stably, followed by rpl32 and ef1 alpha-f1.
KW  - gene expression
KW  - quantitative PCR
KW  - reference gene
KW  - stability program
KW  - Apis mellifera
Y1  - 2013
UR  - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/35049
SN  - 0044-8435
VL  - 44
IS  - 3
SP  - 342
EP  - 350
PB  - Springer
CY  - Paris
ER  -