TY  - JOUR
A1  - Garcia, Sarahi L.
A1  - McMahon, Katherine D.
A1  - Grossart, Hans-Peter
A1  - Warnecke, Falk
T1  - Successful enrichment of the ubiquitous freshwater acI Actinobacteria
T2  - Environmental microbiology reports
N2  - Actinobacteria of the acI lineage are often the numerically dominant bacterial phylum in surface freshwaters, where they can account for >50% of total bacteria. Despite their abundance, there are no described isolates. In an effort to obtain enrichment of these ubiquitous freshwater Actinobacteria, diluted freshwater samples from Lake Grosse Fuchskuhle, Germany, were incubated in 96-well culture plates. With this method, a successful enrichment containing high abundances of a member of the lineage acI was established. Phylogenetic classification showed that the acIActinobacteria of the enrichment belonged to the acI-B2 tribe, which seems to prefer acidic lakes. This enrichment grows to low cell densities and thus the oligotrophic nature of acI-B2 was confirmed.
Y1  - 2014
UR  - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/38119
SN  - 1758-2229
VL  - 6
IS  - 1
SP  - 21
EP  - 27
PB  - Wiley-Blackwell
CY  - Hoboken
ER  -