TY  - JOUR
A1  - Hemme, Dorothea
A1  - Veyel, Daniel
A1  - Muehlhaus, Timo
A1  - Sommer, Frederik
A1  - Jueppner, Jessica
A1  - Unger, Ann-Katrin
A1  - Sandmann, Michael
A1  - Fehrle, Ines
A1  - Schoenfelder, Stephanie
A1  - Steup, Martin
A1  - Geimer, Stefan
A1  - Kopka, Joachim
A1  - Giavalisco, Patrick
A1  - Schroda, Michael
T1  - Systems-wide analysis of acclimation responses to long-term heat stress and recovery in the photosynthetic model organism Chlamydomonas reinhardtii
T2  - The plant cell
N2  - We applied a top-down systems biology approach to understand how Chlamydomonas reinhardtii acclimates to long-term heat stress (HS) and recovers from it. For this, we shifted cells from 25 to 42 degrees C for 24 h and back to 25 degrees C for >= 8 h and monitored abundances of 1856 proteins/protein groups, 99 polar and 185 lipophilic metabolites, and cytological and photosynthesis parameters. Our data indicate that acclimation of Chlamydomonas to long-term HS consists of a temporally ordered, orchestrated implementation of response elements at various system levels. These comprise (1) cell cycle arrest; (2) catabolism of larger molecules to generate compounds with roles in stress protection; (3) accumulation of molecular chaperones to restore protein homeostasis together with compatible solutes; (4) redirection of photosynthetic energy and reducing power from the Calvin cycle to the de novo synthesis of saturated fatty acids to replace polyunsaturated ones in membrane lipids, which are deposited in lipid bodies; and (5) when sinks for photosynthetic energy and reducing power are depleted, resumption of Calvin cycle activity associated with increased photorespiration, accumulation of reactive oxygen species scavengers, and throttling of linear electron flow by antenna uncoupling. During recovery from HS, cells appear to focus on processes allowing rapid resumption of growth rather than restoring pre-HS conditions.
Y1  - 2014
UR  - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/37412
SN  - 1040-4651
SN  - 1532-298X
VL  - 26
IS  - 11
SP  - 4270
EP  - 4297
PB  - American Society of Plant Physiologists
CY  - Rockville
ER  -