TY  - JOUR
A1  - Olejko, Lydia
A1  - Cywinski, Piotr J.
A1  - Bald, Ilko
T1  - Ion-Selective formation of a guanine quadruplex on DNA origami structures
T2  - Angewandte Chemie : a journal of the Gesellschaft Deutscher Chemiker ; International edition
N2  - DNA origami nanostructures are a versatile tool that can be used to arrange functionalities with high local control to study molecular processes at a single-molecule level. Here, we demonstrate that DNA origami substrates can be used to suppress the formation of specific guanine (G) quadruplex structures from telomeric DNA. The folding of telomeres into G-quadruplex structures in the presence of monovalent cations (e.g. Na+ and K+) is currently used for the detection of K+ ions, however, with insufficient selectivity towards Na+. By means of FRET between two suitable dyes attached to the 3- and 5-ends of telomeric DNA we demonstrate that the formation of G-quadruplexes on DNA origami templates in the presence of sodium ions is suppressed due to steric hindrance. Hence, telomeric DNA attached to DNA origami structures represents a highly sensitive and selective detection tool for potassium ions even in the presence of high concentrations of sodium ions.
KW  - DNA nanotechnology
KW  - FRET
KW  - G-quadruplexes
KW  - nanostructures
KW  - self-assembly
Y1  - 2015
UR  - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/39255
SN  - 1433-7851
SN  - 1521-3773
VL  - 54
IS  - 2
SP  - 673
EP  - 677
PB  - Wiley-VCH
CY  - Weinheim
ER  -