TY  - JOUR
A1  - Garz, Andreas
A1  - Sandmann, Michael
A1  - Rading, Michael
A1  - Ramm, Sascha
A1  - Menzel, Ralf
A1  - Steup, Martin
T1  - Cell-to-cell diversity in a synchronized chlamydomonas culture as revealed by single-cell analyses
T2  - Biophysical journal
N2  - In a synchronized photoautotrophic culture of Chlamydomonas reinhardtii, cell size, cell number, and the averaged starch content were determined throughout the light-dark cycle. For single-cell analyses, the relative cellular starch was quantified by measuring the second harmonic generation (SHG). In destained cells, amylopectin essentially represents the only biophotonic structure. As revealed by various validation procedures, SHG signal intensities are a reliable relative measure of the cellular starch content. During photosynthesis-driven starch biosynthesis, synchronized Chlamydomonas cells possess an unexpected cell-to-cell diversity both in size and starch content, but the starch-related heterogeneity largely exceeds that of size. The cellular volume, starch content, and amount of starch/cell volume obey lognormal distributions. Starch degradation was initiated by inhibiting the photosynthetic electron transport in illuminated cells or by darkening. Under both conditions, the averaged rate of starch degradation is almost constant, but it is higher in illuminated than in darkened cells. At the single-cell level, rates of starch degradation largely differ but are unrelated to the initial cellular starch content. A rate equation describing the cellular starch degradation
Y1  - 2012
UR  - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/35654
SN  - 0006-3495
VL  - 103
IS  - 5
SP  - 1078
EP  - 1086
PB  - Cell Press
CY  - Cambridge
ER  -