TY  - JOUR
A1  - Zhou, Ying
A1  - Zhang, Ling
A1  - Gui, Jiadong
A1  - Dong, Fang
A1  - Cheng, Sihua
A1  - Mei, Xin
A1  - Zhang, Linyun
A1  - Li, Yongqing
A1  - Su, Xinguo
A1  - Baldermann, Susanne
A1  - Watanabe, Naoharu
A1  - Yang, Ziyin
T1  - Molecular Cloning and Characterization of a Short-Chain Dehydrogenase Showing Activity with Volatile Compounds Isolated from Camellia sinensis
T2  - Plant molecular biology reporter
N2  - Camellia sinensis synthesizes and emits a large variety of volatile phenylpropanoids and benzenoids (VPB). To investigate the enzymes involved in the formation of these VPB compounds, a new C. sinensis short-chain dehydrogenase/reductase (CsSDR) was isolated, cloned, sequenced, and functionally characterized. The complete open reading frame of CsSDR contains 996 nucleotides with a calculated protein molecular mass of 34.5 kDa. The CsSDR recombinant protein produced in Escherichia coli exhibited dehydrogenase-reductase activity towards several major VPB compounds in C. sinensis flowers with a strong preference for NADP/NADPH co-factors, and showed affinity for (R)/(S)-1-phenylethanol (1PE), phenylacetaldehyde, benzaldehyde, and benzyl alcohol, and no affinity for acetophenone (AP) and 2-phenylethanol. CsSDR showed the highest catalytic efficiency towards (R)/(S)-1PE. Furthermore, the transient expression analysis in Nicotiana benthamiana plants validated that CsSDR could convert 1PE to AP in plants. CsSDR transcript level was not significantly affected by floral development and some jasmonic acid-related environmental stress, and CsSDR transcript accumulation was detected in most floral tissues such as receptacle and anther, which were main storage locations of VPB compounds. Our results indicate that CsSDR is expressed in C. sinensis flowers and is likely to contribute to a number of floral VPB compounds including the 1PE derivative AP.
KW  - Camellia sinensis
KW  - 1-Phenylethanol
KW  - Phenylpropanoids
KW  - Short chain dehydrogenase
KW  - Volatile compound
Y1  - 2015
UR  - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/39070
SN  - 0735-9640
SN  - 1572-9818
VL  - 33
IS  - 2
SP  - 253
EP  - 263
PB  - Springer
CY  - New York
ER  -