TY  - JOUR
A1  - Geissler, Daniel
A1  - Stufler, Stefan
A1  - Löhmannsröben, Hans-Gerd
A1  - Hildebrandt, Niko
T1  - Six-color time-resolved forster resonance energy transfer for ultrasensitive multiplexed biosensing
T2  - Journal of the American Chemical Society
N2  - Simultaneous monitoring of multiple molecular interactions and multiplexed detection of several diagnostic biomarkers at very low concentrations have become important issues in advanced biological and chemical sensing. Here we present an optically multiplexed six-color Forster resonance energy transfer (FRET) biosensor for simultaneous monitoring of five different individual binding events. We combined simultaneous FRET from one Tb complex to five different organic dyes measured in a filter-based time-resolved detection format with a sophisticated spectral crosstalk correction, which results in very efficient background suppression. The advantages and robustness of the multiplexed FRET sensor were exemplified by analyzing a 15-component lung cancer immunoassay involving 10 different antibodies and five different tumor markers in a single 50 mu L human serum sample. The multiplexed biosensor offers clinically relevant detection limits in the low picomolar (ng/mL) concentration range for all five markers, thus providing an effective early screening tool for lung cancer with the possibility of distinguishing small-cell from non-small-cell lung carcinoma. This novel technology will open new doors for multiple biomarker diagnostics as well as multiplexed real-time imaging and spectroscopy.
Y1  - 2013
UR  - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/35256
SN  - 0002-7863
VL  - 135
IS  - 3
SP  - 1102
EP  - 1109
PB  - American Chemical Society
CY  - Washington
ER  -