TY  - GEN
A1  - Georgiev, Vasil N.
A1  - Grafmüller, Andrea
A1  - Bléger, David
A1  - Hecht, Stefan
A1  - Kunstmann, Ruth Sonja
A1  - Barbirz, Stefanie
A1  - Lipowsky, Reinhard
A1  - Dimova, Rumiana
T1  - Area increase and budding in giant vesicles triggered by light
T2  - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - Biomembranes are constantly remodeled and in cells, these processes are controlled and modulated by an assortment of membrane proteins. Here, it is shown that such remodeling can also be induced by photoresponsive molecules. The morphological control of giant vesicles in the presence of a water-soluble ortho-tetrafluoroazobenzene photoswitch (F-azo) is demonstrated and it is shown that the shape transformations are based on an increase in membrane area and generation of spontaneous curvature. The vesicles exhibit budding and the buds can be retracted by using light of a different wavelength. In the presence of F-azo, the membrane area can increase by more than 5% as assessed from vesicle electrodeformation. To elucidate the underlying molecular mechanism and the partitioning of F-azo in the membrane, molecular dynamics simulations are employed. Comparison with theoretically calculated shapes reveals that the budded shapes are governed by curvature elasticity, that the spontaneous curvature can be decomposed into a local and a nonlocal contribution, and that the local spontaneous curvature is about 1/(2.5 mu m). The results show that exo- and endocytotic events can be controlled by light and that these photoinduced processes provide an attractive method to change membrane area and morphology.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 733 
KW  - azobenzene
KW  - lipid membranes
KW  - molecular dynamics
KW  - photoswitch
KW  - vesicles
Y1  - 2019
UR  - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/42629
UR  - https://nbn-resolving.org/urn:nbn:de:kobv:517-opus4-426298
SN  - 1866-8372
VL  - 5
IS  - 733
ER  -