TY  - JOUR
A1  - Zhang, Shuhao
A1  - Bisterfeld, Carolin
A1  - Bramski, Julia
A1  - Vanparijs, Nane
A1  - De Geest, Bruno G.
A1  - Pietruszka, Jörg
A1  - Böker, Alexander
A1  - Reinicke, Stefan
T1  - Biocatalytically Active Thin Films via Self-Assembly of 2-Deoxy-D-ribose-5-phosphate Aldolase-Poly(N-isopropylacrylamide) Conjugates
T2  - Bioconjugate chemistry
N2  - 2-Deoxy-D-ribose-5-phosphate aldolase (DERA) is a biocatalyst that is capable of converting acetaldehyde and a second aldehyde as acceptor into enantiomerically pure mono- and diyhydroxyaldehydes, which are important structural motifs in a number of pharmaceutically active compounds. However, substrate as well as product inhibition requires a more-sophisticated process design for the synthesis of these motifs. One way to do so is to the couple aldehyde conversion with transport processes, which, in turn, would require an immobilization of the enzyme within a thin film that can be deposited on a membrane support. Consequently, we developed a fabrication process for such films that is based on the formation of DERA-poly(N-isopropylacrylamide) conjugates that are subsequently allowed to self-assemble at an air-water interface to yield the respective film. In this contribution, we discuss the conjugation conditions, investigate the interfacial properties of the conjugates, and, finally, demonstrate a successful film formation under the preservation of enzymatic activity.
Y1  - 2017
UR  - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/54223
SN  - 1043-1802
VL  - 29
IS  - 1
SP  - 104
EP  - 116
PB  - American Chemical Society
CY  - Washington
ER  -